Strain identifier

BacDive ID: 133348

Type strain: Yes

Species: Roseitalea porphyridii

Strain Designation: MA7-20

Strain history: C. O. Jeon; Dept. of Life Sci., Chung-Ang Univ., South Korea; MA7-20.

NCBI tax ID(s): 1852022 (species)

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General

@ref: 25207

BacDive-ID: 133348

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-negative, motile, rod-shaped

description: Roseitalea porphyridii MA7-20 is an obligate aerobe, Gram-negative, motile bacterium that was isolated from marine red alga, Porphyridium marinum.

NCBI tax id

  • NCBI tax id: 1852022
  • Matching level: species

strain history

  • @ref: 67770
  • history: C. O. Jeon; Dept. of Life Sci., Chung-Ang Univ., South Korea; MA7-20.

doi: 10.13145/bacdive133348.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Phyllobacteriaceae
  • genus: Roseitalea
  • species: Roseitalea porphyridii
  • full scientific name: Roseitalea porphyridii Hyeon et al. 2017

@ref: 25207

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Hyphomicrobiales

family: Phyllobacteriaceae

genus: Roseitalea

species: Roseitalea porphyridii

strain designation: MA7-20

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
25207negative1.3-1.5 µm0.7-0.8 µmrod-shapedyespolar
69480negative99.979

colony morphology

  • @ref: 25207
  • colony color: red

pigmentation

@refproductionname
25207noFlexirubin-type
25207yesCarotenoid

Culture and growth conditions

culture medium

@refnamegrowthcomposition
25207Marine agar (MA)yescontaining 2 % NaCl
25207Reasoner's 2A agar (R2A)yescontaining 2 % NaCl

culture temp

@refgrowthtypetemperaturerange
25207positivegrowth15.0-45.0
25207positiveoptimum30.0-37.0mesophilic
67770positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
25207positivegrowth6.0-10.5alkaliphile
25207positiveoptimum7.0-8.0

Physiology and metabolism

oxygen tolerance

  • @ref: 25207
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.995

halophily

@refsalttested relationconcentrationgrowth
25207NaCloptimum2.0-3.0 %
25207NaClgrowth0.0-7.0 %(w/v)positive

observation

  • @ref: 67770
  • observation: quinones: Q-10

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2520717128adipate-assimilation
25207casein-hydrolysis
2520717634D-glucose-assimilation
2520716899D-mannitol-assimilation
2520716024D-mannose-assimilation
2520727689decanoate-assimilation
252075291gelatin-hydrolysis
2520717234glucose-fermentation
2520730849L-arabinose-assimilation
2520725115malate-assimilation
2520717306maltose-assimilation
25207506227N-acetylglucosamine-assimilation
2520717632nitrate-reduction
2520718401phenylacetate-assimilation
2520732032potassium gluconate-assimilation
2520753258sodium citrate-assimilation
2520728017starch-hydrolysis
2520727897tryptophan-energy source
2520753424tween 20-hydrolysis
2520753426tween 80-hydrolysis
2520718186tyrosine-hydrolysis
252074853esculin+hydrolysis
2520716199urea+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.is resistantresistance conc.
2520728971ampicillinyesyes10 µg (disc)
252073393carbenicillinyesyes100 µg (disc)
2520717833gentamicinyesyes30 µg (disc)
252076472lincomycinyesyes15 µg (disc)
252077507neomycinyesyes30 µg (disc)
2520728368novobiocinyesyes5 µg (disc)
2520716869oleandomycinyesyes15 µg (disc)
2520727902tetracyclineyesyes30 µg (disc)
252076104kanamycinyesyes30 µg (disc)

