Strain identifier
BacDive ID: 133311
Type strain:
Species: Flavobacterium orientale
Strain Designation: SP3
NCBI tax ID(s): 1756020 (species)
version 8.1 (current version)
General
@ref: 25148
BacDive-ID: 133311
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, Gram-negative, colony-forming
description: Flavobacterium orientale SP3 is an obligate aerobe, Gram-negative bacterium that forms circular colonies and was isolated from water of Sayram Lake, in Xinjiang.
NCBI tax id
- NCBI tax id: 1756020
- Matching level: species
doi: 10.13145/bacdive133311.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacteroidota
- domain: Bacteria
- phylum: Bacteroidota
- class: Flavobacteriia
- order: Flavobacteriales
- family: Flavobacteriaceae
- genus: Flavobacterium
- species: Flavobacterium orientale
- full scientific name: Flavobacterium orientale Li et al. 2017
@ref: 25148
domain: Bacteria
phylum: Bacteroidetes
class: Flavobacteriia
order: Flavobacteriales
family: Flavobacteriaceae
genus: Flavobacterium
species: Flavobacterium orientale
strain designation: SP3
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.999
colony morphology
- @ref: 25148
- colony size: 2.0-3.0 mm
- colony color: orange
- colony shape: circular
- incubation period: 3 days
- medium used: R2A
pigmentation
- @ref: 25148
- production: no
- name: Flexirubin-type
Culture and growth conditions
culture medium
@ref | name | growth |
---|---|---|
25148 | Reasoner's 2A agar (R2A) | yes |
25148 | NA (Nutrient agar) | yes |
25148 | Trypticase Soy Agar (TSA) | yes |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
25148 | positive | growth | 4-30 | |
25148 | positive | optimum | 25 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
25148 | positive | growth | 6.5-9.5 | alkaliphile |
25148 | positive | optimum | 7.0-8.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 25148
- oxygen tolerance: obligate aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.986 |
halophily
@ref | salt | tested relation | concentration | growth |
---|---|---|---|---|
25148 | NaCl | optimum | 0.5-1.0 % | |
25148 | NaCl | growth | 0-2.0 %(w/v) | positive |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
25148 | 15588 | D-malate | - | oxidation |
25148 | 30916 | 2-oxoglutarate | - | builds acid from |
25148 | 73918 | 3-O-methyl-D-glucose | - | oxidation |
25148 | 27613 | amygdalin | - | builds acid from |
25148 | 18305 | arbutin | - | builds acid from |
25148 | 85146 | carboxymethylcellulose | - | hydrolysis |
25148 | 17057 | cellobiose | - | builds acid from |
25148 | 17057 | cellobiose | - | oxidation |
25148 | 17029 | chitin | - | hydrolysis |
25148 | 16947 | citrate | - | assimilation |
25148 | 16947 | citrate | - | oxidation |
25148 | 15963 | ribitol | - | builds acid from |
25148 | 17108 | D-arabinose | - | builds acid from |
25148 | 18333 | D-arabitol | - | builds acid from |
25148 | 15824 | D-fructose | - | builds acid from |
25148 | 15824 | D-fructose | - | oxidation |
25148 | 28847 | D-fucose | - | builds acid from |
25148 | 28847 | D-fucose | - | oxidation |
25148 | 12936 | D-galactose | - | builds acid from |
25148 | 17634 | D-glucose | - | builds acid from |
25148 | 17716 | lactose | - | builds acid from |
25148 | 62318 | D-lyxose | - | builds acid from |
25148 | 16024 | D-mannose | - | builds acid from |
25148 | 16024 | D-mannose | - | oxidation |
25148 | 16988 | D-ribose | - | builds acid from |
25148 | 16523 | D-serine | - | oxidation |
25148 | 17924 | D-sorbitol | - | oxidation |
25148 | 16443 | D-tagatose | - | builds acid from |
25148 | 65327 | D-xylose | - | builds acid from |
25148 | 16991 | dna | - | hydrolysis |
25148 | egg yolk | - | hydrolysis | |
25148 | 17113 | erythritol | - | builds acid from |
25148 | 16813 | galactitol | - | builds acid from |
25148 | 28066 | gentiobiose | - | oxidation |
25148 | 24265 | gluconate | - | builds acid from |
25148 | 17234 | glucose | - | fermentation |
25148 | 17754 | glycerol | - | builds acid from |
25148 | 17754 | glycerol | - | oxidation |
25148 | 13643 | glycol | - | builds acid from |
25148 | 17596 | inosine | - | oxidation |
25148 | 17268 | myo-inositol | - | builds acid from |
25148 | 17268 | myo-inositol | - | oxidation |
25148 | 30849 | L-arabinose | - | builds acid from |
25148 | 18403 | L-arabitol | - | builds acid from |
25148 | 18287 | L-fucose | - | builds acid from |
25148 | 18287 | L-fucose | - | oxidation |
25148 | 62345 | L-rhamnose | - | builds acid from |
25148 | 62345 | L-rhamnose | - | oxidation |
25148 | 17266 | L-sorbose | - | builds acid from |
25148 | 65328 | L-xylose | - | builds acid from |
25148 | 24996 | lactate | - | oxidation |
25148 | 6731 | melezitose | - | builds acid from |
25148 | 28053 | melibiose | - | builds acid from |
25148 | 28053 | melibiose | - | oxidation |
25148 | 320061 | methyl alpha-D-glucopyranoside | - | builds acid from |
25148 | 43943 | methyl alpha-D-mannoside | - | builds acid from |
25148 | 74863 | methyl beta-D-xylopyranoside | - | builds acid from |
25148 | 63154 | N-acetyl-beta-D-mannosamine | - | oxidation |
25148 | 506227 | N-acetylglucosamine | - | builds acid from |
25148 | 506227 | N-acetylglucosamine | - | oxidation |
25148 | 17632 | nitrate | - | reduction |
25148 | 16634 | raffinose | - | builds acid from |
25148 | 16634 | raffinose | - | oxidation |
25148 | 17814 | salicin | - | builds acid from |
25148 | 17814 | salicin | - | oxidation |
25148 | 30911 | sorbitol | - | builds acid from |
25148 | 17164 | stachyose | - | oxidation |
25148 | 17992 | sucrose | - | builds acid from |
25148 | 17992 | sucrose | - | oxidation |
25148 | synanthrin | - | builds acid from | |
25148 | 27082 | trehalose | - | builds acid from |
25148 | 27082 | trehalose | - | oxidation |
25148 | 32528 | turanose | - | builds acid from |
25148 | 32528 | turanose | - | oxidation |
25148 | 53424 | tween 20 | - | hydrolysis |
25148 | 53426 | tween 80 | - | hydrolysis |
25148 | 18186 | tyrosine | - | hydrolysis |
25148 | 17151 | xylitol | - | builds acid from |
25148 | 30089 | acetate | + | oxidation |
25148 | 13705 | acetoacetate | + | oxidation |
25148 | 17925 | alpha-D-glucose | + | oxidation |
25148 | 18333 | D-arabitol | + | oxidation |
25148 | 18024 | D-galacturonic acid | + | oxidation |
25148 | 23652 | dextrin | + | oxidation |
25148 | 4853 | esculin | + | hydrolysis |
25148 | 4853 | esculin | + | builds acid from |
25148 | 5291 | gelatin | + | hydrolysis |
25148 | 5291 | gelatin | + | oxidation |
25148 | 70744 | glycine-proline | + | oxidation |
25148 | 28087 | glycogen | + | builds acid from |
25148 | 16467 | L-arginine | + | oxidation |
25148 | 29991 | L-aspartate | + | oxidation |
25148 | 17464 | L-galactonic acid gamma-lactone | + | oxidation |
25148 | 29985 | L-glutamate | + | oxidation |
25148 | 15589 | L-malate | + | oxidation |
25148 | 17306 | maltose | + | builds acid from |
25148 | 17306 | maltose | + | oxidation |
25148 | 51850 | methyl pyruvate | + | oxidation |
25148 | 28037 | N-acetylgalactosamine | + | oxidation |
25148 | 17309 | pectin | + | oxidation |
25148 | 17272 | propionate | + | oxidation |
25148 | 28017 | starch | + | hydrolysis |
25148 | 28017 | starch | + | builds acid from |
25148 | 53423 | tween 40 | + | oxidation |
25148 | 53425 | tween 60 | + | hydrolysis |
25148 | 58143 | 5-dehydro-D-gluconate | +/- | builds acid from |
25148 | casein | +/- | hydrolysis | |
25148 | 12936 | D-galactose | +/- | oxidation |
25148 | 14314 | D-glucose 6-phosphate | +/- | oxidation |
25148 | 16024 | D-mannose | +/- | oxidation |
25148 | 28066 | gentiobiose | +/- | builds acid from |
25148 | 17115 | L-serine | +/- | oxidation |
25148 | 74611 | methyl (R)-lactate | +/- | oxidation |
25148 | 35418 | n-acetylneuraminate | +/- | oxidation |
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is resistant | resistance conc. | is sensitive | sensitivity conc. |
---|---|---|---|---|---|---|---|
25148 | 31810 | fleroxacin | yes | yes | 5 µg | ||
25148 | 100241 | ciprofloxacin | yes | yes | 5 µg | ||
25148 | 9332 | sulfamethoxazole | yes | yes | 1.25 µg | ||
25148 | 8232 | piperacillin | yes | yes | 100 µg | ||
25148 | 7809 | oxacillin | yes | yes | 1 µg | ||
25148 | 71415 | nitrofurantoin | yes | yes | 300 µg | ||
25148 | 116278 | lomefloxacin | yes | yes | 10 µg | ||
25148 | 18208 | penicillin g | yes | yes | 10 | ||
25148 | 48923 | erythromycin | yes | yes | 15 µg | ||
25148 | 17698 | chloramphenicol | yes | yes | 30 µg | ||
25148 | 2955 | azithromycin | yes | yes | 15 µg | ||
25148 | 3745 | clindamycin | yes | yes | 2 µg | ||
25148 | 50845 | doxycycline | yes | yes | 30 µg | ||
25148 | 3732 | clarithromycin | yes | yes | 15 µg | ||
25148 | 28001 | vancomycin | yes | yes | 30 µg | ||
25148 | 7528 | netilmycin | yes | yes | 30 µg | ||
25148 | 29007 | ceftriaxone | yes | yes | 30 µg | ||
25148 | 3478 | cefaclor | yes | yes | 30 µg | ||
25148 | 474053 | cefazolin | yes | yes | 30 µg | ||
25148 | 204928 | cefotaxime | yes | yes | 30 µg | ||
25148 | 28971 | ampicillin | yes | yes | 10 µg | ||
25148 | 50694 | minocycline | yes | yes | 30 µg | ||
25148 | 28077 | rifampicin | yes | yes | 5 µg | ||
25148 | 27902 | tetracycline | yes | yes | 30 µg | ||
25148 | 2637 | amikacin | yes | yes | 30 µg | ||
25148 | 3508 | ceftazidime | yes | yes | 30 µg | ||
25148 | 124991 | cefalotin | yes | yes | 30 µg | ||
25148 | 3493 | cefoperazone | yes | yes | 75 µg | ||
25148 | 3517 | cefuroxime | yes | yes | 30 µg |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
25148 | 16136 | hydrogen sulfide | no |
25148 | 35581 | indole | no |
enzymes
@ref | value | activity | ec |
---|---|---|---|
25148 | acid phosphatase | + | 3.1.3.2 |
25148 | alkaline phosphatase | + | 3.1.3.1 |
25148 | alpha-chymotrypsin | + | 3.4.21.1 |
25148 | alpha-fucosidase | - | 3.2.1.51 |
25148 | alpha-galactosidase | - | 3.2.1.22 |
25148 | alpha-glucosidase | +/- | 3.2.1.20 |
25148 | alpha-mannosidase | - | 3.2.1.24 |
25148 | beta-galactosidase | - | 3.2.1.23 |
25148 | beta-glucosidase | - | 3.2.1.21 |
25148 | beta-glucuronidase | - | 3.2.1.31 |
25148 | catalase | + | 1.11.1.6 |
25148 | cystine arylamidase | + | 3.4.11.3 |
25148 | cytochrome oxidase | + | 1.9.3.1 |
25148 | esterase (C 4) | + | |
25148 | esterase Lipase (C 8) | + | |
25148 | leucine arylamidase | + | 3.4.11.1 |
25148 | lipase (C 14) | - | |
25148 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
25148 | naphthol-AS-BI-phosphohydrolase | + | |
25148 | trypsin | + | 3.4.21.4 |
25148 | urease | - | 3.5.1.5 |
25148 | valine arylamidase | + |
fatty acid profile
fatty acids
@ref fatty acid percentage 25148 C15:0 anteiso 1.8 25148 C15:1 anteiso A 0.7 25148 C17:1 anteiso ω9c 0.4 25148 C14:0 0.3 25148 C15:0 2OH 0.3 25148 C15:1ω6c 3.5 25148 C16:0 0.7 25148 C16:0 3OH 0.4 25148 C16:1ω7c / C16:1ω6c 3.6 25148 C17:0 2OH 0.4 25148 C17:1ω6c 1.9 25148 C17:1ω8c 0.6 25148 C18:1ω5c 0.3 25148 C13:0 iso 0.5 25148 C14:0 iso 0.7 25148 C15:0 iso 33 25148 C15:0 iso 3OH 6.2 25148 C15:1 iso G 19.1 25148 C16:0 iso 1.6 25148 C16:0 iso 3OH 1.6 25148 C16:1 iso H 1.1 25148 C17:0 iso 0.4 25148 C17:0 iso 3OH 7.9 25148 iso-C17:1 I / anteiso-C17:1 1.1 25148 C17:1 iso ω9c / C16:0 10-methyl 10.7 - type of FA analysis: whole cell analysis
- incubation medium: Tryptic soy agar (TSA)
- agar/liquid: agar
- incubation temperature: 25
- incubation time: 3
- software version: Sherlock 6.0
- library/peak naming table: TSBA 6
- system: MIS MIDI
- method/protocol: Sasser 1990
Isolation, sampling and environmental information
isolation
- @ref: 25148
- sample type: water of Sayram Lake, in Xinjiang
- geographic location: Sayram Lake, Xinjiang
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
- Cat3: #Lake (large)
Sequence information
16S sequences
- @ref: 25148
- description: 16S rRNA gene sequence
- accession: KT985058
- length: 1477
- database: ena
- NCBI tax ID: 1756020
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Flavobacterium orientale CGMCC 1.12506 | GCA_014638005 | scaffold | ncbi | 1756020 |
66792 | Flavobacterium orientale strain CGMCC 1.12506 | 1756020.3 | wgs | patric | 1756020 |
GC content
- @ref: 25148
- GC-content: 34.9
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
flagellated | no | 95.136 | no |
gram-positive | no | 99.041 | no |
anaerobic | no | 98.582 | yes |
aerobic | yes | 84.478 | no |
halophile | no | 85.991 | no |
spore-forming | no | 96.462 | no |
thermophile | no | 98.56 | yes |
glucose-util | yes | 87.601 | no |
motile | no | 87.443 | no |
glucose-ferment | no | 89.843 | yes |
External links
@ref: 25148
culture collection no.: CGMCC 1.12506, NBRC 109717
literature
- topic: Phylogeny
- Pubmed-ID: 27902194
- title: Flavobacterium orientale sp. nov., isolated from lake water.
- authors: Li AH, Liu HC, Zhou YG
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001544
- year: 2017
- mesh: Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Lakes/*microbiology, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
25148 | A. H. L. Li, H. C.,Zhou, Y. G. | Flavobacterium orientale sp. nov., isolated from lake water | 10.1099/ijsem.0.001544 | IJSEM 67: 108-112 2017 | 27902194 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 |