Strain identifier
BacDive ID: 133379
Type strain:
Species: Brevundimonas balnearis
Strain Designation: FDRGB2b
Strain history: <- E. Toth, Eötvös Loránd Univ. Budapest, Hungary; FDRGB2b <- S. Szuróczki and Z. Kéki
NCBI tax ID(s): 1572858 (species)
General
@ref: 64145
BacDive-ID: 133379
DSM-Number: 29841
keywords: 16S sequence, Bacteria, aerobe, motile, rod-shaped, colony-forming
description: Brevundimonas balnearis FDRGB2b is an aerobe, motile, rod-shaped bacterium that forms circular colonies and was isolated from well water of a thermal bath at Budapest.
NCBI tax id
- NCBI tax id: 1572858
- Matching level: species
strain history
- @ref: 64145
- history: <- E. Toth, Eötvös Loránd Univ. Budapest, Hungary; FDRGB2b <- S. Szuróczki and Z. Kéki
doi: 10.13145/bacdive133379.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Alphaproteobacteria
- order: Caulobacterales
- family: Caulobacteraceae
- genus: Brevundimonas
- species: Brevundimonas balnearis
- full scientific name: Brevundimonas balnearis Tóth et al. 2017
@ref: 64145
domain: Bacteria
phylum: Proteobacteria
class: Alphaproteobacteria
order: Rhizobiales
family: Caulobacteraceae
genus: Brevundimonas
species: Brevundimonas balnearis
full scientific name: Brevundimonas balnearis Tóth et al. 2017
strain designation: FDRGB2b
type strain: yes
Morphology
cell morphology
- @ref: 25252
- cell length: 1.5-3.5 µm
- cell width: 0.3-0.7 µm
- cell shape: rod-shaped
- motility: yes
- flagellum arrangement: polar
colony morphology
- @ref: 25252
- colony size: 1-2 mm
- colony color: mango yellow
- colony shape: circular
- medium used: R2A
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
25252 | Reasoner's 2A agar (R2A) | yes | ||
25252 | Trypticase Soy Agar (TSA) | no | ||
64145 | 1/10 R2A MEDIUM (DSMZ Medium 1578) | yes | https://mediadive.dsmz.de/medium/1578 | Name: 1/10 R2A MEDIUM (DSMZ Medium 1578) Composition: Agar 15.0 g/l Casamino acids 0.05 g/l Starch 0.05 g/l Glucose 0.05 g/l Proteose peptone 0.05 g/l Yeast extract 0.05 g/l K2HPO4 0.03 g/l Na-pyruvate 0.03 g/l MgSO4 x 7 H2O 0.005 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
25252 | positive | growth | 10-45 | |
25252 | positive | optimum | 20-30 | |
64145 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
25252 | positive | growth | 7.0-10.0 | alkaliphile |
25252 | positive | optimum | 8.0-9.0 |
Physiology and metabolism
oxygen tolerance
- @ref: 25252
- oxygen tolerance: aerobe
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
25252 | NaCl | positive | growth | 0-1 %(w/v) |
25252 | NaCl | optimum | 0-1 %(w/v) |
observation
- @ref: 25252
- observation: Stalk formation
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
25252 | 16808 | 2-dehydro-D-gluconate | - | builds acid from |
25252 | 58143 | 5-dehydro-D-gluconate | - | builds acid from |
25252 | 27613 | amygdalin | - | builds acid from |
25252 | 18305 | arbutin | - | builds acid from |
25252 | casein | - | hydrolysis | |
25252 | 15963 | ribitol | - | builds acid from |
25252 | 17108 | D-arabinose | - | builds acid from |
25252 | 18333 | D-arabitol | - | builds acid from |
25252 | 17057 | cellobiose | - | builds acid from |
25252 | 15824 | D-fructose | - | builds acid from |
25252 | 28847 | D-fucose | - | builds acid from |
25252 | 12936 | D-galactose | - | builds acid from |
25252 | 17634 | D-glucose | - | builds acid from |
25252 | 17716 | lactose | - | builds acid from |
25252 | 62318 | D-lyxose | - | builds acid from |
