Strain identifier

BacDive ID: 134103

Type strain: Yes

Species: Lentibacillus juripiscarius

Strain Designation: IS40-3

Strain history: CIP <- 2005, JCM <- 2003, S. Tanasupawat, Chulalongkorn Univ., Bangkok, Thailand: strain IS40-3

NCBI tax ID(s): 1293599 (strain), 257446 (species)

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General

@ref: 42496

BacDive-ID: 134103

DSM-Number: 16577

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped

description: Lentibacillus juripiscarius IS40-3 is an aerobe, spore-forming, mesophilic bacterium that was isolated from fish sauce.

NCBI tax id

NCBI tax idMatching level
257446species
1293599strain

strain history

@refhistory
42496<- T. Itoh, JCM, Riken, Wako, Saitama, Japan <- S. Tanasupawat, Chulalongkorn University, Thailand; IS40-3
67770S. Tanasupawat IS40-3.
120370CIP <- 2005, JCM <- 2003, S. Tanasupawat, Chulalongkorn Univ., Bangkok, Thailand: strain IS40-3

doi: 10.13145/bacdive134103.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Lentibacillus
  • species: Lentibacillus juripiscarius
  • full scientific name: Lentibacillus juripiscarius Namwong et al. 2005

@ref: 42496

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Caryophanales

family: Bacillaceae

genus: Lentibacillus

species: Lentibacillus juripiscarius

full scientific name: Lentibacillus juripiscarius Namwong et al. 2005

strain designation: IS40-3

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31308positive3.75 µm0.45 µmrod-shapedno
69480yes94.127
69480positive100
120370positiverod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
42496BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 80.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
42172MEDIUM 204 - for Halorubrum, Marinococcus and NatrialbayesDistilled water make up to (1000.000 ml);Sodium chloride (200.000 g);Potassium chloride (2.000 g);ManganeseII chloride tetrahydrate (0.360 mg);Magnesium sulphate heptahydrate (20.000 g);Agar (20.000 g);Yeast extract (5.000 g);Ferrous chloride tetrahydrate
120370CIP Medium 204yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=204

culture temp

@refgrowthtypetemperaturerangeconfidence
42496positivegrowth30mesophilic
31308positivegrowth10-45
31308positiveoptimum37mesophilic
42172positivegrowth37
67770positivegrowth37mesophilic
69480thermophilic90.83
120370positivegrowth22-37
120370nogrowth10psychrophilic
120370nogrowth45thermophilic
120370nogrowth55thermophilic

culture pH

@refabilitytypepHPH range
31308positivegrowth05-09alkaliphile
31308positiveoptimum7
120370nogrowth6

Physiology and metabolism

oxygen tolerance

  • @ref: 31308
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
31308yes
69481yes93
69480yes99.448

halophily

@refsaltgrowthtested relationconcentration
31308NaClpositivegrowth03-30 %
31308NaClpositiveoptimum10 %
120370NaClnogrowth0 %
120370NaClnogrowth2 %
120370NaClnogrowth4 %
120370NaClnogrowth6 %
120370NaClnogrowth8 %
120370NaClnogrowth10 %

observation

@refobservation
31308aggregates in clumps
67770quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3130828757fructose+carbon source
3130817234glucose+carbon source
3130817754glycerol+carbon source
3130833942ribose+carbon source
3130817992sucrose+carbon source
3130853426tween 80+carbon source
3130818222xylose+carbon source
3130817632nitrate+reduction
68371Potassium 5-ketogluconate+builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol+builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose+builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
12037016947citrate-carbon source
1203704853esculin-hydrolysis
120370606565hippurate+hydrolysis
12037017632nitrate+reduction
12037016301nitrite-reduction

metabolite production

  • @ref: 120370
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12037015688acetoin-
12037017234glucose-

enzymes

@refvalueactivityec
31308catalase+1.11.1.6
31308cytochrome oxidase+1.9.3.1
120370oxidase+
120370beta-galactosidase+3.2.1.23
120370alcohol dehydrogenase-1.1.1.1
120370gelatinase-
120370amylase-
120370caseinase-3.4.21.50
120370catalase+1.11.1.6
120370tween esterase-
120370gamma-glutamyltransferase-2.3.2.2
120370lecithinase-
120370lipase-
120370lysine decarboxylase-4.1.1.18
120370ornithine decarboxylase-4.1.1.17
120370protease-
120370urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120370-+++-+---+++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120370+---++-+--++/----------+/---------+----------+/-------+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
42496fish sauceThailandTHAAsia
67770Fish sauce (nam-pla) produced in Thailand
120370Food, Fish, sauceThailandTHAAsia

