Strain identifier

BacDive ID: 13322

Type strain: Yes

Species: Prauserella muralis

Strain Designation: 05-Be-005

Strain history: CCM 7635 <-- P. Kämpfer 05-Be-005.

NCBI tax ID(s): 588067 (species)

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General

@ref: 16164

BacDive-ID: 13322

DSM-Number: 45305

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive

description: Prauserella muralis 05-Be-005 is an aerobe, spore-forming, mesophilic bacterium that was isolated from wall colonized with moulds.

NCBI tax id

  • NCBI tax id: 588067
  • Matching level: species

strain history

@refhistory
16164<- P. Kämpfer, Univ. Giessen, Germany; 05-Be-005
67770CCM 7635 <-- P. Kämpfer 05-Be-005.

doi: 10.13145/bacdive13322.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Prauserella
  • species: Prauserella muralis
  • full scientific name: Prauserella muralis Schäfer et al. 2010

@ref: 16164

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Prauserella

species: Prauserella muralis

full scientific name: Prauserella muralis Schäfer et al. 2010

strain designation: 05-Be-005

type strain: yes

Morphology

cell morphology

@refgram staincell widthmotilityconfidence
29384positive1.3 µm
69480no90.167
69480positive100

colony morphology

  • @ref: 61322
  • incubation period: 3-7 days

pigmentation

  • @ref: 29384
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16164TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
16164GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
16164positivegrowth28mesophilic
29384positivegrowth25-30mesophilic
61322positivegrowth28mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
29384aerobe
61322aerobe

spore formation

@refspore formationconfidence
29384yes
69481yes100
69480yes100

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2938422599arabinose+carbon source
2938435391aspartate+carbon source
2938417240itaconate+carbon source
2938424996lactate+carbon source
2938425017leucine+carbon source
2938417306maltose+carbon source
2938437684mannose+carbon source
29384506227N-acetylglucosamine+carbon source
2938418257ornithine+carbon source
2938418401phenylacetate+carbon source
2938417822serine+carbon source
2938430911sorbitol+carbon source
2938427082trehalose+carbon source

enzymes

  • @ref: 29384
  • value: cytochrome oxidase
  • activity: +
  • ec: 1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
16164wall colonized with mouldsBerlinGermanyDEUEurope
61322Wall of an indoor environment colonized with mouldBerlinGermanyDEUEurope
67770Wall of a house colonized with mouldsBerlinGermanyDEUEurope

isolation source categories

Cat1Cat2
#Engineered#Built environment
#Host#Fungi

taxonmaps

  • @ref: 69479
  • File name: preview.99_51706.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_7302;97_29092;98_37117;99_51706&stattab=map
  • Last taxonomy: Prauserella muralis
  • 16S sequence: FM956091
  • Sequence Identity:
  • Total samples: 32
  • soil counts: 26
  • aquatic counts: 1
  • animal counts: 3
  • plant counts: 2

Safety information

risk assessment

  • @ref: 16164
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 16164
  • description: Prauserella sp. 05-Be-005 partial 16S rRNA gene, type strain 05-Be-005T
  • accession: FM956091
  • length: 1412
  • database: ena
  • NCBI tax ID: 588067

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prauserella muralis DSM 45305GCA_007993965contigncbi588067
66792Prauserella muralis strain DSM 45305588067.3wgspatric588067
66792Prauserella muralis strain DSM 45305588067.5wgspatric588067
66792Prauserella muralis DSM 453052818991319draftimg588067
66792Prauserella muralis DSM 453052865233180draftimg588067
67770Prauserella muralis DSM 45305GCA_003202285contigncbi588067

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
gram-positiveyes88.263yes
anaerobicno99.066no
halophileno54.572no
spore-formingyes80.232no
glucose-utilyes88.603no
thermophileno98.368yes
flagellatedno96.958no
aerobicyes89.534no
motileno90.972no
glucose-fermentno91.74no

External links

@ref: 16164

culture collection no.: DSM 45305, CCM 7635, CCUG 57426, NRRL B-24780, JCM 17974

straininfo link

  • @ref: 82522
  • straininfo: 408313

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny19651719Prauserella muralis sp. nov., from the indoor environment.Schafer J, Martin K, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.012005-02009Actinomycetales/*classification/genetics/physiology, Base Sequence, *Environmental Microbiology, Germany, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic AcidGenetics
Phylogeny26070693Prauserella soli sp. nov., isolated from crude oil-contaminated soil.Almutairi AInt J Syst Evol Microbiol10.1099/ijs.0.0003782015*Actinomycetales/classification, Base Composition, DNA, Bacterial/genetics, Environmental Pollution, Fatty Acids/chemistry, Molecular Sequence Data, Petroleum, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Soil MicrobiologyGenetics
Phylogeny31671051Prauserella flavalba sp. nov., a novel species of the genus Prauserella, isolated from alkaline soil.Li Q, Ruckert C, Li G, Huang P, Schneider O, Kalinowski J, Jiang Y, Zotchev SB, Jiang CInt J Syst Evol Microbiol10.1099/ijsem.0.0037682020Actinobacteria/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
16164Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45305)https://www.dsmz.de/collection/catalogue/details/culture/DSM-45305
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29384Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2579128776041
61322Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 57426)https://www.ccug.se/strain?id=57426
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
82522Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID408313.1StrainInfo: A central database for resolving microbial strain identifiers