Strain identifier

BacDive ID: 133173

Type strain: Yes

Species: Massilia psychrophila

Culture col. no.: B1555-1 , CGMCC 1.15196, JCM 30813

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General

@ref: 24949

BacDive-ID: 133173

keywords: colony-forming, rod-shaped, motile, gram-negative, psychrophilic, spore-forming, aerobe, Bacteria, 16S sequence, genome sequence

description: Massilia psychrophila B1555-1 is an aerobe, spore-forming, psychrophilic bacterium that forms circular colonies and was isolated from ice core which was drilled from Ulugh Muztagh Glacier.

doi: 10.13145/bacdive133173.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/proteobacteria
  • domain: Bacteria
  • phylum: Proteobacteria
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Oxalobacteraceae
  • genus: Massilia
  • species: Massilia psychrophila
  • full scientific name: Massilia psychrophila Guo et al. 2016

@ref: 24949

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Oxalobacteraceae

genus: Massilia

species: Massilia psychrophila

type strain: yes

Morphology

cell morphology

  • @ref: 24949
  • gram stain: negative
  • cell length: 1.2-1.3 µm
  • cell width: 0.7-0.8 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 24949
  • colony color: yellow
  • colony shape: circular
  • incubation period: 4-5 days
  • medium used: R2A agar (Reasoner's 2A agar)

Culture and growth conditions

culture medium

  • @ref: 24949
  • name: Reasoner's 2A agar (R2A)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
24949positiveoptimum15psychrophilic
24949positivegrowth10.0-25.0

culture pH

@refabilitytypepH
24949positiveoptimum7
24949positivegrowth6.0-9.0

Physiology and metabolism

oxygen tolerance

  • @ref: 24949
  • oxygen tolerance: aerobe

spore formation

  • @ref: 24949
  • spore formation: yes

halophily

@refsalttested relationconcentrationgrowth
24949NaCloptimum1 %
24949NaClgrowth0.0-2.0 %(w/v)positive

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2494917128adipate-assimilation
2494922599arabinose-assimilation
2494916947citrate-assimilation
2494927689decanoate-assimilation
2494924265gluconate-assimilation
2494917234glucose-assimilation
2494925115malate-assimilation
2494917306maltose-assimilation
2494929864mannitol-assimilation
2494937684mannose-assimilation
24949506227N-acetylglucosamine-assimilation
2494917632nitrate-reduction
2494917309pectin-hydrolysis
2494918401phenylacetate-assimilation
2494928017starch-hydrolysis
2494953426tween 80-hydrolysis
249494853esculin+hydrolysis
249495291gelatin+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
2494916136hydrogen sulfideno
2494935581indoleno

enzymes

@refvalueactivityec
24949acid phosphatase+3.1.3.2
24949alkaline phosphatase+3.1.3.1
24949alpha-fucosidase-3.2.1.51
24949alpha-galactosidase-3.2.1.22
24949alpha-glucosidase+3.2.1.20
24949alpha-mannosidase-3.2.1.24
24949beta-galactosidase-3.2.1.23
24949beta-glucosidase-3.2.1.21
24949catalase+1.11.1.6
24949chymotrypsin+3.4.4.5
24949cystine arylamidase-3.4.11.3
24949cytochrome oxidase+1.9.3.1
24949esterase (C 4)+
24949esterase lipase (C 8)+
24949leucine arylamidase+3.4.11.1
24949lipase (C 14)-
24949N-acetyl-beta-glucosaminidase-3.2.1.52
24949naphthol-AS-BI-phosphohydrolase+
24949trypsin-3.4.21.4
24949urease-3.5.1.5
24949valine arylamidase+

Isolation, sampling and environmental information

isolation

  • @ref: 24949
  • sample type: ice core which was drilled from Ulugh Muztagh Glacier
  • geographic location: ice cap Ulugh Muztagh (highest part of the central Kunlun Mountains)
  • country: Republic of China
  • origin.country: CHN
  • continent: Asia
  • latitude: 35
  • longitude: 87
  • enrichment culture: R2A agar (Reasoner's 2A agar)
  • enrichment culture duration: 30 days
  • enrichment culture temperature: 4

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Glacier
#Environmental#Aquatic#Ice
#Environmental#Terrestrial#Core sample

Sequence information

16S sequences

  • @ref: 24949
  • description: Massilia sp. B1555-1 16S ribosomal RNA gene, partial sequence
  • accession: KM873051
  • length: 1415
  • database: nuccore
  • NCBI tax ID: 1603353

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Massilia psychrophila JCM 30813GCA_002760665contigncbi1603353
66792Massilia psychrophila strain JCM 308131603353.3wgspatric1603353

GC content

  • @ref: 24949
  • GC-content: 66

External links

@ref: 24949

culture collection no.: B1555-1, CGMCC 1.15196, JCM 30813

Reference

@idauthorstitledoi/urljournal
20215D.Gleim, M.Kracht, N.Weiss et. al.Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes sincehttp://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html
24949B. L. Guo, Yongqin,Gu, Zhengquan,Shen, Liang,Liu, Keshao,Wang, Ningliang,Xing, Tingting,Liu, Hongcan,Zhou, Yuguang,Li, JiuleMassilia psychrophila sp. nov., isolated from an ice core10.1099/ijsem.0.001315IJSEM 66: 4088-4093 2016
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes