Strain identifier

BacDive ID: 133188

Type strain: Yes

Species: Lacinutrix cladophorae

Strain Designation: 7Alg 4

Strain history: <- OI Nedashkovskaya, Pacific Institute of Bioorganic Chemistry, Russia

NCBI tax ID(s): 1778903 (species)

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General

@ref: 24984

BacDive-ID: 133188

keywords: 16S sequence, Bacteria, obligate aerobe, chemoheterotroph, Gram-negative, motile, rod-shaped, colony-forming, pigmented

description: Lacinutrix cladophorae 7Alg 4 is an obligate aerobe, chemoheterotroph, Gram-negative bacterium that forms circular colonies and has a yellow pigmentation.

NCBI tax id

  • NCBI tax id: 1778903
  • Matching level: species

strain history

  • @ref: 67771
  • history: <- OI Nedashkovskaya, Pacific Institute of Bioorganic Chemistry, Russia

doi: 10.13145/bacdive133188.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Flavobacteriia
  • order: Flavobacteriales
  • family: Flavobacteriaceae
  • genus: Lacinutrix
  • species: Lacinutrix cladophorae
  • full scientific name: Lacinutrix cladophorae Nedashkovskaya et al. 2016

@ref: 24984

domain: Bacteria

phylum: Bacteroidetes

class: Flavobacteriia

order: Flavobacteriales

family: Flavobacteriaceae

genus: Lacinutrix

species: Lacinutrix cladophorae

strain designation: 7Alg 4

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangement
24984negative1.2-2.3 µm0.4-0.5 µmrod-shapedyes
67771rod-shapedyesgliding
67771negative

colony morphology

  • @ref: 24984
  • colony size: 2.0-3.0 mm
  • colony color: yellow
  • colony shape: circular
  • medium used: MA (marine agar)

pigmentation

@refproductioncolorname
24984yesyellow
24984yesFlexirubin-type

Culture and growth conditions

culture medium

  • @ref: 24984
  • name: Marine agar (MA)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
24984positivegrowth4.0-32.0
24984positiveoptimum25.0-28.0mesophilic
67771positivegrowth25-28mesophilic

culture pH

@refabilitytypepHPH range
24984positiveoptimum7.5
24984positivegrowth5.5-9.5alkaliphile

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
24984obligate aerobe
67771aerobe

nutrition type

  • @ref: 24984
  • type: chemoheterotroph

spore formation

  • @ref: 24984
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
24984NaClpositivegrowth1.0-5.0 %
24984NaCloptimum2.5-4.0 %

observation

  • @ref: 67771
  • observation: quinones: MK-6

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
249842509agar-hydrolysis
24984casein-hydrolysis
2498417057cellobiose-builds acid from
2498417029chitin-hydrolysis
2498416947citrate-builds acid from
2498416947citrate-carbon source
2498415824D-fructose-builds acid from
2498412936D-galactose-builds acid from
2498417634D-glucose-builds acid from
2498416024D-mannose-builds acid from
2498465327D-xylose-builds acid from
2498416991dna-hydrolysis
2498424265gluconate-carbon source
2498417754glycerol-builds acid from
2498417268myo-inositol-builds acid from
2498430849L-arabinose-builds acid from
2498462345L-rhamnose-builds acid from
2498417716lactose-builds acid from
2498428053melibiose-builds acid from
24984506227N-acetylglucosamine-builds acid from
2498417632nitrate-reduction
2498416634raffinose-builds acid from
2498433942ribose-builds acid from
2498430911sorbitol-builds acid from
2498417992sucrose-builds acid from
2498427082trehalose-builds acid from
2498427897tryptophan-energy source
2498453424tween 20-hydrolysis
2498453423tween 40-hydrolysis
2498416199urea-hydrolysis
2498417634D-glucose+carbon source
2498416899D-mannitol+builds acid from
2498416024D-mannose+carbon source
249844853esculin+hydrolysis
249845291gelatin+hydrolysis
2498430849L-arabinose+carbon source
2498417716lactose+carbon source
2498417306maltose+builds acid from
2498417306maltose+carbon source
2498429864mannitol+carbon source
2498437684mannose+carbon source
2498430911sorbitol+carbon source
2498428017starch+hydrolysis
2498417992sucrose+carbon source
2498453426tween 80+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis sensitiveis resistant
2498428971ampicillinyesno
2498418208penicillin gyesno
24984474053cefazolinyesno
2498417833gentamicinyesno
249847507neomycinyesno
2498417076streptomycinyesno
2498427902tetracyclineyesno
249847809oxacillinyesno
249843393carbenicillinyesyes
2498450845doxycyclineyesyes
24984100147nalidixic acidyesyes
249843534cephalexinyesyes
2498428077rifampicinyesyes
2498417698chloramphenicolyesyes
2498448923erythromycinyesyes
249846472lincomycinyesyes
249847731ofloxacinyesyes
2498416869oleandomycinyesyes
2498428001vancomycinyesyes
249846104kanamycinyesyes
2498459062polymyxinyesyes

