Strain identifier

BacDive ID: 13386

Type strain: Yes

Species: Pseudonocardia chloroethenivorans

Strain Designation: SL-1

Strain history: DSM 44698 <-- R. P. Herwig SL-1.

NCBI tax ID(s): 1305626 (strain), 191630 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9.2 (current version):
version 9.1:
version 9:
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9.2 (current version)

General

@ref: 11889

BacDive-ID: 13386

DSM-Number: 44698

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Pseudonocardia chloroethenivorans SL-1 is an aerobe, spore-forming, mesophilic bacterium that was isolated from laboratory enrichment from soil sample.

NCBI tax id

NCBI tax idMatching level
1305626strain
191630species

strain history

@refhistory
11889<- R. P. Herwig; SL-1
67770DSM 44698 <-- R. P. Herwig SL-1.

doi: 10.13145/bacdive13386.20241212.9.2

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Pseudonocardia
  • species: Pseudonocardia chloroethenivorans
  • full scientific name: Pseudonocardia chloroethenivorans Lee et al. 2004

@ref: 11889

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Pseudonocardia

species: Pseudonocardia chloroethenivorans

full scientific name: Pseudonocardia chloroethenivorans Lee et al. 2004

strain designation: SL-1

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidence
29897positiveno
69480no93.232

pigmentation

  • @ref: 29897
  • production: no

multimedia

  • @ref: 11889
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44698.jpg
  • caption: Medium 535 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11889TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
11889ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water

culture temp

@refgrowthtypetemperature
11889positivegrowth28
67770positivegrowth28

Physiology and metabolism

oxygen tolerance

  • @ref: 29897
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29897
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
29897NaClpositivegrowth0-3 %
29897NaClpositiveoptimum1.5 %

observation

  • @ref: 67770
  • observation: quinones: MK-8(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2989722599arabinose+carbon source
2989717057cellobiose+carbon source
2989728757fructose+carbon source
2989717234glucose+carbon source
2989717716lactose+carbon source
2989717306maltose+carbon source
2989729864mannitol+carbon source
2989726546rhamnose+carbon source
2989717814salicin+carbon source
2989727082trehalose+carbon source
2989718222xylose+carbon source

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11889laboratory enrichment from soil sampleSeattle, WashingtonUSAUSANorth America
67770Laboratory enrichment from a soil sampleSeattle, WAUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Engineered#Laboratory#Lab enrichment
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 11889
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 11889
  • description: Pseudonocardia chlorethenivorans 16S ribosomal RNA gene, partial sequence
  • accession: AF454510
  • length: 1444
  • database: nuccore
  • NCBI tax ID: 191630

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pseudonocardia chloroethenivorans JCM 12679GCA_001313845contigncbi1305626
66792Pseudonocardia chloroethenivorans JCM 126791305626.4wgspatric1305626
66792Pseudonocardia chloroethenivorans JCM 126792734481963draftimg1305626

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes85.141yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no82.713yes
69480spore-formingspore-formingAbility to form endo- or exosporesno72.583no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes77.463yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno89.728no
69480flagellatedmotile2+Ability to perform flagellated movementno93.232yes

External links

@ref: 11889

culture collection no.: DSM 44698, ATCC BAA-742, JCM 12679, NCIMB 14322

straininfo link

  • @ref: 82583
  • straininfo: 130850

literature

  • topic: Phylogeny
  • Pubmed-ID: 14742470
  • title: Pseudonocardia chloroethenivorans sp. nov., a chloroethene-degrading actinomycete.
  • authors: Lee SB, Strand SE, Stensel HD, Herwig RP
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02488-0
  • year: 2004
  • mesh: Actinomycetales/*classification/growth & development/isolation & purification/metabolism, Biodegradation, Environmental, Biomass, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, *Environmental Pollutants, Molecular Sequence Data, Phenol/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Soil Microbiology, Vinyl Chloride/*pharmacokinetics, Washington
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
11889Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44698)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44698
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29897Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2626928776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
82583Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID130850.1StrainInfo: A central database for resolving microbial strain identifiers