Strain identifier

BacDive ID: 133316

Type strain: Yes

Species: Nonlabens halophilus

Strain history: <- Wonyong Kim, Chung-Ang Univ..

NCBI tax ID(s): 1854709 (species)

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General

@ref: 25158

BacDive-ID: 133316

keywords: 16S sequence, Bacteria, anaerobe, colony-forming

description: Nonlabens halophilus KCTC 52177 is an anaerobe bacterium that forms circular colonies and was isolated from reclaimed land collected from Modo in the Republic of Korea.

NCBI tax id

  • NCBI tax id: 1854709
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- Wonyong Kim, Chung-Ang Univ..

doi: 10.13145/bacdive133316.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Nonlabens
  • species: Nonlabens halophilus
  • full scientific name: Nonlabens halophilus Oh et al. 2017

@ref: 25158

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Nonlabens

species: Nonlabens halophilus

type strain: yes

Morphology

colony morphology

  • @ref: 25158
  • colony color: orange
  • colony shape: circular
  • incubation period: 3 days
  • medium used: MA

Culture and growth conditions

culture medium

  • @ref: 25158
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
25158positivegrowth10-37
25158positiveoptimum30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
25158positivegrowth5.5-10.5alkaliphile
25158positiveoptimum6.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
25158anaerobe
67771aerobe

spore formation

  • @ref: 25158
  • spore formation: no

halophily

@refsalttested relationconcentrationgrowth
25158NaCloptimum2 %
25158NaClgrowth0-5 %(w/v)positive

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
25158168082-dehydro-D-gluconate-assimilation
25158581435-dehydro-D-gluconate-assimilation
2515817128adipate-assimilation
2515815963ribitol-assimilation
2515818333D-arabitol-assimilation
2515828847D-fucose-assimilation
2515817634D-glucose-carbon source
2515816024D-mannose-assimilation
251586731melezitose-assimilation
2515817113erythritol-assimilation
2515816813galactitol-assimilation
2515830849L-arabinose-assimilation
2515818403L-arabitol-assimilation
2515818287L-fucose-assimilation
2515817266L-sorbose-assimilation
2515865328L-xylose-assimilation
2515825115malate-assimilation
2515817306maltose-assimilation
2515874863methyl beta-D-xylopyranoside-assimilation
25158506227N-acetylglucosamine-assimilation
2515818401phenylacetate-assimilation
2515853258sodium citrate-assimilation
2515817992sucrose-builds acid from
2515817992sucrose-carbon source
2515817151xylitol-assimilation
2515862318D-lyxose+builds acid from
2515862318D-lyxose+carbon source
2515816899D-mannitol+assimilation
2515816988D-ribose+carbon source
2515817924D-sorbitol+assimilation
2515816443D-tagatose+builds acid from
2515865327D-xylose+carbon source
251584853esculin+hydrolysis
251585291gelatin+hydrolysis
2515817268myo-inositol+assimilation
2515862345L-rhamnose+assimilation

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivesensitivity conc.group ID
251582676amoxicillinyesyes20 µg (disc)
2515828971ampicillinyesyes10 µg (disc)
251583393carbenicillinyesyes100 µg (disc)
25158209807cefoxitinyesyes30 µg (disc)
25158124991cefalotinyesyes30 µg (disc)
2515817698chloramphenicolyesyes30 µg (disc)
2515848923erythromycinyesyes15 µg (disc)
2515817833gentamicinyesyes10 µg (disc)
251586104kanamycinyesyes30 µg (disc)
2515817334penicillinyesyes10 Unit
25158100147nalidixic acidyesyes30 µg (disc)
251588309polymyxin byesyes300 Unit
2515828077rifampicinyesyes5 µg (disc)
2515817076streptomycinyesyes10 µg (disc)
2515827902tetracyclineyesyes30 µg (disc)
2515828864tobramycinyesyes10 µg (disc)
2515845924trimethoprimyesyes1.25 µg (disc)26
251589332sulfamethoxazoleyesyes23.75 µg (disc)26