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production

taxonmaps

  • @ref: 69479
  • File name: preview.99_27990.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_13854;97_16868;98_20951;99_27990&stattab=map
  • Last taxonomy: Lentibacillus juripiscarius subclade
  • 16S sequence: AB127980
  • Sequence Identity:
  • Total samples: 209
  • soil counts: 30
  • aquatic counts: 42
  • animal counts: 137

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
424961Risk group (German classification)
1203701Risk group (French classification)

Sequence information

16S sequences

  • @ref: 31308
  • description: Lentibacillus juripiscarius gene for 16S rRNA, partial sequence
  • accession: AB127980
  • length: 1524
  • database: nuccore
  • NCBI tax ID: 1293599

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lentibacillus juripiscarius JCM 121471293599.7wgspatric1293599
66792Lentibacillus juripiscarius JCM 121472728369697draftimg1293599
67770Lentibacillus juripiscarius JCM 12147GCA_001311805contigncbi1293599

GC content

@refGC-contentmethod
3130843
6777043.4high performance liquid chromatography (HPLC)
6777044.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes93no
motileno77.93yes
gram-positiveyes89.879no
anaerobicno98.765yes
aerobicyes93.31yes
halophileyes96.496yes
spore-formingyes83.841yes
glucose-utilyes87.294yes
flagellatedno85.01yes
thermophileno89.025no
glucose-fermentno85.249no

External links

@ref: 42496

culture collection no.: JCM 12147, PCU 229, TISTR 1535, DSM 16577, CGMCC 1.6153, CIP 108664

straininfo link

  • @ref: 91709
  • straininfo: 135119

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15653893Isolation of Lentibacillus salicampi strains and Lentibacillus juripiscarius sp. nov. from fish sauce in Thailand.Namwong S, Tanasupawat S, Smitinont T, Visessanguan W, Kudo T, Itoh TInt J Syst Evol Microbiol10.1099/ijs.0.63272-02005Animals, Bacillaceae/chemistry/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Fermentation, Fishes/*microbiology, Food Microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride, ThailandBiotechnology
Phylogeny16166670Lentibacillus lacisalsi sp. nov., a moderately halophilic bacterium isolated from a saline lake in China.Lim JM, Jeon CO, Song SM, Lee JC, Ju YJ, Xu LH, Jiang CL, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.63721-02005Bacillaceae/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, China, DNA, Bacterial/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium ChlorideGenetics
Phylogeny16902021Lentibacillus halophilus sp. nov., from fish sauce in Thailand.Tanasupawat S, Pakdeeto A, Namwong S, Thawai C, Kudo T, Itoh TInt J Syst Evol Microbiol10.1099/ijs.0.63997-02006Animals, Bacillaceae/chemistry/*classification/genetics/growth & development/isolation & purification, Culture Media, Cyanoacrylates/analysis, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Fermentation, Fish Products/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Sodium Chloride, Species Specificity, Thailand, Vitamin K 2/analogs & derivativesCultivation
Phylogeny18676465Lentibacillus salis sp. nov., a moderately halophilic bacterium isolated from a salt lake.Lee JC, Li WJ, Xu LH, Jiang CL, Kim CJInt J Syst Evol Microbiol10.1099/ijs.0.65545-02008Bacillaceae/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Fresh Water/*microbiology, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Sodium Chloride, Species SpecificityGenetics
Phylogeny25851835Lentibacillus garicola sp. nov., isolated from myeolchi-aekjeot, a Korean fermented anchovy sauce.Jung WY, Lee SH, Jin HM, Jeon COAntonie Van Leeuwenhoek10.1007/s10482-015-0450-22015Aerobiosis, Bacillaceae/*classification/genetics/*isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, *Food Microbiology, Hydrogen-Ion Concentration, Korea, Molecular Sequence Data, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/metabolism, TemperatureBiotechnology
Phylogeny31424384Lentibacillus lipolyticus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi).Booncharoen A, Visessanguan W, Kuncharoen N, Yiamsombut S, Santiyanont P, Mhuantong W, Charoensri S, Rojsitthisak P, Tanasupawat SInt J Syst Evol Microbiol10.1099/ijsem.0.0036582019Animals, Bacillaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Crustacea/microbiology, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Food Microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seafood/*microbiology, Sequence Analysis, DNA, Thailand, Vitamin K 2/analogs & derivatives/chemistryBiotechnology
Phylogeny32279278Lentibacillus cibarius sp. nov., isolated from kimchi, a Korean fermented food.Oh YJ, Kim JY, Jo HE, Park HK, Lim SK, Kwon MS, Choi HJJ Microbiol10.1007/s12275-020-9507-72020Bacillaceae/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Brassica/*microbiology, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Fermented Foods/*microbiology, *Food Microbiology, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryBiotechnology

Reference

@idauthorstitledoi/urlID_cross_referencepubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
31308Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172762328776041
42172Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6318
42496Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-16577Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16577)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
91709Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID135119.1
120370Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108664Collection of Institut Pasteur (CIP 108664)