25252 | 17306 | maltose | - | builds acid from |
25252 | 16899 | D-mannitol | - | builds acid from |
25252 | 16024 | D-mannose | - | builds acid from |
25252 | 6731 | melezitose | - | builds acid from |
25252 | 28053 | melibiose | - | builds acid from |
25252 | 16634 | raffinose | - | builds acid from |
25252 | 16988 | D-ribose | - | builds acid from |
25252 | 17992 | sucrose | - | builds acid from |
25252 | 17924 | D-sorbitol | - | builds acid from |
25252 | 16443 | D-tagatose | - | builds acid from |
25252 | 16551 | D-trehalose | - | builds acid from |
25252 | 32528 | turanose | - | builds acid from |
25252 | 65327 | D-xylose | - | builds acid from |
25252 | 17113 | erythritol | - | builds acid from |
25252 | 4853 | esculin | - | builds acid from |
25252 | 16813 | galactitol | - | builds acid from |
25252 | 28066 | gentiobiose | - | builds acid from |
25252 | 24265 | gluconate | - | builds acid from |
25252 | 17754 | glycerol | - | builds acid from |
25252 | 28087 | glycogen | - | builds acid from |
25252 | 17268 | myo-inositol | - | builds acid from |
25252 | 15443 | inulin | - | builds acid from |
25252 | 30849 | L-arabinose | - | builds acid from |
25252 | 18403 | L-arabitol | - | builds acid from |
25252 | 18287 | L-fucose | - | builds acid from |
25252 | 62345 | L-rhamnose | - | builds acid from |
25252 | 17266 | L-sorbose | - | builds acid from |
25252 | 65328 | L-xylose | - | builds acid from |
25252 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
25252 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
25252 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
25252 | 506227 | N-acetylglucosamine | - | builds acid from |
25252 | 17632 | nitrate | - | reduction |
25252 | 17814 | salicin | - | builds acid from |
25252 | 28017 | starch | - | builds acid from |
25252 | 28017 | starch | - | hydrolysis |
25252 | 53426 | tween 80 | - | hydrolysis |
25252 | 17151 | xylitol | - | builds acid from |
25252 | 4853 | esculin | + | hydrolysis |
25252 | 5291 | gelatin | + | hydrolysis |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is resistant | is sensitive | group ID |
---|---|---|---|---|---|---|
25252 | 2676 | amoxicillin | yes | yes | ||
25252 | 18208 | penicillin g | yes | yes | ||
25252 | 8309 | polymyxin b | yes | yes | ||
25252 | 3745 | clindamycin | yes | yes | ||
25252 | 28971 | ampicillin | yes | yes | ||
25252 | 7528 | netilmycin | yes | yes | ||
25252 | 471744 | imipenem | yes | yes | ||
25252 | 28001 | vancomycin | yes | yes | ||
25252 | 209807 | cefoxitin | yes | yes | ||
25252 | 63607 | linezolid | yes | yes | ||
25252 | 7507 | neomycin | yes | yes | ||
25252 | 8232 | piperacillin | yes | yes | 16 | |
25252 | 9421 | tazobactam | yes | yes | 16 | |
25252 | 28864 | tobramycin | yes | yes | ||
25252 | 17833 | gentamicin | yes | yes | ||
25252 | 3393 | carbenicillin | yes | yes | ||
25252 | 3515 | cefuroxime | yes | yes | ||
25252 | 43968 | meropenem | yes | yes | ||
25252 | 48923 | erythromycin | yes | yes | ||
25252 | 100241 | ciprofloxacin | yes | yes | ||
25252 | 3480 | cefamandole | yes | yes |
metabolite production
- @ref: 25252
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
25252 | acid phosphatase | +/- | 3.1.3.2 |
25252 | alkaline phosphatase | + | 3.1.3.1 |
25252 | alpha-Amylase | + | 3.2.1.1 |
25252 | alpha-chymotrypsin | +/- | 3.4.21.1 |
25252 | alpha-fucosidase | - | 3.2.1.51 |
25252 | alpha-galactosidase | - | 3.2.1.22 |
25252 | alpha-glucosidase | + | 3.2.1.20 |