metabolite production

@refChebi-IDmetaboliteproduction
2498415688acetoinno
2498416136hydrogen sulfideno
2498435581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testcitrate test
2498435581indole-
2498416947citrate-

enzymes

@refvalueactivityec
24984acid phosphatase+3.1.3.2
24984alkaline phosphatase+3.1.3.1
24984alpha-chymotrypsin-3.4.21.1
24984alpha-fucosidase-3.2.1.51
24984alpha-galactosidase-3.2.1.22
24984alpha-glucosidase+3.2.1.20
24984alpha-mannosidase-3.2.1.24
24984arginine dihydrolase-3.5.3.6
24984beta-galactosidase-3.2.1.23
24984beta-glucosidase-3.2.1.21
24984beta-glucuronidase-3.2.1.31
24984catalase+1.11.1.6
24984cystine arylamidase+3.4.11.3
24984cytochrome oxidase+1.9.3.1
24984esterase (C 4)+
24984esterase Lipase (C 8)+
24984leucine arylamidase+3.4.11.1
24984lipase (C 14)-
24984lysine decarboxylase-4.1.1.18
24984N-acetyl-beta-glucosaminidase-3.2.1.52
24984naphthol-AS-BI-phosphohydrolase+
24984ornithine decarboxylase-4.1.1.17
24984trypsin-3.4.21.4
24984tryptophan deaminase-4.1.99.1
24984valine arylamidase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
24984green alga Cladophora stimpsoniiTroitsa Bay, Gulf of Peter the Great, Sea of Japan (also is known as East Sea), Pacific Ocean, RussiaRussiaRUSAsia42134
67771From the green alga `Cladophora stimpsonii`Troitsa Bay, Gulf of Peter the Great, East Sea(Sea of Japan), Pacific OceanRussiaRUSAsia

isolation source categories

  • Cat1: #Host
  • Cat2: #Algae
  • Cat3: #Green algae

Sequence information

16S sequences

  • @ref: 24984
  • description: Lacinutrix sp. 7Alg 4 16S ribosomal RNA gene, partial sequence
  • accession: KU510085
  • length: 1478
  • database: nuccore
  • NCBI tax ID: 1778903

GC content

@refGC-contentmethod
2498431.9thermal denaturation, midpoint method (Tm)
6777131.9

External links

@ref: 24984

culture collection no.: KCTC 23036, KMM 6381

literature

  • topic: Phylogeny
  • Pubmed-ID: 27473360
  • title: Lacinutrix cladophorae sp. nov., a flavobacterium isolated from the green alga Cladophora stimpsonii, transfer of Flavirhabdus iliipiscaria Shakeela et al. 2015 to the genus Lacinutrix as Lacinutrix iliipiscaria comb. nov. and emended description of the genus Lacinutrix.
  • authors: Nedashkovskaya OI, Kim SG, Zhukova NV, Lee JS, Mikhailov VV
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001353
  • year: 2016
  • mesh: Bacterial Typing Techniques, Base Composition, Chlorophyta/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Flavobacterium/*classification/genetics/isolation & purification, Japan, Phosphatidylethanolamines/chemistry, *Phylogeny, Pigmentation, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24984O. I. K. Nedashkovskaya, Song-Gun,Zhukova, Natalia V.,Lee, Jung-Sook,Mikhailov, Valery V.Lacinutrix cladophorae sp. nov., a flavobacterium isolated from the green alga Cladophora stimpsonii, transfer of Flavirhabdus iliipiscaria Shakeela et al. 2015 to the genus Lacinutrix as Lacinutrix iliipiscaria comb. nov. and emended description of the genus Lacinutrix10.1099/ijsem.0.001353IJSEM 66: 4339-4346 201627473360
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc