Strain identifier

BacDive ID: 133325

Type strain: Yes

Species: Roseovarius aestuariivivens

Strain Designation: GHTF-24

NCBI tax ID(s): 1888910 (species)

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General

@ref: 25160

BacDive-ID: 133325

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming

description: Roseovarius aestuariivivens GHTF-24 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from tidal flat sediment.

NCBI tax id

  • NCBI tax id: 1888910
  • Matching level: species

doi: 10.13145/bacdive133325.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Rhodobacterales
  • family: Roseobacteraceae
  • genus: Roseovarius
  • species: Roseovarius aestuariivivens
  • full scientific name: Roseovarius aestuariivivens Park et al. 2017

@ref: 25160

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhodobacterales

family: Rhodobacteraceae

genus: Roseovarius

species: Roseovarius aestuariivivens

strain designation: GHTF-24

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
25160negative0.4-5.0 µm0.4-1.0 µmcoccus-shapedno
25160ovoid-shaped
25160rod-shaped

colony morphology

  • @ref: 25160
  • colony size: 0.5-1.0 mm
  • colony color: greyish-yellow
  • colony shape: circular
  • incubation period: 5 days
  • medium used: marine agar (MA)

Culture and growth conditions

culture medium

  • @ref: 25160
  • name: marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
25160positiveoptimum30mesophilic
25160positivegrowth10psychrophilic
25160positivegrowth40thermophilic
25160nogrowth4psychrophilic
25160nogrowth45thermophilic

culture pH

@refabilitytypepH
25160positiveoptimum7.0-8.0
25160positivegrowth5.5
25160nogrowth5

Physiology and metabolism

oxygen tolerance

  • @ref: 25160
  • oxygen tolerance: aerobe

halophily

@refsalttested relationconcentrationgrowth
25160NaCloptimum2.0-3.0 %(w/v)
25160NaClgrowth0.0-10.0 %(w/v)positive

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
25160casein-hydrolysis
25160esculin-hydrolysis4853
25160gelatin-hydrolysis5291
25160hypoxanthine-hydrolysis17368
25160L-tyrosine-hydrolysis17895
25160nitrate-reduction17632
25160starch-hydrolysis28017
25160urea-hydrolysis16199
25160xanthine-hydrolysis15318
25160tween 80+hydrolysis53426

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.
2516028971ampicillinyesyes10 µg (disc)
251607507neomycinyesyes30 µg (disc)
2516016869oleandomycinyesyes15 µg (disc)
2516018208penicillin gyesyes20 Unit
2516017076streptomycinyesyes50 µg (disc)
2516017833gentamicinyesno30 µg (disc)
251603393carbenicillinyesyes100 µg (disc)
25160124991cefalotinyesyes30 µg (disc)
2516017698chloramphenicolyesyes100 µg (disc)
251606104kanamycinyesyes30 µg (disc)
251606472lincomycinyesno15 µg (disc)
2516028368novobiocinyesno5 µg (disc)
251608309polymyxin byesno100 Unit
2516027902tetracyclineyesno30 µg (disc)

metabolite production

  • @ref: 25160
  • Chebi-ID: 30033
  • metabolite: bacteriochlorophyll alpha
  • production: no

enzymes

@refvalueactivityec
25160acid phosphatase-3.1.3.2
25160alkaline phosphatase-3.1.3.1
25160alpha-chymotrypsin-3.4.21.1
25160alpha-fucosidase-3.2.1.51
25160alpha-galactosidase-3.2.1.22
25160alpha-glucosidase-3.2.1.20
25160alpha-mannosidase-3.2.1.24
25160beta-galactosidase-3.2.1.23
25160beta-glucosidase-3.2.1.21
25160beta-glucuronidase-3.2.1.31
25160catalase+1.11.1.6
25160cystine arylamidase-3.4.11.3
25160cytochrome oxidase+1.9.3.1
25160esterase (C 4)+
25160esterase Lipase (C 8)+
25160leucine arylamidase+3.4.11.1
25160lipase (C 14)-
25160N-acetyl-beta-glucosaminidase-3.2.1.52
25160naphthol-AS-BI-phosphohydrolase+
25160trypsin-3.4.21.4
25160valine arylamidase-

