Strain identifier

BacDive ID: 133374

Type strain: Yes

Species: Polymorphobacter glacialis

Strain Designation: B555-2

Culture col. no.: CGMCC 1.15519, KCTC 52396

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General

@ref: 25242

BacDive-ID: 133374

keywords: rod-shaped, gram-negative, psychrophilic, aerobe, Bacteria, 16S sequence

description: Polymorphobacter glacialis B555-2 is an aerobe, psychrophilic, gram-negative bacterium that was isolated from ice core in Muztagh Glacier.

doi: 10.13145/bacdive133374.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Sphingosinicellaceae
  • genus: Polymorphobacter
  • species: Polymorphobacter glacialis
  • full scientific name: Polymorphobacter glacialis Xing et al. 2017

@ref: 25242

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Sphingomonadaceae

genus: Polymorphobacter

species: Polymorphobacter glacialis

strain designation: B555-2

type strain: yes

Morphology

cell morphology

  • @ref: 25242
  • gram stain: negative
  • cell length: 1.0-1.3 µm
  • cell width: 0.6-0.7 µm
  • cell shape: rod-shaped

colony morphology

  • @ref: 25242
  • colony color: orange
  • incubation period: 7 days
  • medium used: R2A

Culture and growth conditions

culture medium

  • @ref: 25242
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
25242positivegrowth0-20psychrophilic
25242positiveoptimum15psychrophilic

culture pH

@refabilitytypepH
25242positivegrowth7-9
25242positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 25242
  • oxygen tolerance: aerobe

spore formation

  • @ref: 25242
  • spore formation: no

halophily

  • @ref: 25242
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0.6 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2524217128adipate-carbon source
2524217634D-glucose-carbon source
2524216899D-mannitol-carbon source
2524216024D-mannose-carbon source
2524227689decanoate-carbon source
252425291gelatin-hydrolysis
2524230849L-arabinose-carbon source
2524225115malate-carbon source
2524217306maltose-carbon source
25242506227N-acetylglucosamine-carbon source
2524217632nitrate-reduction
2524218401phenylacetate-carbon source
2524216947citrate+carbon source
252424853esculin+hydrolysis
252424853esculin+carbon source
2524253258sodium citrate+carbon source

metabolite production

@refChebi-IDmetaboliteproduction
2524216136hydrogen sulfideno
2524235581indoleno

enzymes

@refvalueactivityec
25242acid phosphatase+/-3.1.3.2
25242alkaline phosphatase+3.1.3.1
25242alpha-chymotrypsin+/-3.4.21.1
25242alpha-fucosidase-3.2.1.51
25242alpha-galactosidase-3.2.1.22
25242alpha-glucosidase-3.2.1.20
25242alpha-mannosidase-3.2.1.24
25242beta-galactosidase-3.2.1.23
25242beta-glucosidase-3.2.1.21
25242beta-glucuronidase-3.2.1.31
25242catalase+1.11.1.6
25242cystine arylamidase-3.4.11.3
25242cytochrome oxidase-1.9.3.1
25242esterase (C 4)+
25242esterase Lipase (C 8)+
25242leucine arylamidase+3.4.11.1
25242lipase (C 14)-
25242N-acetyl-beta-glucosaminidase-3.2.1.52
25242naphthol-AS-BI-phosphohydrolase+/-
25242trypsin-3.4.21.4
25242valine arylamidase+/-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25242C12:0 2OH3.4
    25242C14:0 2OH10.1
    25242C16:08.2
    25242C16:1ω7c / C16:1ω6c42.8
    25242C17:1ω6c4.2
    25242C18:1ω7c / C18:1ω6c28.8
    25242C16:0 iso 3OH1.2
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 15
  • incubation time: 7
  • software version: Sherlock 6.0
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 25242
  • sample type: ice core in Muztagh Glacier
  • geographic location: Muztagh Glacier, Tibet, China
  • country: China
  • origin.country: CHN
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Glacier
#Environmental#Aquatic#Ice
#Environmental#Terrestrial#Core sample

Sequence information

16S sequences

  • @ref: 25242
  • description: 16S rRNA gene sequences
  • accession: KP013180
  • length: 1388
  • database: ena
  • NCBI tax ID: 1614636

GC content

  • @ref: 25242
  • GC-content: 62.1
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 25242

culture collection no.: CGMCC 1.15519, KCTC 52396

Reference

@idauthorstitledoi/urljournal
20215D.Gleim, M.Kracht, N.Weiss et. al.Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes sincehttp://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html
25242T. L. Xing, Yongqin,Wang, Ninglian,Xu, Baiqing,Shen, Liang,Liu, Keshao,Gu, Zhengquan,Guo, Bixi,Zhou, Yuguang,Liu, HongcanPolymorphobacter glacialis sp. nov., isolated from ice core10.1099/ijsem.0.001672IJSEM 67: 617-620 2017