General

@ref: 24969

BacDive-ID: 133186

keywords: 16S sequence, Bacteria, mesophilic, Gram-positive, rod-shaped, colony-forming

description: Mycobacterium oryzae JC290 is a mesophilic, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from paddy soil.

NCBI tax id

• NCBI tax id: 1648871
• Matching level: species

doi: 10.13145/bacdive133186.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/actinomycetota
• domain: Bacteria
• phylum: Actinomycetota
• class: Actinomycetes
• order: Mycobacteriales
• family: Mycobacteriaceae
• genus: Mycobacterium
• species: Mycobacterium oryzae
• full scientific name: Mycobacterium oryzae Ramaprasad et al. 2016

• synonyms

  • @ref: 20215
  • synonym: Mycolicibacterium oryzae

@ref: 24969

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Mycobacteriales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium oryzae

strain designation: JC290

type strain: yes

Morphology

cell morphology

• @ref: 24969
• gram stain: positive
• cell length: 1.4-2.0 µm
• cell width: 0.4-0.6 µm
• cell shape: rod-shaped
• motility: no

colony morphology

• @ref: 24969
• colony size: 2.0-3.0 mm
• colony color: dark orange
• colony shape: circular
• incubation period: 3-5 days
• medium used: TSA (Trypticase soy agar)

pigmentation

• @ref: 24969
• production: yes
• color: beta-carotene
• name: Carotenoid

Culture and growth conditions

culture medium

culture temp

Physiology and metabolism

spore formation

• @ref: 24969
• spore formation: no

halophily

metabolite utilization

enzymes

Isolation, sampling and environmental information

isolation

• @ref: 24969
• sample type: paddy soil
• sampling date: 2014-11-01
• geographic location: Kankumbi,Western Ghats, Karnataka
• country: India
• origin.country: IND
• continent: Asia
• latitude: 15
• longitude: 74
• enrichment culture: R2A agar (Reasoner's 2A agar)
• enrichment culture composition: containing (g l-1) yeast extract (0.5), peptone (0.5), casein enzyme hydrolysate (0.5), yeast extract (0.5), glucose (0.5), water-soluble starch (0.5), dipotassium phosphate (0.3), magnesium sulphate (0.05) and sodium pyruvate (0.3);pH 7.0
• enrichment culture duration: 5 days
• enrichment culture temperature: 30

isolation source categories

Sequence information

16S sequences

• @ref: 24969
• description: Mycobacterium sp. JC290 partial 16S rRNA gene, strain JC290
• accession: LN846834
• length: 1419
• database: nuccore
• NCBI tax ID: 1648871

GC content

• @ref: 24969
• GC-content: 69.5-70.0
• method: high performance liquid chromatography (HPLC)

External links

@ref: 24969

culture collection no.: KCTC 39560, LMG 28809

literature

• topic: Phylogeny
• Pubmed-ID: 27499106
• title: Mycobacterium oryzae sp. nov., a scotochromogenic, rapidly growing species is able to infect human macrophage cell line.
• authors: Ramaprasad EVV, Rizvi A, Banerjee S, Sasikala C, Ramana CV
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijsem.0.001386
• year: 2016
• mesh: Bacterial Typing Techniques, Base Composition, Cell Line, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Humans, India, Macrophages/*microbiology, Mycobacterium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Oryza, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
• topic2: Pathogenicity

Reference