Strain identifier
BacDive ID: 134223
Type strain: 
Species: Treponema isoptericolens
Strain Designation: SPIT 5
Strain history: S. Dröge SPIT5.
NCBI tax ID(s): 367456 (species)
version 8.1 (current version)
General
@ref: 7275
BacDive-ID: 134223
DSM-Number: 18056
keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-negative, motile, spiral-shaped
description: Treponema isoptericolens SPIT 5 is an anaerobe, mesophilic, Gram-negative bacterium that was isolated from Hindgut of termite.
NCBI tax id
- NCBI tax id: 367456
- Matching level: species
strain history
| @ref | history |
|---|---|
| 7275 | <- S. Dröge, Johannes Gutenberg-Universität Mainz, Germany; SPIT 5 <- S. Dröge {2005} |
| 67770 | S. Dröge SPIT5. |
doi: 10.13145/bacdive134223.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/spirochaetota
- domain: Bacteria
- phylum: Spirochaetota
- class: Spirochaetia
- order: Spirochaetales
- family: Treponemataceae
- genus: Treponema
- species: Treponema isoptericolens
- full scientific name: Treponema isoptericolens Dröge et al. 2008
synonyms
- @ref: 20215
- synonym: Helmutkoenigia isoptericolens
@ref: 7275
domain: Bacteria
phylum: Spirochaetes
class: Spirochaetia
order: Spirochaetales
family: Treponemataceae
genus: Treponema
species: Treponema isoptericolens
full scientific name: Treponema isoptericolens Dröge et al. 2008
strain designation: SPIT 5
type strain: yes
Morphology
cell morphology
- @ref: 31949
- gram stain: negative
- cell length: 16 µm
- cell width: 0.45 µm
- cell shape: spiral-shaped
- motility: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 7275 | HELMUTKOENIGIA MEDIUM (DSMZ Medium 843) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium843.pdf | |
| 7275 | TREPONEMA RUMINIS MEDIUM (DSMZ Medium 212a) | yes | https://mediadive.dsmz.de/medium/212a | Name: TREPONEMA RUMINIS MEDIUM (DSMZ Medium 212a) Composition: Na2SO4 2.77778 g/l Trehalose 1.88492 g/l Maltose x H2O 1.78571 g/l Sucrose 1.68651 g/l Cellobiose 1.68651 g/l Na2CO3 1.4881 g/l Trypticase peptone 0.992061 g/l Ethanol 0.94246 g/l D-Glucose 0.793651 g/l D-Xylose 0.744048 g/l Na-pyruvate 0.496032 g/l KH2PO4 0.496031 g/l DL-Dithiothreitol 0.396825 g/l NaCl 0.396825 g/l NH4Cl 0.396825 g/l Starch 0.396825 g/l MgCl2 x 6 H2O 0.327381 g/l L-Cysteine HCl x H2O 0.297619 g/l CaCl2 x 2 H2O 0.0496031 g/l HCl 0.00248016 g/l FeCl2 x 4 H2O 0.0014881 g/l NaOH 0.000496032 g/l Sodium resazurin 0.000496031 g/l Pyridoxine hydrochloride 0.000297619 g/l Nicotinic acid 0.000198413 g/l Thiamine-HCl x 2 H2O 0.000198413 g/l CoCl2 x 6 H2O 0.000188492 g/l Vitamin B12 9.92064e-05 g/l Calcium pantothenate 9.92064e-05 g/l MnCl2 x 4 H2O 9.92064e-05 g/l p-Aminobenzoic acid 7.93651e-05 g/l ZnCl2 6.94444e-05 g/l Na2MoO4 x 2 H2O 3.57143e-05 g/l NiCl2 x 6 H2O 2.38095e-05 g/l D-(+)-biotin 1.98413e-05 g/l H3BO3 5.95238e-06 g/l Na2WO4 x 2 H2O 3.96825e-06 g/l Na2SeO3 x 5 H2O 2.97619e-06 g/l CuCl2 x 2 H2O 1.98413e-06 g/l iso-Valeric acid DL-2-Methylbutyric acid iso-Butyric acid n-Valeric acid Butyric acid Propionic acid Acetic acid Vitamin K1 Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 7275 | positive | growth | 30 | mesophilic |
| 31949 | positive | optimum | 30 | mesophilic |
| 67770 | positive | growth | 30 | mesophilic |
culture pH
| @ref | ability | type | pH |
|---|---|---|---|
| 31949 | positive | growth | 6.5-8 |
| 31949 | positive | optimum | 7.3 |
Physiology and metabolism
oxygen tolerance
- @ref: 31949
- oxygen tolerance: anaerobe
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 31949 | 22599 | arabinose | + | carbon source |
| 31949 | 17057 | cellobiose | + | carbon source |
| 31949 | 28757 | fructose | + | carbon source |
| 31949 | 17306 | maltose | + | carbon source |
| 31949 | 27082 | trehalose | + | carbon source |
| 31949 | 18222 | xylose | + | carbon source |
enzymes
- @ref: 31949
- value: alpha-galactosidase
- activity: +
- ec: 3.2.1.22
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent | host species |
|---|---|---|---|---|---|
| 31949 | Hindgut of termite | ||||
| 7275 | hindgut of the termite Incisitermes tabogae | Belize | BLZ | Middle and South America | |
| 67770 | Hindgut contents of the lower drywood termite Incisitermes tabogae | Incisitermes tabogae |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Arthropoda | #Insecta |
| #Host Body-Site | #Gastrointestinal tract |
taxonmaps
- @ref: 69479
- File name: preview.99_75814.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_613;96_32136;97_40683;98_53014;99_75814&stattab=map
- Last taxonomy: Treponema isoptericolens subclade
- 16S sequence: AM182455
- Sequence Identity:
- Total samples: 69
- soil counts: 2
- aquatic counts: 1
- animal counts: 66
Safety information
risk assessment
- @ref: 7275
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 7275
- description: Treponema isoptericolens partial 16S rRNA gene, type strain SPIT5T
- accession: AM182455
- length: 1464
- database: ena
- NCBI tax ID: 367456
GC content
| @ref | GC-content | method |
|---|---|---|
| 31949 | 47.7 | |
| 67770 | 47.7 | high performance liquid chromatography (HPLC) |
External links
@ref: 7275
culture collection no.: DSM 18056, JCM 13955
straininfo link
- @ref: 91817
- straininfo: 404838
literature
- topic: Phylogeny
- Pubmed-ID: 18450692
- title: Treponema isoptericolens sp. nov., a novel spirochaete from the hindgut of the termite Incisitermes tabogae.
- authors: Droge S, Rachel R, Radek R, Konig H
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.64699-0
- year: 2008
- mesh: Animals, Bacterial Typing Techniques, Base Composition, Cellulose/metabolism, DNA, Bacterial/analysis/isolation & purification, Digestive System/*microbiology, Genes, rRNA, Genotype, Isoptera/*microbiology, Lignin/metabolism, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity, Treponema/*classification/enzymology/genetics/isolation & purification
- topic2: Genetics
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 7275 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18056) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18056 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 31949 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28205 | 28776041 | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 91817 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID404838.1 | StrainInfo: A central database for resolving microbial strain identifiers |