25252 | beta-galactosidase | - | 3.2.1.23 |
25252 | beta-glucosidase | - | 3.2.1.21 |
25252 | beta-glucuronidase | - | 3.2.1.31 |
25252 | beta-mannosidase | - | 3.2.1.25 |
25252 | cystine arylamidase | +/- | 3.4.11.3 |
25252 | esterase (C 4) | + | |
25252 | esterase Lipase (C 8) | + | |
25252 | gelatinase | + | |
25252 | leucine arylamidase | + | 3.4.11.1 |
25252 | lipase (C 14) | - | |
25252 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
25252 | naphthol-AS-BI-phosphohydrolase | + | |
25252 | phosphatase | + | |
25252 | phosphohydrolase | + | |
25252 | valine arylamidase | + |
fatty acid profile
fatty acids
@ref fatty acid percentage 25252 C12:0 3OH 1.7 25252 C14:0 3.8 25252 C15:0 2.2 25252 C16:0 12.7 25252 C16:1ω7c 3.1 25252 C17:0 1.1 25252 C17:1ω6c 2.2 25252 C17:1ω8c 2.5 25252 C18:1ω7c 69 - type of FA analysis: whole cell analysis
- incubation medium: 10% R2A
- agar/liquid: agar
- incubation temperature: 28
- incubation time: 3
- software version: Sherlock 6.1
- library/peak naming table: TSBA 40
- system: MIS MIDI
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
25252 | well water of a thermal bath (Gellért bath) at Budapest | Budapest, Hungary | Hungary | HUN | Europe | 2013-10-28 |
64145 | well water of a thermal bath | Budapest, Gellért thermal bath | Hungary | HUN | Europe |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
taxonmaps
- @ref: 69479
- File name: preview.99_72644.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_73;96_180;97_34035;98_50898;99_72644&stattab=map
- Last taxonomy: Brevundimonas balnearis subclade
- 16S sequence: LN651199
- Sequence Identity:
- Total samples: 4240
- soil counts: 444
- aquatic counts: 2336
- animal counts: 1074
- plant counts: 386
Safety information
risk assessment
- @ref: 64145
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 64145
- description: Brevundimonas sp. FDRGB2b partial 16S rRNA gene, strain FDRGB2b, isolate 2
- accession: LN651199
- length: 1399
- database: ena
- NCBI tax ID: 1572858
GC content
- @ref: 64145
- GC-content: 69.8
- method: high performance liquid chromatography (HPLC)
External links
@ref: 64145
culture collection no.: DSM 29841, NCAIM B.02621
straininfo link
- @ref: 91419
- straininfo: 403124
literature
- topic: Phylogeny
- Pubmed-ID: 27995874
- title: Brevundimonas balnearis sp. nov., isolated from the well water of a thermal bath.
- authors: Toth E, Szuroczki S, Keki Z, Kosztik J, Makk J, Boka K, Sproer C, Marialigeti K, Schumann P
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001746
- year: 2017
- mesh: Bacterial Typing Techniques, Base Composition, Caulobacteraceae/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hungary, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Temperature, Ubiquinone/analogs & derivatives/chemistry, *Water Microbiology, *Water Wells
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed | catalogue |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
25252 | E. S. Tóth, S.,Kéki, Z.,Kosztik, J.,Makk, J.,Bóka, K.,Spröer, C.,Márialigeti, K.,Schumann, P. | Brevundimonas balnearis sp. nov., isolated from the well water of a thermal bath | 10.1099/ijsem.0.001746 | IJSEM 67: 1033-1038 2017 | 27995874 | |
64145 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29841 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29841) | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
91419 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID403124.1 |