Strain identifier

BacDive ID: 13386

Type strain: Yes

Species: Pseudonocardia chloroethenivorans

Strain Designation: SL-1

Strain history: DSM 44698 <-- R. P. Herwig SL-1.

NCBI tax ID(s): 1305626 (strain), 191630 (species)

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General

@ref: 11889

BacDive-ID: 13386

DSM-Number: 44698

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Pseudonocardia chloroethenivorans SL-1 is an aerobe, spore-forming, mesophilic bacterium that was isolated from laboratory enrichment from soil sample.

NCBI tax id

NCBI tax idMatching level
191630species
1305626strain

strain history

@refhistory
11889<- R. P. Herwig; SL-1
67770DSM 44698 <-- R. P. Herwig SL-1.

doi: 10.13145/bacdive13386.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Pseudonocardia
  • species: Pseudonocardia chloroethenivorans
  • full scientific name: Pseudonocardia chloroethenivorans Lee et al. 2004

@ref: 11889

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Pseudonocardia

species: Pseudonocardia chloroethenivorans

full scientific name: Pseudonocardia chloroethenivorans Lee et al. 2004

strain designation: SL-1

type strain: yes

Morphology

cell morphology

  • @ref: 29897
  • gram stain: positive
  • motility: no

pigmentation

  • @ref: 29897
  • production: no

multimedia

  • @ref: 11889
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44698.jpg
  • caption: Medium 535 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11889TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
11889ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
11889positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 29897
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29897
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
29897NaClpositivegrowth0-3 %
29897NaClpositiveoptimum1.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-8(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2989722599arabinose+carbon source
2989717057cellobiose+carbon source
2989728757fructose+carbon source
2989717234glucose+carbon source
2989717716lactose+carbon source
2989717306maltose+carbon source
2989729864mannitol+carbon source
2989726546rhamnose+carbon source
2989717814salicin+carbon source
2989727082trehalose+carbon source
2989718222xylose+carbon source

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11889laboratory enrichment from soil sampleSeattle, WashingtonUSAUSANorth America
67770Laboratory enrichment from a soil sampleSeattle, WAUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Engineered#Laboratory#Lab enrichment
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 11889
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 11889
  • description: Pseudonocardia chlorethenivorans 16S ribosomal RNA gene, partial sequence
  • accession: AF454510
  • length: 1444
  • database: ena
  • NCBI tax ID: 191630

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudonocardia chloroethenivorans JCM 12679GCA_001313845contigncbi1305626
66792Pseudonocardia chloroethenivorans JCM 126791305626.4wgspatric1305626
66792Pseudonocardia chloroethenivorans JCM 126792734481963draftimg1305626

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno86.397no
gram-positiveyes93.218yes
anaerobicno97.639yes
halophileno92.996yes
spore-formingno78.833yes
thermophileno95.54no
glucose-utilyes83.136no
aerobicyes93.686yes
flagellatedno98.209yes
glucose-fermentno90.506no

External links

@ref: 11889

culture collection no.: DSM 44698, ATCC BAA 742, JCM 12679, NCIMB 14322

straininfo link

  • @ref: 82583
  • straininfo: 130850

literature

  • topic: Phylogeny
  • Pubmed-ID: 14742470
  • title: Pseudonocardia chloroethenivorans sp. nov., a chloroethene-degrading actinomycete.
  • authors: Lee SB, Strand SE, Stensel HD, Herwig RP
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02488-0
  • year: 2004
  • mesh: Actinomycetales/*classification/growth & development/isolation & purification/metabolism, Biodegradation, Environmental, Biomass, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, *Environmental Pollutants, Molecular Sequence Data, Phenol/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Soil Microbiology, Vinyl Chloride/*pharmacokinetics, Washington
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
11889Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44698)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44698
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29897Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2626928776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
82583Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID130850.1StrainInfo: A central database for resolving microbial strain identifiers