Strain identifier

BacDive ID: 133345

Type strain: Yes

Species: Alteromonas lipolytica

Strain Designation: JW12

NCBI tax ID(s): 1856405 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 25171

BacDive-ID: 133345

keywords: genome sequence, 16S sequence, Bacteria, aerobe, chemoorganotroph, Gram-negative, motile, rod-shaped, colony-forming

description: Alteromonas lipolytica JW12 is an aerobe, chemoorganotroph, Gram-negative bacterium that forms circular colonies and was isolated from surface seawater.

NCBI tax id

  • NCBI tax id: 1856405
  • Matching level: species

doi: 10.13145/bacdive133345.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Alteromonadales
  • family: Alteromonadaceae
  • genus: Alteromonas
  • species: Alteromonas lipolytica
  • full scientific name: Alteromonas lipolytica Shi et al. 2017

@ref: 25171

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Alteromonadales

family: Alteromonadaceae

genus: Alteromonas

species: Alteromonas lipolytica

strain designation: JW12

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
25171negative1.5-2.2 µm0.7-1.2 µmrod-shapedyes
69480yes97.737
69480negative99.999

colony morphology

  • @ref: 25171
  • colony size: 1.0-2.0 mm
  • colony color: cream
  • colony shape: circular
  • incubation period: 1 day
  • medium used: marine agar (MA)

Culture and growth conditions

culture medium

  • @ref: 25171
  • name: marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
25171positivegrowth15.0-43.0
25171positiveoptimum30mesophilic

culture pH

@refabilitytypepHPH range
25171positivegrowth5.5-9.5alkaliphile
25171positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 25171
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 25171
  • type: chemoorganotroph

spore formation

@refspore formationconfidence
69481no100
69480no99.952

halophily

@refsaltgrowthtested relationconcentration
25171NaClpositivegrowth0.5-10.0 %(w/v)
25171NaCloptimum3 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2517116899D-mannitol-builds acid from
2517116024D-mannose-builds acid from
2517117924D-sorbitol-builds acid from
2517165327D-xylose-builds acid from
2517116236ethanol-builds acid from
251715291gelatin-hydrolysis
2517117268myo-inositol-builds acid from
2517162345L-rhamnose-builds acid from
2517117266L-sorbose-builds acid from
2517115963ribitol-builds acid from
2517128017starch-hydrolysis
2517136219alpha-lactose+builds acid from
2517117057cellobiose+builds acid from
2517112936D-galactose+builds acid from
2517117634D-glucose+builds acid from
251714853esculin+hydrolysis
2517117306maltose+builds acid from
251716731melezitose+builds acid from
2517116634raffinose+builds acid from
2517133942ribose+builds acid from
2517117992sucrose+builds acid from
2517127082trehalose+builds acid from
2517153424tween 20+hydrolysis
2517153423tween 40+hydrolysis
2517153426tween 80+hydrolysis

metabolite production

  • @ref: 25171
  • Chebi-ID: 53388
  • metabolite: polyhydroxybutyrate
  • production: yes

enzymes

@refvalueactivityec
25171acid phosphatase+3.1.3.2
25171alkaline phosphatase+3.1.3.1
25171alpha-chymotrypsin+3.4.21.1
25171alpha-galactosidase+3.2.1.22
25171alpha-glucosidase+3.2.1.20
25171alpha-mannosidase-3.2.1.24
25171beta-glucosidase+3.2.1.21
25171beta-glucuronidase+3.2.1.31
25171catalase-1.11.1.6
25171cystine arylamidase+3.4.11.3
25171cytochrome oxidase+1.9.3.1
25171esterase (C 4)+
25171esterase Lipase (C 8)+
25171leucine arylamidase+3.4.11.1
25171lipase (C 14)-
25171naphthol-AS-BI-phosphohydrolase+
25171trypsin+3.4.21.4
25171urease-3.5.1.5
25171valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    2517110-Methyl C17:00.5
    25171C10:0 3OH0.4
    25171C12:03.6
    25171C12:0 3OH2.6
    25171C12:1 3OH2.8
    25171C13:1 at 12-130.4
    25171C14:03
    25171C14:0 3OH / C16:1 iso I3.3
    25171C15:00.6
    25171C15:1ω8c0.4
    25171C16:026.7
    25171C16:0 3OH0.5
    25171C16:0 n alcohol1
    25171C16:1ω7c / C15:0 iso 2OH34
    25171C16:1ω7c alcohol1.5
    25171C17:00.5
    25171C17:1ω8c1
    25171C18:01.2
    25171C18:1ω7c14.6
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine Agar (MA)
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 0
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 25171
  • sample type: surface seawater
  • geographic location: Arabian Sea
  • enrichment culture: natural seawater agar
  • enrichment culture composition: pH 7.2–7.4; supplemented with 0.05 % peptone (w/v; BD Difco) and 0.01 % yeast extract (w/v; BD Difco)
  • enrichment culture duration: 10 days
  • enrichment culture temperature: 30
  • isolation procedure: standard 10-fold dilution plating technique

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Surface water

taxonmaps

  • @ref: 69479
  • File name: preview.99_7685.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_98;96_261;97_4387;98_5617;99_7685&stattab=map
  • Last taxonomy: Alteromonas
  • 16S sequence: KX146484
  • Sequence Identity:
  • Total samples: 670
  • soil counts: 10
  • aquatic counts: 590
  • animal counts: 66
  • plant counts: 4

Sequence information

16S sequences

  • @ref: 25171
  • description: Alteromonas sp. JW12 16S ribosomal RNA gene, partial sequence
  • accession: KX146484
  • length: 1455
  • database: nuccore
  • NCBI tax ID: 1856405

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Alteromonas lipolytica JW12GCA_001758465scaffoldncbi1856405
66792Alteromonas lipolytica CGMCC 1.15735GCA_014643555contigncbi1856405
66792Alteromonas lipolytica strain CGMCC 1.157351856405.15wgspatric1856405
66792Alteromonas lipolytica JW122834723206draftimg1856405

GC content

  • @ref: 25171
  • GC-content: 48.4
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedyes89.918no
gram-positiveno99.199yes
anaerobicno97.901yes
aerobicyes74.809no
halophileyes51.916no
spore-formingno96.695no
glucose-utilyes88.744no
thermophileno99.692no
motileyes92.649yes
glucose-fermentno82.31no

External links

@ref: 25171

culture collection no.: CGMCC 1.15735, KCTC 52408, MCCC 1K03175

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902291
  • title: Alteromonas lipolytica sp. nov., a poly-beta-hydroxybutyrate-producing bacterium isolated from surface seawater.
  • authors: Shi XL, Wu YH, Jin XB, Wang CS, Xu XW
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001604
  • year: 2017
  • mesh: Alteromonas/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hydroxybutyrates/*metabolism, Indian Ocean, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polyesters/*metabolism, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA
  • topic2: Metabolism

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25171X. L. W. Shi, Y. H.,Jin, X. B.,Wang, C. S.,Xu, X. W.Alteromonas lipolytica sp. nov., a poly-beta-hydroxybutyrate-producing bacterium isolated from surface seawater10.1099/ijsem.0.001604IJSEM 67: 237-242 201727902291
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1