Strain identifier
BacDive ID: 133444
Type strain: 
Species: Thermotalea metallivorans
Strain Designation: B2-1
NCBI tax ID(s): 520762 (species)
version 8.1 (current version)
General
@ref: 29020
BacDive-ID: 133444
DSM-Number: 21119
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, Gram-negative, motile, rod-shaped
description: Thermotalea metallivorans B2-1 is an anaerobe, Gram-negative, motile bacterium that was isolated from freshwater .
NCBI tax id
- NCBI tax id: 520762
- Matching level: species
doi: 10.13145/bacdive133444.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Thermotalea
- species: Thermotalea metallivorans
- full scientific name: Thermotalea metallivorans Ogg and Patel 2009
@ref: 29020
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Eubacteriales
family: Clostridiaceae
genus: Thermotalea
species: Thermotalea metallivorans
strain designation: B2-1
type strain: yes
Morphology
cell morphology
- @ref: 29020
- gram stain: negative
- cell length: 3.25 µm
- cell width: 0.65 µm
- cell shape: rod-shaped
- motility: yes
pigmentation
- @ref: 29020
- production: yes
Culture and growth conditions
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 29020 | positive | growth | 30-55 | |
| 29020 | positive | optimum | 55 | thermophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 29020 | positive | growth | 6.5-9 | alkaliphile |
| 29020 | positive | optimum | 8 |
Physiology and metabolism
oxygen tolerance
- @ref: 29020
- oxygen tolerance: anaerobe
spore formation
- @ref: 29020
- spore formation: no
halophily
- @ref: 29020
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: <4 %
observation
- @ref: 29020
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 29020 | 30089 | acetate | + | carbon source |
| 29020 | 22599 | arabinose | + | carbon source |
| 29020 | 18403 | L-arabitol | + | carbon source |
| 29020 | 22653 | asparagine | + | carbon source |
| 29020 | 17057 | cellobiose | + | carbon source |
| 29020 | 16236 | ethanol | + | carbon source |
| 29020 | 28757 | fructose | + | carbon source |
| 29020 | 17234 | glucose | + | carbon source |
| 29020 | 25017 | leucine | + | carbon source |
| 29020 | 17306 | maltose | + | carbon source |
| 29020 | 29864 | mannitol | + | carbon source |
| 29020 | 37684 | mannose | + | carbon source |
| 29020 | 37657 | methyl D-glucoside | + | carbon source |
| 29020 | 17268 | myo-inositol | + | carbon source |
| 29020 | 506227 | N-acetylglucosamine | + | carbon source |
| 29020 | 17272 | propionate | + | carbon source |
| 29020 | 17148 | putrescine | + | carbon source |
| 29020 | 33942 | ribose | + | carbon source |
| 29020 | 17822 | serine | + | carbon source |
| 29020 | 17992 | sucrose | + | carbon source |
| 29020 | 16296 | D-tryptophan | + | carbon source |
| 29020 | 53423 | tween 40 | + | carbon source |
| 29020 | 53426 | tween 80 | + | carbon source |
| 29020 | 18222 | xylose | + | carbon source |
| 29020 | 17632 | nitrate | + | reduction |
Isolation, sampling and environmental information
isolation
- @ref: 29020
- sample type: freshwater (river, lake, pond)
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Freshwater |
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Aquatic | #Pond (small) |
| #Environmental | #Aquatic | #River (Creek) |
taxonmaps
- @ref: 69479
- File name: preview.99_5969.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15791;96_2849;97_3503;98_4433;99_5969&stattab=map
- Last taxonomy: Thermotalea metallivorans subclade
- 16S sequence: EU443727
- Sequence Identity:
- Total samples: 999
- soil counts: 72
- aquatic counts: 112
- animal counts: 804
- plant counts: 11
Sequence information
16S sequences
- @ref: 29020
- description: Thermotalea metallivorans strain B2-1 16S ribosomal RNA gene, partial sequence
- accession: EU443727
- length: 1627
- database: nuccore
- NCBI tax ID: 520762
Genome sequences
- @ref: 66792
- description: Thermotalea metallivorans B2-1
- accession: GCA_001562415
- assembly level: contig
- database: ncbi
- NCBI tax ID: 520762
GC content
- @ref: 29020
- GC-content: 48
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| flagellated | no | 73.436 | no |
| gram-positive | no | 53.549 | yes |
| anaerobic | yes | 99.25 | yes |
| aerobic | no | 98.39 | yes |
| halophile | no | 89.644 | no |
| spore-forming | yes | 84.879 | yes |
| thermophile | yes | 99.368 | yes |
| glucose-util | yes | 86.491 | yes |
| motile | yes | 87.503 | yes |
| glucose-ferment | yes | 51.437 | no |
External links
@ref: 29020
culture collection no.: KCTC 5625, JCM 15105, DSM 21119
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 19406776 | Thermotalea metallivorans gen. nov., sp. nov., a thermophilic, anaerobic bacterium from the Great Artesian Basin of Australia aquifer. | Ogg CD, Patel BK | Int J Syst Evol Microbiol | 10.1099/ijs.0.004218-0 | 2009 | Australia, Bacteria, Anaerobic/*classification/genetics/isolation & purification/physiology, Bacterial Typing Techniques, Base Composition, Culture Media, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fresh Water/*microbiology, Gram-Positive Asporogenous Rods/*classification/genetics/isolation & purification/physiology, *Hot Temperature, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Species Specificity | Enzymology |
| Phylogeny | 25701849 | Anaerosolibacter carboniphilus gen. nov., sp. nov., a strictly anaerobic iron-reducing bacterium isolated from coal-contaminated soil. | Hong H, Kim SJ, Min UG, Lee YJ, Kim SG, Roh SW, Kim JG, Na JG, Rhee SK | Int J Syst Evol Microbiol | 10.1099/ijs.0.000124 | 2015 | Bacterial Typing Techniques, Base Composition, Coal Mining, DNA, Bacterial/genetics, Environmental Pollution, Fatty Acids/chemistry, Gram-Negative Anaerobic Straight, Curved, and Helical Rods/*classification/genetics/isolation & purification, Iron/metabolism, Molecular Sequence Data, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/chemistry | Genetics |
Reference
| @id | authors | title | doi/url | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 29020 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 25450 | 28776041 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 10.1093/nar/gkaa1025 | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ |