Strain identifier

BacDive ID: 133382

Type strain: Yes

Species: Mycobacterium malmesburyense

Strain history: CIP <- 2014, N. Gcebe, ARC Onderstepoort Vet. Inst., Pretoria, South Africa: strain WCM 7299

NCBI tax ID(s): 1566886 (species)

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General

@ref: 25267

BacDive-ID: 133382

keywords: Bacteria, mesophilic, Gram-positive, coccus-shaped

description: Mycobacterium malmesburyense ATCC BAA 2759 is a mesophilic, Gram-positive, coccus-shaped bacterium that was isolated from bovine nasal swab.

NCBI tax id

  • NCBI tax id: 1566886
  • Matching level: species

strain history

  • @ref: 120760
  • history: CIP <- 2014, N. Gcebe, ARC Onderstepoort Vet. Inst., Pretoria, South Africa: strain WCM 7299

doi: 10.13145/bacdive133382.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium malmesburyense
  • full scientific name: Mycobacterium malmesburyense Gcebe et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium malmesburyense

@ref: 25267

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Mycobacteriales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium malmesburyense

type strain: yes

Morphology

cell morphology

  • @ref: 120760
  • gram stain: positive
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 25267
  • colony color: yellow-orange
  • medium used: Löwenstein Jensen

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
25267Löwenstein Jensenyes
25267Middlebrook 7H11 (Becton Dickinson)yessupplemented with 0.1 % OADC (Merck Chemicals) and glycerol
120760CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
25267positivegrowth25mesophilic
25267positivegrowth37mesophilic
25267positivegrowth45thermophilic
25267positiveoptimum37mesophilic

Physiology and metabolism

halophily

  • @ref: 25267
  • salt: NaCl
  • growth: no
  • tested relation: growth
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2526716947citrate-carbon source
2526716899D-mannitol-assimilation
252674853esculin-hydrolysis
2526717268myo-inositol-assimilation
2526730849L-arabinose-assimilation
2526762345L-rhamnose-assimilation
2526753426tween 80+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
252672637amikacinyesyes30 µg (disc)
25267100241ciprofloxacinyesyes10 µg (disc)
2526750845doxycyclineyesyes30 µg (disc)
252673732clarithromycinyesyes15 µg (disc)
2526728864tobramycinyesyes10 µg (disc)
25267471744imipenemyesyes10 µg (disc)
25267209807cefoxitinyesyes10 µg (disc)
252672676amoxicillinyesyes30 µg (disc)

metabolite tests

  • @ref: 25267
  • Chebi-ID: 16947
  • metabolite: citrate
  • citrate test: -

enzymes

@refvalueactivityec
25267catalase+1.11.1.6
25267nitrate reductase+1.7.99.4
25267pyrazinamidase+3.5.1.B15
25267urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentlatitudelongitudegeographic locationisolation date
25267bovine nasal swabSouth AfricaZAFAfrica-3318
120760Animal, Bovine nasal swabSouth AfricaZAFAfricaWestern Cape Province2011-10-24

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Swab
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Oral cavity and airways#Airways

Safety information

risk assessment

  • @ref: 120760
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

GC content

  • @ref: 25267
  • GC-content: 67.4

External links

@ref: 25267

culture collection no.: ATCC BAA 2759, CIP 110822, WCM 7299

straininfo link

  • @ref: 91422
  • straininfo: 413567

literature

  • topic: Phylogeny
  • Pubmed-ID: 27926825
  • title: Mycobacterium malmesburyense sp. nov., a non-tuberculous species of the genus Mycobacterium revealed by multiple gene sequence characterization.
  • authors: Gcebe N, Rutten V, Pittius NGV, Naicker B, Michel A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001678
  • year: 2017
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Buffaloes/*microbiology, Cattle/*microbiology, DNA, Bacterial/genetics, Genes, Bacterial, Mycobacterium/*classification/genetics/isolation & purification, Mycolic Acids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, South Africa
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmedcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25267N. R. Gcebe, V.,van Pittius, N. G.,Naicker, B.,Michel, A.Mycobacterium malmesburyense sp. nov., a non-tuberculous species of the genus Mycobacterium revealed by multiple gene sequence characterization10.1099/ijsem.0.001678IJSEM 67: 832-838 201727926825
91422Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID413567.1
120760Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110822Collection of Institut Pasteur (CIP 110822)