fatty acid profile

  • @reffatty acidpercentageECL
    2516011-Methyl C18:1ω7c4.9
    25160C12:03.5
    25160C12:0 3OH2.1
    25160C16:09.4
    25160C18:02.1
    25160C18:1ω7c73.7
    25160C19:1ω6c / cyclo C19:0ω10c1.619
    25160Cyclo C19:0ω8c0.9
    25160C15:1 iso F0.6
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine Agar (MA)
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 3
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA 6
  • system: MIS MIDI
  • @reffatty acidpercentageECL
    2516011-Methyl C18:1ω7c6.1
    25160C12:03
    25160C12:0 3OH2.3
    25160C16:09.4
    25160C16:1ω7c / C16:1ω6c0.6
    25160C18:02.6
    25160C18:1ω7c72.9
    25160C19:1ω6c / cyclo C19:0ω10c1.419
    25160Cyclo C19:0ω8c1
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine Agar (MA)
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 5
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA 6
  • system: MIS MIDI
  • @reffatty acidpercentageECL
    2516011-Methyl C18:1ω7c6.7
    25160C12:03.7
    25160C12:0 3OH2.5
    25160C16:011.2
    25160C16:1ω7c / C16:1ω6c1.1
    25160C18:01.9
    25160C18:1ω7c67.5
    25160C19:1ω6c / cyclo C19:0ω10c1.819
    25160Cyclo C19:0ω8c2.4
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine Agar (MA)
  • agar/liquid: agar
  • incubation temperature: 30
  • incubation time: 7
  • software version: Sherlock 6.2B
  • library/peak naming table: TSBA 6
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

  • @ref: 25160
  • sample type: tidal flat sediment
  • geographic location: Goheung on the South Sea
  • country: Republic of Korea
  • origin.country: KOR
  • continent: Asia
  • enrichment culture: marine agar 2216 (MA; BD Difco)
  • enrichment culture temperature: 25
  • isolation procedure: standard dilution plating technique

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Tidal flat
#Environmental#Terrestrial#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_62126.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_19;96_1722;97_14365;98_18563;99_62126&stattab=map
  • Last taxonomy: Roseovarius aestuariivivens subclade
  • 16S sequence: KX641473
  • Sequence Identity:
  • Total samples: 933
  • soil counts: 21
  • aquatic counts: 861
  • animal counts: 42
  • plant counts: 9

Sequence information

16S sequences

  • @ref: 25160
  • description: Roseovarius sp. GHTF-24 16S ribosomal RNA gene, partial sequence
  • accession: KX641473
  • length: 1424
  • database: nuccore
  • NCBI tax ID: 1888910

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Roseovarius aestuariivivens GHTF-24GCA_004761875contigncbi1888910
66792Roseovarius aestuariivivens GHTF-242895604787draftimg1888910

GC content

  • @ref: 25160
  • GC-content: 62.3
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 25160

culture collection no.: KCTC 52454, NBRC 112420

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902178
  • title: Roseovarius aestuariivivens sp. nov., isolated from a tidal flat.
  • authors: Park S, Ha MJ, Yoon SY, Jung YT, Yoon JH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001562
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Rhodobacteraceae/*classification/genetics/isolation & purification, Seawater/*microbiology, Sequence Analysis, DNA, Ubiquinone/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25160S. H. Park, M. J.,Yoon, S. Y.,Jung, Y. T.,Yoon, J. H.Roseovarius aestuariivivens sp. nov., isolated from a tidal flat10.1099/ijsem.0.001562IJSEM 67: 25-30 201727902178
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/