enzymes

@refvalueactivityec
25158acid phosphatase+3.1.3.2
25158alkaline phosphatase+3.1.3.1
25158alpha-chymotrypsin-3.4.21.1
25158alpha-fucosidase-3.2.1.51
25158alpha-galactosidase-3.2.1.22
25158alpha-glucosidase-3.2.1.20
25158alpha-mannosidase-3.2.1.24
25158beta-galactosidase-3.2.1.23
25158beta-glucosidase-3.2.1.21
25158beta-glucuronidase-3.2.1.31
25158catalase+1.11.1.6
25158cystine arylamidase-3.4.11.3
25158cytochrome oxidase+1.9.3.1
25158esterase (C 4)+
25158esterase Lipase (C 8)+
25158leucine arylamidase-3.4.11.1
25158lipase (C 14)+
25158N-acetyl-beta-glucosaminidase-3.2.1.52
25158naphthol-AS-BI-phosphohydrolase+
25158trypsin-3.4.21.4
25158valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25158C15:0 anteiso5.67
    25158C17:1 anteiso ω9c3.84
    25158C15:0 2OH2.99
    25158C15:1ω6c1.31
    25158C16:01.05
    25158C16:0 3OH2.63
    25158C16:1ω7c / C16:1ω6c17.71
    25158C17:0 2OH5.27
    25158C17:1ω6c2.7
    25158C15:0 iso14.12
    25158C15:0 iso 3OH7.3
    25158C16:0 iso1.55
    25158C16:0 iso 3OH4.56
    25158C16:1 iso H1.38
    25158C17:0 iso 3OH17.84
    25158C17:1 iso ω9c4.55
  • type of FA analysis: whole cell analysis
  • incubation medium: Marine Agar (MA)
  • agar/liquid: agar
  • incubation temperature: 37
  • incubation time: 3
  • software version: Sherlock 6.1
  • system: MIS MIDI
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
25158reclaimed land collected from Modo in the Republic of KoreaModo-ri, Uisin-myeon,Jindo, Jeollanam-do, South KoreaRepublic of KoreaKORAsia34.406126.355
67771From soil of ModoRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial

taxonmaps

  • @ref: 69479
  • File name: preview.99_85824.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_420;96_35739;97_45402;98_59425;99_85824&stattab=map
  • Last taxonomy: Nonlabens halophilus subclade
  • 16S sequence: KU719509
  • Sequence Identity:
  • Total samples: 180
  • soil counts: 5
  • aquatic counts: 169
  • animal counts: 6

Sequence information

16S sequences

  • @ref: 25158
  • description: 16S rRNA gene sequence
  • accession: KU719509
  • length: 1504
  • database: ena
  • NCBI tax ID: 1854709

GC content

  • @ref: 25158
  • GC-content: 38.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 25158

culture collection no.: KCTC 52177, NBRC 111996, CAU 1131

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27902210Nonlabens halophilus sp. nov., isolated from reclaimed land.Oh M, Kim JH, Bora N, Kim WInt J Syst Evol Microbiol10.1099/ijsem.0.0015902017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacteriaceae/*classification/genetics/isolation & purification, Glycolipids/chemistry, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny30941530Nonlabens xiamenensis sp. nov., isolated from coastal seawater.Huang Z, Du Y, Lai Q, Shao ZAntonie Van Leeuwenhoek10.1007/s10482-019-01258-82019Bacterial Typing Techniques, Base Composition, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, Flavobacteriaceae/*classification/genetics/*isolation & purification/physiology, Phospholipids/analysis, Phylogeny, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNATranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25158M. K. Oh, J. H.,Bora, N.,Kim, W.Nonlabens halophilus sp. nov., isolated from reclaimed land10.1099/ijsem.0.001590IJSEM 67: 138-143 201727902210
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/