Strain identifier

BacDive ID: 133392

Type strain: Yes

Species: Flavobacterium lacunae

Strain Designation: AHQ-46

NCBI tax ID(s): 1591407 (species)

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General

@ref: 25287

BacDive-ID: 133392

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, motile, rod-shaped, colony-forming

description: Flavobacterium lacunae AHQ-46 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from freshwater sample.

NCBI tax id

  • NCBI tax id: 1591407
  • Matching level: species

doi: 10.13145/bacdive133392.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium lacunae
  • full scientific name: Flavobacterium lacunae Chen et al. 2017

@ref: 25287

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium lacunae

strain designation: AHQ-46

type strain: yes

Morphology

cell morphology

  • @ref: 25287
  • gram stain: negative
  • cell length: 1.2-2.8 µm
  • cell width: 0.2-0.4 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 25287
  • colony size: 0.8-1.2 mm
  • colony color: translucent yellow
  • colony shape: circular
  • incubation period: 2 days
  • medium used: R2A agar

pigmentation

  • @ref: 25287
  • production: no
  • name: Flexirubin-type

Culture and growth conditions

culture medium

@refnamegrowth
25287Reasoner's 2A agar (R2A)yes
25287nutritient agaryes
25287tryptic soy agarno

culture temp

@refgrowthtypetemperature
25287positivegrowth20-30
25287positiveoptimum20-25

culture pH

@refabilitytypepH
25287positivegrowth6.5-7.5
25287positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 25287
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
25287NaClpositivegrowth0.0-0.1 %
25287NaCloptimum0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2528717128adipate-assimilation
2528722599arabinose-assimilation
2528717968butyrate-assimilation
2528716947citrate-assimilation
2528717634D-glucose-builds acid from
2528716991dna-hydrolysis
2528724265gluconate-assimilation
2528717234glucose-assimilation
2528725115malate-assimilation
2528717306maltose-assimilation
2528729864mannitol-assimilation
2528737684mannose-assimilation
25287506227N-acetylglucosamine-assimilation
2528717632nitrate-reduction
2528718401phenylacetate-assimilation
2528753424tween 20-hydrolysis
2528753423tween 40-hydrolysis
2528753425tween 60-hydrolysis
25287167632-oxobutanoate+carbon source
25287309162-oxoglutarate+carbon source
25287286442-oxopentanoate+carbon source
2528730089acetate+carbon source
2528785146carboxymethylcellulose+hydrolysis
25287casein+hydrolysis
2528717029chitin+hydrolysis
25287corn oil+hydrolysis
252874853esculin+hydrolysis
252875291gelatin+hydrolysis
2528729985L-glutamate+carbon source
2528717203L-proline+carbon source
2528717115L-serine+carbon source
2528716857L-threonine+carbon source
2528761995lecithin+hydrolysis
2528751850methyl pyruvate+carbon source
2528773784glycyl-l-glutamate+carbon source
2528728017starch+hydrolysis
2528730031succinate+carbon source
2528717748thymidine+carbon source
2528753426tween 80+hydrolysis
2528716704uridine+carbon source

antibiotic resistance

@refChEBIgroup IDmetaboliteis antibioticis resistantresistance conc.is sensitivesensitivity conc.
252874592426trimethoprimyesyes1.25 µg (disc)
25287933226sulfamethoxazoleyesyes23.75 µg (disc)
2528728971ampicillinyesyes10 µg (disc)
2528717698chloramphenicolyesyes30 µg (disc)
2528717833gentamicinyesyes10 µg (disc)
252876104kanamycinyesyes30 µg (disc)
25287100147nalidixic acidyesyes30 µg (disc)
2528728368novobiocinyesyes30 µg (disc)
2528718208penicillin gyesyes10 µg (disc)
2528728077rifampicinyesyes5 µg (disc)
2528717076streptomycinyesyes10 µg (disc)
2528727902tetracyclineyesyes30 µg (disc)

metabolite production

  • @ref: 25287
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
25287acid phosphatase+3.1.3.2
25287alkaline phosphatase+3.1.3.1
25287alpha-chymotrypsin+3.4.21.1
25287alpha-fucosidase-3.2.1.51
25287alpha-galactosidase-3.2.1.22
25287alpha-glucosidase+3.2.1.20
25287alpha-mannosidase-3.2.1.24
25287arginine dihydrolase-3.5.3.6
25287beta-galactosidase-3.2.1.23
25287beta-glucosidase-3.2.1.21
25287beta-glucuronidase-3.2.1.31
25287catalase+1.11.1.6
25287cystine arylamidase+3.4.11.3
25287cytochrome oxidase+1.9.3.1
25287esterase (C 4)+
25287esterase Lipase (C 8)+
25287leucine arylamidase+3.4.11.1
25287lipase (C 14)+
25287N-acetyl-beta-glucosaminidase+3.2.1.52
25287naphthol-AS-BI-phosphohydrolase+
25287trypsin+3.4.21.4
25287urease-3.5.1.5
25287valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25287C15:0 anteiso2
    25287C15:0 2OH4.6
    25287C15:0 3OH4.3
    25287C15:1ω6c1.3
    25287C16:04
    25287C16:0 3OH1.8
    25287C16:1ω6c / C16:1ω7c10.9
    25287C17:0 3OH1
    25287C18:1ω9c1.4
    25287C13:0 iso1.3
    25287C14:0 iso1.9
    25287C15:0 iso14
    25287C15:0 iso 3OH4.3
    25287C15:1 iso G17.1
    25287C16:0 iso4.5
    25287C16:0 iso 3OH5.1
    25287C16:1 iso G1.7
    25287C17:0 iso 3OH10.1
    25287C17:1 iso ω9c / C16:0 10-methyl6.1
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 2
  • software version: Sherlock 6.0
  • library/peak naming table: RTSBA 6
  • system: MIS MIDI
  • instrument: Hewlett-Packard 5890 Series II
  • method/protocol: Sasser 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 25287
  • sample type: freshwater sample
  • geographic location: Lotus Pond, Sun Yat-sen University, KaoshiungCounty
  • country: Taiwan
  • origin.country: TWN
  • continent: Asia
  • latitude: 22.683
  • longitude: 120.296

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Freshwater

Sequence information

16S sequences

  • @ref: 25287
  • description: Flavobacterium sp. AHQ-46 partial 16S rRNA gene, strain AHQ-46
  • accession: LN717318
  • length: 1405
  • database: nuccore
  • NCBI tax ID: 1591407

GC content

  • @ref: 25287
  • GC-content: 32.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 25287

culture collection no.: BCRC 80889, LMG 28710

literature

  • topic: Phylogeny
  • Pubmed-ID: 29162201
  • title: Flavobacterium lacunae sp. nov., isolated from a freshwater pond.
  • authors: Chen WM, Su CL, Sheu SY
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001693
  • year: 2017
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Fresh Water/microbiology, Phospholipids/chemistry, *Phylogeny, Pigmentation, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spermidine/chemistry, Taiwan, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25287W.-M. S. Chen, Ching-Ling,Sheu, Shih-YiFlavobacterium lacunae sp. nov., isolated from a freshwater pond10.1099/ijsem.0.001693IJSEM 67: 875-882 201729162201