metabolite production

  • @ref: 25207
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 25207
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
25207acid phosphatase-3.1.3.2
25207alkaline phosphatase+3.1.3.1
25207alpha-chymotrypsin-3.4.21.1
25207alpha-fucosidase-3.2.1.51
25207alpha-galactosidase-3.2.1.22
25207alpha-glucosidase+3.2.1.20
25207alpha-mannosidase-3.2.1.24
25207arginine dihydrolase-3.5.3.6
25207beta-galactosidase+3.2.1.23
25207beta-glucosidase-3.2.1.21
25207beta-glucuronidase-3.2.1.31
25207catalase+1.11.1.6
25207cystine arylamidase-3.4.11.3
25207cytochrome oxidase+1.9.3.1
25207esterase (C 4)+
25207esterase Lipase (C 8)+
25207leucine arylamidase+3.4.11.1
25207lipase (C 14)-
25207N-acetyl-beta-glucosaminidase-3.2.1.52
25207naphthol-AS-BI-phosphohydrolase-
25207trypsin-3.4.21.4
25207tryptophan deaminase-4.1.99.1
25207valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    2520711-Methyl C18:1ω7c17.8
    25207C16:03.7
    25207C16:1ω7c / C16:1ω6c2.4
    25207C18:015
    25207C18:1ω7c / C18:1ω6c51.8
    25207Cyclo C19:0ω8c8.4
  • type of FA analysis: whole cell analysis
  • incubation medium: marine broth (MB)
  • software version: Sherlock 6.0B
  • library/peak naming table: TSBA 6
  • system: MIS MIDI
  • method/protocol: Sasser 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture durationenrichment culture temperatureisolation procedurehost species
25207marine red alga, Porphyridium marinumYellow (West) SeaRepublic of KoreaKORAsia36126.198marine agar (MA;BD)3 days25cultured for 4 weeks, serially diluted in artificial seawater (ASW; 20 g NaCl, 2.9 g MgSO4 , 4.53 g MgCl2.6H2O, 0.64 g KCl and 1.75 g CaCl2.2H2O per litre; incubated under aerobic conditions
67770Marine alga, Porphyridium marinumRepublic of KoreaKORAsiaPorphyridium marinum

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Host#Algae#Red algae

taxonmaps

  • @ref: 69479
  • File name: preview.99_4586.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_2288;97_2775;98_3445;99_4586&stattab=map
  • Last taxonomy: Roseitalea porphyridii subclade
  • 16S sequence: KX268598
  • Sequence Identity:
  • Total samples: 1372
  • soil counts: 275
  • aquatic counts: 1012
  • animal counts: 39
  • plant counts: 46

Sequence information

16S sequences

  • @ref: 25207
  • description: Hoeflea sp. MA 7-20 16S ribosomal RNA gene, partial sequence
  • accession: KX268598
  • length: 1419
  • database: nuccore
  • NCBI tax ID: 1852022

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Roseitalea porphyridii MA7-20GCA_004331955completencbi1852022
66792Roseitalea porphyridii MA7-202837258257completeimg1852022

GC content

@refGC-contentmethod
2520761.5fluorimetric
6777061.5thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileyes87.139no
flagellatedyes52.747yes
gram-positiveno97.807no
anaerobicno96.932no
aerobicyes85.923yes
halophileno53.263no
spore-formingno97.032no
glucose-utilyes75.508yes
thermophileno91.815yes
glucose-fermentno91.7yes

External links

@ref: 25207

culture collection no.: KACC 18807, JCM 31538

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902230
  • title: Roseitalea porphyridii gen. nov., sp. nov., isolated from a red alga, and reclassification of Hoeflea suaedae Chung et al. 2013 as Pseudohoeflea suaedae gen. nov., comb. nov.
  • authors: Hyeon JW, Jeong SE, Baek K, Jeon CO
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001633
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Phospholipids/chemistry, Phyllobacteriaceae/*classification/genetics/isolation & purification, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodophyta/*microbiology, Sequence Analysis, DNA, Ubiquinone/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25207J. W. J. Hyeon, S. E.,Baek, K.,Jeon, C. O.Roseitalea porphyridii gen. nov., sp. nov., isolated from a red alga, and reclassification of Hoeflea suaedae Chung et al. 2013 as Pseudohoeflea suaedae gen. nov., comb. nov10.1099/ijsem.0.001633IJSEM 67: 362-368 201727902230
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1