Strain identifier

BacDive ID: 133280

Type strain: Yes

Species: Novosphingobium bradum

Strain Designation: STM-24

NCBI tax ID(s): 1737444 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 8.1 (current version)

General

@ref: 25119

BacDive-ID: 133280

keywords: 16S sequence, Bacteria, aerobe, chemoheterotroph, Gram-negative, rod-shaped, colony-forming

description: Novosphingobium bradum STM-24 is an aerobe, chemoheterotroph, Gram-negative bacterium that forms circular colonies and was isolated from spring.

NCBI tax id

  • NCBI tax id: 1737444
  • Matching level: species

doi: 10.13145/bacdive133280.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Sphingomonadales
  • family: Erythrobacteraceae
  • genus: Novosphingobium
  • species: Novosphingobium bradum
  • full scientific name: Novosphingobium bradum Sheu et al. 2016

@ref: 25119

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Sphingomonadales

family: Sphingomonadaceae

genus: Novosphingobium

species: Novosphingobium bradum

strain designation: STM-24

type strain: yes

Morphology

cell morphology

  • @ref: 25119
  • gram stain: negative
  • cell length: 0.8-2.0 µm
  • cell width: 0.5-0.8 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 25119
  • colony size: 0.3-0.5 mm
  • colony color: light yellow
  • colony shape: circular
  • medium used: R2A agar (Reasoner's 2A agar)

Culture and growth conditions

culture medium

@refnamegrowth
25119NA (Nutrient Agar)yes
25119Reasoner's 2A agar (R2A)yes

culture temp

@refgrowthtypetemperaturerange
25119positivegrowth15.0-30.0
25119positiveoptimum20psychrophilic

culture pH

@refabilitytypepHPH range
25119positivegrowth5.0-9.0alkaliphile
25119positiveoptimum6

Physiology and metabolism

oxygen tolerance

  • @ref: 25119
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 25119
  • type: chemoheterotroph

spore formation

  • @ref: 25119
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
25119NaClpositivegrowth0.0-0.5 %
25119NaCloptimum0 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2511930089acetate-carbon source
2511917128adipate-carbon source
2511917968butyrate-carbon source
2511985146carboxymethylcellulose-hydrolysis
2511917029chitin-hydrolysis
25119corn oil-hydrolysis
2511915824D-fructose-carbon source
2511912936D-galactose-carbon source
2511916024D-mannose-carbon source
2511916991dna-hydrolysis
251195291gelatin-hydrolysis
2511917234glucose-fermentation
2511930849L-arabinose-carbon source
2511925115malate-carbon source
2511928053melibiose-carbon source
25119506227N-acetylglucosamine-carbon source
2511917632nitrate-reduction
2511928017starch-hydrolysis
2511927897tryptophan-energy source
2511953424tween 20-hydrolysis
2511953423tween 40-hydrolysis
2511953425tween 60-hydrolysis
2511916199urea-hydrolysis
25119casein+hydrolysis
2511916947citrate+carbon source
2511917634D-glucose+carbon source
2511916899D-mannitol+carbon source
2511963150D-rhamnose+carbon source
2511917924D-sorbitol+carbon source
251194853esculin+hydrolysis
2511924265gluconate+carbon source
2511916977L-alanine+carbon source
2511917203L-proline+carbon source
2511962345L-rhamnose+carbon source
2511917306maltose+carbon source
2511917992sucrose+carbon source
2511927082trehalose+carbon source
2511953426tween 80+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitivegroup IDis resistant
2511928077rifampicinyesyes
2511917698chloramphenicolyesyes
2511917833gentamicinyesyes
251196104kanamycinyesyes
2511927902tetracyclineyesyes
2511928368novobiocinyesyes
2511945924trimethoprimyesyes25
25119102130sulfamerazineyesyes25
2511918208penicillin gyesyes
2511928971ampicillinyesyes
2511917076streptomycinyesyes
25119100147nalidixic acidyesyes

metabolite production

  • @ref: 25119
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 25119
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
25119acid phosphatase+3.1.3.2
25119alkaline phosphatase+3.1.3.1
25119alpha-chymotrypsin-3.4.21.1
25119alpha-fucosidase-3.2.1.51
25119alpha-galactosidase-3.2.1.22
25119alpha-glucosidase+3.2.1.20
25119alpha-mannosidase-3.2.1.24
25119arginine dihydrolase-3.5.3.6
25119beta-galactosidase+3.2.1.23
25119beta-glucosidase+3.2.1.21
25119beta-glucuronidase-3.2.1.31
25119catalase-1.11.1.6
25119cystine arylamidase-3.4.11.3
25119cytochrome oxidase+1.9.3.1
25119esterase (C 4)+
25119esterase Lipase (C 8)+
25119leucine arylamidase+3.4.11.1
25119lipase (C 14)+
25119N-acetyl-beta-glucosaminidase-3.2.1.52
25119naphthol-AS-BI-phosphohydrolase+
25119trypsin-3.4.21.4
25119tryptophan deaminase-4.1.99.1
25119urease-3.5.1.5
25119valine arylamidase+

Isolation, sampling and environmental information

isolation

  • @ref: 25119
  • sample type: spring
  • geographic location: vicinity of Miaoli County
  • country: Taiwan
  • origin.country: TWN
  • continent: Asia
  • latitude: 24.56
  • longitude: 120.82

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Spring

Sequence information

16S sequences

  • @ref: 25119
  • description: Novosphingobium sp. STM-24 partial 16S rRNA gene, strain STM-24
  • accession: LN890294
  • length: 1411
  • database: nuccore
  • NCBI tax ID: 1737444

GC content

  • @ref: 25119
  • GC-content: 69.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 25119

culture collection no.: BCRC 80925, LMG 29291, KCTC 42984

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27599476Novosphingobium bradum sp. nov., isolated from a spring.Sheu SY, Liu LP, Chen WMInt J Syst Evol Microbiol10.1099/ijsem.0.0014752016Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hydroxybutyrates/chemistry, Natural Springs/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Polyesters/chemistry, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spermidine/chemistry, Sphingomonadaceae/*classification/genetics/isolation & purification, Taiwan, Ubiquinone/chemistryTranscriptome
Phylogeny33411666Novosphingobium olei sp. nov., with the ability to degrade diesel oil, isolated from oil-contaminated soil and proposal to reclassify Novosphingobium stygium as a later heterotypic synonym of Novosphingobium aromaticivorans.Chaudhary DK, Dahal RH, Kim DU, Kim JInt J Syst Evol Microbiol10.1099/ijsem.0.0046282021Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, *Petroleum Pollution, Phospholipids/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants, Sphingomonadaceae/*classification/isolation & purification, Ubiquinone/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
25119S. Y. L. Sheu, L. P.,Chen, W. M.Novosphingobium bradum sp. nov., isolated from a spring10.1099/ijsem.0.001475IJSEM 66: 5083-5090 201627599476