Strain identifier

BacDive ID: 133183

Type strain: Yes

Species: Microbacterium aureliae

Strain Designation: JF-6

Strain history: S. Mayilraj; MTCC, India; JF-6.

NCBI tax ID(s): 1452538 (species)

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General

@ref: 24972

BacDive-ID: 133183

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, rod-shaped, colony-forming

description: Microbacterium aureliae JF-6 is an aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from Aurelia aurita, the moon jellyfish.

NCBI tax id

  • NCBI tax id: 1452538
  • Matching level: species

strain history

  • @ref: 67770
  • history: S. Mayilraj; MTCC, India; JF-6.

doi: 10.13145/bacdive133183.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Microbacterium
  • species: Microbacterium aureliae
  • full scientific name: Microbacterium aureliae Kaur et al. 2016

@ref: 24972

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Micrococcales

family: Microbacteriaceae

genus: Microbacterium

species: Microbacterium aureliae

strain designation: JF-6

type strain: yes

Morphology

cell morphology

  • @ref: 24972
  • gram stain: positive
  • cell length: 0.8-1.0 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 24972
  • colony color: yellow
  • colony shape: circular
  • medium used: TSA (Trypticase soy agar)

Culture and growth conditions

culture medium

  • @ref: 24972
  • name: TSA (Trypticase soy agar)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
24972positivegrowth25.0-37.0mesophilic
24972positiveoptimum30mesophilic
24972nogrowth12psychrophilic
24972nogrowth42thermophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
24972positivegrowth8.0-11.0alkaliphile
24972positiveoptimum9
24972nogrowth5
24972positivegrowth8
24972positivegrowth11

Physiology and metabolism

oxygen tolerance

  • @ref: 24972
  • oxygen tolerance: aerobe

spore formation

  • @ref: 24972
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
24972NaClpositivegrowth0.0-10.0 %(w/v)
24972NaClpositivegrowth5 %(w/v)
24972NaClpositivegrowth7 %(w/v)
24972NaClpositivegrowth8 %(w/v)
24972NaClpositivegrowth9 %(w/v)
24972NaClpositivegrowth10 %(w/v)

murein

  • @ref: 24972
  • murein short key: B06
  • type: B2ß {Gly} [L-Hsr] D-Glu(Hyg)-Gly-D-Orn

observation

  • @ref: 67770
  • observation: quinones: MK-13, MK-12

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2497217019(R)-amygdalin-builds acid from
24972casein-hydrolysis
2497223456cyclodextrin-builds acid from
2497216988D-ribose-builds acid from
2497217924D-sorbitol-builds acid from
2497265327D-xylose-builds acid from
2497228260galactose-builds acid from
249725291gelatin-hydrolysis
2497217716lactose-builds acid from
24972320055methyl beta-D-glucopyranoside-builds acid from
24972506227N-acetylglucosamine-builds acid from
2497217632nitrate-reduction
2497227941pullulan-builds acid from
2497226546rhamnose-builds acid from
2497217992sucrose-builds acid from
2497217057cellobiose+builds acid from
2497217634D-glucose+builds acid from
2497228757fructose+builds acid from
2497217234glucose+fermentation
2497224996lactate+builds acid from
2497216634raffinose+builds acid from
2497228017starch+hydrolysis
2497227082trehalose+builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis resistant
249728309polymyxin byesno
2497228669bacitracinyesno
2497286455optochinyesno
2497228368novobiocinyesno

metabolite production

@refChebi-IDmetaboliteproduction
2497215688acetoinno
2497235581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-test
2497217234glucose+
2497215688acetoin-

enzymes

@refvalueactivityec
24972alanine arylamidase+3.4.11.2
24972alpha-galactosidase+3.2.1.22
24972alpha-glucosidase+3.2.1.20
24972alpha-mannosidase-3.2.1.24
24972arginine dihydrolase+3.5.3.6
24972beta-galactosidase-3.2.1.23
24972beta-glucuronidase-3.2.1.31
24972catalase+1.11.1.6
24972cytochrome oxidase+1.9.3.1
24972L-aspartate arylamidase-3.4.11.21
24972leucine arylamidase+3.4.11.1
24972phosphatase-
24972proline-arylamidase-3.4.11.5
24972pyrrolidonyl arylamidase-3.4.19.3
24972tripeptide aminopeptidase-3.4.11.4
24972tyrosine arylamidase+
24972urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureisolation procedurehost species
24972Aurelia aurita, the moon jellyfishBay of Bengal coast, KanyakumariIndiaINDAsia1387Zobell marine agar and tryptic soy agardilution-plate technique
67770Aurelia aurita, the moon jellyfishBay of Bengal coast, KanyakumariIndiaINDAsiaAurelia aurita

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Host#Invertebrates (Other)#Cnidaria (Corals)
#Climate#Temperate

Sequence information

16S sequences

  • @ref: 24972
  • description: Microbacterium sp. JF-6 16S ribosomal RNA gene, partial sequence
  • accession: KF793922
  • length: 1470
  • database: nuccore
  • NCBI tax ID: 1452538

GC content

@refGC-contentmethod
2497269.4Thermal denaturation, fluorometry
6777069.4thermal denaturation, midpoint method (Tm)

External links

@ref: 24972

culture collection no.: MTCC 11843, JCM 30060, KCTC 39828

literature

  • topic: Phylogeny
  • Pubmed-ID: 27506590
  • title: Microbacterium aureliae sp. nov., a novel actinobacterium isolated from Aurelia aurita, the moon jellyfish.
  • authors: Kaur G, Mual P, Kumar N, Verma A, Kumar A, Krishnamurthi S, Mayilraj S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001407
  • year: 2016
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, India, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Scyphozoa/*microbiology, Sequence Analysis, DNA, Vitamin K 2/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urljournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24972G. M. Kaur, Parveen,Kumar, Narender,Verma, Ashish,Kumar, Anand,Krishnamurthi, Srinivasan,Mayilraj, ShanmugamMicrobacterium aureliae sp. nov., a novel actinobacterium isolated from Aurelia aurita, the moon jellyfish10.1099/ijsem.0.001407IJSEM 66: 4665-4670 201627506590
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/