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Strain identifier

BacDive ID: 134223

Type strain: Yes

Species: Treponema isoptericolens

Strain Designation: SPIT 5

Strain history: S. Dröge SPIT5.

NCBI tax ID(s): 367456 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 7275

BacDive-ID: 134223

DSM-Number: 18056

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, spiral-shaped

description: Treponema isoptericolens SPIT 5 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from Hindgut of termite.

NCBI tax id

  • NCBI tax id: 367456
  • Matching level: species

strain history

doi: 10.13145/bacdive134223.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/spirochaetota
  • domain: Bacteria
  • phylum: Spirochaetota
  • class: Spirochaetia
  • order: Spirochaetales
  • family: Treponemataceae
  • genus: Treponema
  • species: Treponema isoptericolens
  • full scientific name: Treponema isoptericolens Dröge et al. 2008
  • synonyms

    • @ref: 20215
    • synonym: Helmutkoenigia isoptericolens

@ref: 7275

domain: Bacteria

phylum: Spirochaetes

class: Spirochaetia

order: Spirochaetales

family: Treponemataceae

genus: Treponema

species: Treponema isoptericolens

full scientific name: Treponema isoptericolens Dröge et al. 2008

strain designation: SPIT 5

type strain: yes

Morphology

cell morphology

  • @ref: 31949
  • gram stain: negative
  • cell length: 16 µm
  • cell width: 0.45 µm
  • cell shape: spiral-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7275HELMUTKOENIGIA MEDIUM (DSMZ Medium 843)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium843.pdf
7275TREPONEMA RUMINIS MEDIUM (DSMZ Medium 212a)yeshttps://mediadive.dsmz.de/medium/212aName: TREPONEMA RUMINIS MEDIUM (DSMZ Medium 212a) Composition: Na2SO4 2.77778 g/l Trehalose 1.88492 g/l Maltose x H2O 1.78571 g/l Sucrose 1.68651 g/l Cellobiose 1.68651 g/l Na2CO3 1.4881 g/l Trypticase peptone 0.992061 g/l Ethanol 0.94246 g/l D-Glucose 0.793651 g/l D-Xylose 0.744048 g/l Na-pyruvate 0.496032 g/l KH2PO4 0.496031 g/l DL-Dithiothreitol 0.396825 g/l NaCl 0.396825 g/l NH4Cl 0.396825 g/l Starch 0.396825 g/l MgCl2 x 6 H2O 0.327381 g/l L-Cysteine HCl x H2O 0.297619 g/l CaCl2 x 2 H2O 0.0496031 g/l HCl 0.00248016 g/l FeCl2 x 4 H2O 0.0014881 g/l NaOH 0.000496032 g/l Sodium resazurin 0.000496031 g/l Pyridoxine hydrochloride 0.000297619 g/l Nicotinic acid 0.000198413 g/l Thiamine-HCl x 2 H2O 0.000198413 g/l CoCl2 x 6 H2O 0.000188492 g/l Vitamin B12 9.92064e-05 g/l Calcium pantothenate 9.92064e-05 g/l MnCl2 x 4 H2O 9.92064e-05 g/l p-Aminobenzoic acid 7.93651e-05 g/l ZnCl2 6.94444e-05 g/l Na2MoO4 x 2 H2O 3.57143e-05 g/l NiCl2 x 6 H2O 2.38095e-05 g/l D-(+)-biotin 1.98413e-05 g/l H3BO3 5.95238e-06 g/l Na2WO4 x 2 H2O 3.96825e-06 g/l Na2SeO3 x 5 H2O 2.97619e-06 g/l CuCl2 x 2 H2O 1.98413e-06 g/l iso-Valeric acid DL-2-Methylbutyric acid iso-Butyric acid n-Valeric acid Butyric acid Propionic acid Acetic acid Vitamin K1 Distilled water

culture temp

@refgrowthtypetemperaturerange
7275positivegrowth30mesophilic
31949positiveoptimum30mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepH
31949positivegrowth6.5-8
31949positiveoptimum7.3

Physiology and metabolism

oxygen tolerance

  • @ref: 31949
  • oxygen tolerance: anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3194922599arabinose+carbon source
3194917057cellobiose+carbon source
3194928757fructose+carbon source
3194917306maltose+carbon source
3194927082trehalose+carbon source
3194918222xylose+carbon source

enzymes

  • @ref: 31949
  • value: alpha-galactosidase
  • activity: +
  • ec: 3.2.1.22

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinenthost species
31949Hindgut of termite
7275hindgut of the termite Incisitermes tabogaeBelizeBLZMiddle and South America
67770Hindgut contents of the lower drywood termite Incisitermes tabogaeIncisitermes tabogae

isolation source categories

Cat1Cat2Cat3
#Host#Arthropoda#Insecta
#Host Body-Site#Gastrointestinal tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_75814.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_613;96_32136;97_40683;98_53014;99_75814&stattab=map
  • Last taxonomy: Treponema isoptericolens subclade
  • 16S sequence: AM182455
  • Sequence Identity:
  • Total samples: 69
  • soil counts: 2
  • aquatic counts: 1
  • animal counts: 66

Safety information

risk assessment

  • @ref: 7275
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7275
  • description: Treponema isoptericolens partial 16S rRNA gene, type strain SPIT5T
  • accession: AM182455
  • length: 1464
  • database: ena
  • NCBI tax ID: 367456

GC content

@refGC-contentmethod
3194947.7
6777047.7high performance liquid chromatography (HPLC)

External links

@ref: 7275

culture collection no.: DSM 18056, JCM 13955

straininfo link

@refpassport
20218http://www.straininfo.net/strains/822721
20218http://www.straininfo.net/strains/822722

literature

  • topic: Phylogeny
  • Pubmed-ID: 18450692
  • title: Treponema isoptericolens sp. nov., a novel spirochaete from the hindgut of the termite Incisitermes tabogae.
  • authors: Droge S, Rachel R, Radek R, Konig H
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.64699-0
  • year: 2008
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Cellulose/metabolism, DNA, Bacterial/analysis/isolation & purification, Digestive System/*microbiology, Genes, rRNA, Genotype, Isoptera/*microbiology, Lignin/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Treponema/*classification/enzymology/genetics/isolation & purification
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
7275Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18056)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18056
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2820510.1099/ijs.0.64699-018450692
31949Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604128205
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta