Strain identifier

BacDive ID: 133310

Type strain: Yes

Species: Flavobacterium inkyongense

Strain history: <- Jang-Cheon Cho, Inha Univ.

NCBI tax ID(s): 1960096 (species)

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General

@ref: 25147

BacDive-ID: 133310

keywords: 16S sequence, Bacteria, aerobe, rod-shaped, colony-forming

description: Flavobacterium inkyongense KCTC 52244 is an aerobe, rod-shaped bacterium that forms circular colonies and was isolated from an artificial freshwater pond .

NCBI tax id

  • NCBI tax id: 1960096
  • Matching level: species

strain history

@refhistory
67770J.-C. Cho; Inha Univ., South Korea; IMCC27201.
67771<- Jang-Cheon Cho, Inha Univ.

doi: 10.13145/bacdive133310.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Flavobacterium
  • species: Flavobacterium inkyongense
  • full scientific name: Flavobacterium inkyongense Park et al. 2017

@ref: 25147

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Flavobacterium

species: Flavobacterium inkyongense

type strain: yes

Morphology

cell morphology

@refcell shapemotilitygram stain
67771rod-shapedno
67771negative

colony morphology

  • @ref: 25147
  • colony color: yellow
  • colony shape: circular
  • incubation period: 3 days
  • medium used: R2A

pigmentation

  • @ref: 25147
  • production: no
  • name: Flexirubin-type

Culture and growth conditions

culture medium

@refnamegrowth
25147Reasoner's 2A agar (R2A)yes
25147Marine agar (MA)no
25147Trypticase Soy Agar (TSA)no
25147NA (Nutrient agar)yes

culture temp

@refgrowthtypetemperaturerange
25147positivegrowth10-37
25147positiveoptimum30mesophilic
67770positivegrowth30mesophilic
67771positivegrowth30mesophilic

culture pH

@refabilitytypepH
25147positivegrowth7.0-8.0
25147positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
25147aerobe
67771aerobe

halophily

@refsalttested relationconcentrationgrowth
25147NaCloptimum0 %
25147NaClgrowth0-0.25 %(w/v)positive

observation

@refobservation
67770quinones: MK-6
67771quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2514785146carboxymethylcellulose-hydrolysis
2514717029chitin-hydrolysis
2514716991dna-hydrolysis
2514717234glucose-fermentation
2514717632nitrate-reduction
2514753424tween 20-hydrolysis
25147309162-oxoglutarate+respiration
25147casein+hydrolysis
2514712936D-galactose+respiration
251474853esculin+hydrolysis
2514729985L-glutamate+respiration
2514717203L-proline+respiration
2514717306maltose+respiration
2514728017starch+hydrolysis
2514753423tween 40+respiration
2514753426tween 80+hydrolysis
2514753426tween 80+respiration

metabolite production

@refChebi-IDmetaboliteproduction
2514716136hydrogen sulfideno
2514735581indoleno

enzymes

@refvalueactivityec
25147acid phosphatase+3.1.3.2
25147alkaline phosphatase+3.1.3.1
25147alpha-chymotrypsin-3.4.21.1
25147alpha-fucosidase-3.2.1.51
25147alpha-galactosidase-3.2.1.22
25147alpha-glucosidase-3.2.1.20
25147alpha-mannosidase-3.2.1.24
25147arginine dihydrolase-3.5.3.6
25147beta-galactosidase-3.2.1.23
25147beta-glucosidase-3.2.1.21
25147beta-glucuronidase-3.2.1.31
25147catalase-1.11.1.6
25147cystine arylamidase-3.4.11.3
25147cytochrome oxidase+1.9.3.1
25147esterase (C 4)+
25147esterase Lipase (C 8)+
25147gelatinase+
25147leucine arylamidase+3.4.11.1
25147lipase (C 14)-
25147N-acetyl-beta-glucosaminidase-3.2.1.52
25147naphthol-AS-BI-phosphohydrolase+
25147trypsin+3.4.21.4
25147urease+3.5.1.5
25147valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    2514710-Methyl C16:0 / iso-C17:1ω9c3.3
    25147C15:0 2OH1.4
    25147C15:0 3OH2.7
    25147C15:1ω6c1.3
    25147C16:01.1
    25147C16:1ω7c / C16:1ω6c6.2
    25147C12:0 iso1.1
    25147C13:0 iso3.2
    25147C14:0 iso3.4
    25147C15:0 iso31.1
    25147C15:0 iso 3OH9.5
    25147C15:1 iso G17.6
    25147C16:0 iso1.3
    25147C16:0 iso 3OH1.9
    25147C17:0 iso 3OH6.7
  • type of FA analysis: whole cell analysis
  • incubation medium: R2A
  • agar/liquid: agar
  • incubation temperature: 25
  • incubation time: 3
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA 6
  • system: MIS MIDI
  • method/protocol: Sasser 1990
  • cutoff value:

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
25147an artificial freshwater pond (Inkyong Pond)Inkyong Pond, IncheonRepublic of KoreaKORAsia
67770Artificial freshwater pond (Inkyong)Republic of KoreaKORAsia
67771From freshwater, artificial pond(man-made lakes, Inkyungho)Inha University, Nam-gu, IncheonRepublic of KoreaKORAsia37.4497126.656

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Engineered
#Environmental#Aquatic#Pond (small)

Sequence information

16S sequences

  • @ref: 25147
  • description: 16S rRNA gene sequence
  • accession: KX025140
  • length: 1421
  • database: ena
  • NCBI tax ID: 1960096

GC content

@refGC-contentmethod
2514732.1Thermal denaturation, fluorometry
6777032.1thermal denaturation, midpoint method (Tm)
6777132.1

External links

@ref: 25147

culture collection no.: KCTC 52244, JCM 31385, IMCC 27201, BCRC 81043

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27902204Flavobacterium inkyongense sp. nov., isolated from an artificial freshwater pond.Park M, Joung Y, Nam GG, Kim S, Cho JCInt J Syst Evol Microbiol10.1099/ijsem.0.0015752017Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Fresh Water/microbiology, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, Ponds/*microbiology, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny31758394Flavobacterium humi sp. nov., a flexirubin-type pigment producing bacterium, isolated from soil.Kim I, Kim J, Chhetri G, Seo TJ Microbiol10.1007/s12275-019-9350-x2019Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Flavobacterium/*classification/genetics/*isolation & purification/*metabolism, Nucleic Acid Hybridization, Phosphatidylethanolamines/analysis, *Phylogeny, Pigments, Biological/*metabolism, Polyenes/*metabolism, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Soil, *Soil Microbiology, Vitamin K 2/analogs & derivatives/analysisMetabolism

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25147M. J. Park, Y.,Nam, G. G.,Kim, S.,Cho, J. C.Flavobacterium inkyongense sp. nov., isolated from an artificial freshwater pond10.1099/ijsem.0.001575IJSEM 67: 82-86 201727902204
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc