Strain identifier

BacDive ID: 133372

Type strain: Yes

Species: Clostridium guangxiense

Strain Designation: ZGM211

NCBI tax ID(s): 1662055 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9 (current version):
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 9 (current version)

General

@ref: 25220

BacDive-ID: 133372

keywords: 16S sequence, Bacteria, obligate anaerobe, spore-forming, Gram-positive, motile, rod-shaped, colony-forming

description: Clostridium guangxiense ZGM211 is an obligate anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from lake sediment.

NCBI tax id

  • NCBI tax id: 1662055
  • Matching level: species

doi: 10.13145/bacdive133372.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium guangxiense
  • full scientific name: Clostridium guangxiense Zhao et al. 2017

@ref: 25220

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Eubacteriales

family: Clostridiaceae

genus: Clostridium

species: Clostridium guangxiense

strain designation: ZGM211

type strain: yes

Morphology

cell morphology

  • @ref: 25220
  • gram stain: positive
  • cell length: 5.5-6.0 µm
  • cell width: 0.8-1.0 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: lophotrichous

colony morphology

  • @ref: 25220
  • colony size: 2 mm
  • colony color: milk-white
  • colony shape: circular
  • incubation period: 2 days
  • medium used: PYG agar

Culture and growth conditions

culture medium

  • @ref: 25220
  • name: PYG agar
  • growth: yes

culture temp

@refgrowthtypetemperature
25220positivegrowth20.0-45.0
25220positiveoptimum37

culture pH

@refabilitytypepHPH range
25220positivegrowth4.0-8.0acidophile
25220positiveoptimum7
25220positivegrowth5

Physiology and metabolism

oxygen tolerance

  • @ref: 25220
  • oxygen tolerance: obligate anaerobe

spore formation

  • @ref: 25220
  • type of spore: spore
  • spore formation: yes

halophily

@refsalttested relationconcentrationgrowth
25220NaCloptimum0 %
25220NaClmaximum2 %(w/v)
25220NaClgrowth4 %(w/v)no
25220NaClgrowth8 %(w/v)no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2522017057cellobiose-assimilation
252208391D-gluconate-assimilation
2522016899D-mannitol-assimilation
2522017924D-sorbitol-assimilation
2522032735guanidinium chloride-assimilation
2522017716lactose-assimilation
2522037657methyl D-glucoside-assimilation
2522063154N-acetyl-beta-D-mannosamine-assimilation
25220506227N-acetylglucosamine-assimilation
2522016634raffinose-assimilation
2522017814salicin-assimilation
2522017164stachyose-assimilation
2522027082trehalose-assimilation
2522027082trehalose-builds acid from
2522027897tryptophan-energy source
25220309162-oxoglutarate+assimilation
2522013705acetoacetate+assimilation
2522022599arabinose+builds acid from
2522017057cellobiose+builds acid from
252204853esculin+hydrolysis
252205291gelatin+hydrolysis
2522017234glucose+fermentation
2522017234glucose+builds acid from
2522017754glycerol+builds acid from
2522017716lactose+builds acid from
2522017306maltose+builds acid from
2522029864mannitol+builds acid from
2522037684mannose+builds acid from
252206731melezitose+builds acid from
25220100147nalidixic acid+assimilation
2522016634raffinose+builds acid from
2522026546rhamnose+builds acid from
2522017814salicin+builds acid from
2522030911sorbitol+builds acid from
2522017992sucrose+builds acid from
2522017992sucrose+assimilation
2522018222xylose+builds acid from

metabolite production

  • @ref: 25220
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 25220
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
25220acid phosphatase+3.1.3.2
25220alkaline phosphatase+3.1.3.1
25220alpha-chymotrypsin+3.4.21.1
25220alpha-fucosidase-3.2.1.51
25220alpha-galactosidase+3.2.1.22
25220alpha-glucosidase+3.2.1.20
25220alpha-mannosidase-3.2.1.24
25220beta-galactosidase+3.2.1.23
25220beta-glucosidase+3.2.1.21
25220beta-glucuronidase+3.2.1.31
25220catalase-1.11.1.6
25220cystine arylamidase-3.4.11.3
25220cytochrome oxidase-1.9.3.1
25220esterase (C 4)+
25220esterase Lipase (C 8)+
25220leucine arylamidase+3.4.11.1
25220lipase (C 14)-
25220N-acetyl-beta-glucosaminidase+3.2.1.52
25220naphthol-AS-BI-phosphohydrolase+
25220trypsin+3.4.21.4
25220tryptophan deaminase-4.1.99.1
25220valine arylamidase-

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25220C13:0 3-OH / iso-C15:111.8
    25220C13:1 at 12-133.3
    25220C14:020.9
    25220C16:032.3
    25220C16:1ω5c3.4
    25220C16:1ω7c / C16:1ω6c9.3
    25220C18:1ω7c / C18:1ω6c1.9
    25220Cyclo C-17:01.7
    25220iso-C17:1 / anteiso B5.2
    25220C17:1 iso ω10c1.4
  • type of FA analysis: whole cell analysis
  • incubation medium: PYG medium
  • incubation temperature: 35
  • incubation time: 1
  • system: MIS MIDI
  • method/protocol: Sasser 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

  • @ref: 25220
  • sample type: lake sediment
  • sampling date: 2015
  • geographic location: Longtan Lake in Longtan Park of Liuzhou, in Guangxi Province
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 24
  • longitude: 109
  • enrichment culture: PYG medium
  • enrichment culture composition: 10.0 g glucose, 4.0 g peptone, 1.0 g yeast extract, 2.0 g beef extract, 4.0 g NaCl, 1.5 g KH2PO4 , 0.2 g MgCl2.6H2O, 0.1 g FeSO4.7H2O and 0.5 g L-cysteine, distilled water added to 1 litre final volume
  • enrichment culture duration: 2 days
  • enrichment culture temperature: 35
  • isolation procedure: Hungate roll-tube technique

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_24636.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_10972;97_13292;98_16358;99_24636&stattab=map
  • Last taxonomy: Clostridium
  • 16S sequence: KT000268
  • Sequence Identity:
  • Total samples: 1668
  • soil counts: 479
  • aquatic counts: 289
  • animal counts: 780
  • plant counts: 120

Sequence information

16S sequences

  • @ref: 25220
  • description: Clostridium guangxiense strain ZGM211 16S ribosomal RNA gene, partial sequence
  • accession: KT000268
  • length: 1502
  • database: nuccore
  • NCBI tax ID: 1662055

GC content

  • @ref: 25220
  • GC-content: 40.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 25220

culture collection no.: CICC 24070, BCRC 80950

literature

  • topic: Phylogeny
  • Pubmed-ID: 27911258
  • title: Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., two phylogenetically closely related hydrogen-producing species isolated from lake sediment.
  • authors: Zhao X, Li D, Xu S, Guo Z, Zhang Y, Man L, Jiang B, Hu X
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001702
  • year: 2017
  • mesh: Bacteria, Anaerobic/classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, Clostridium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Geologic Sediments/microbiology, Hydrogen/metabolism, Lakes/*chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Enzymology

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25220X. L. Zhao, D.,Xu, S.,Guo, Z.,Zhang, Y.,Man, L.,Jiang, B.,Hu, X.Clostridium guangxiense sp. nov. and Clostridium neuense sp. nov., two phylogenetically closely related hydrogen-producing species isolated from lake sediment10.1099/ijsem.0.001702IJSEM 67: 710-715 201727911258
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/