Strain identifier

BacDive ID: 13317

Type strain: Yes

Species: Prauserella alba

Strain history: CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 90005 <- DSMZ <- CCTCC <- CCRC

NCBI tax ID(s): 176898 (species)

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General

@ref: 11905

BacDive-ID: 13317

DSM-Number: 44590

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, rod-shaped

description: Prauserella alba DSM 44590 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from soil in hypersaline habitats.

NCBI tax id

  • NCBI tax id: 176898
  • Matching level: species

strain history

@refhistory
11905<- C.-L. Jiang, YIM (Prauserella dehalogenans)
67770KCTC 19002 <-- W.-J. Li YIM 90005.
116179CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 90005 <- DSMZ <- CCTCC <- CCRC

doi: 10.13145/bacdive13317.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Prauserella
  • species: Prauserella alba
  • full scientific name: Prauserella alba Li et al. 2003

@ref: 11905

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Prauserella

species: Prauserella alba

full scientific name: Prauserella alba Li et al. 2003 emend. Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

  • @ref: 116179
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colormedium used
69357Ivory (1014)suter with tyrosine
69357Ivory (1014)suter without tyrosine
69357Light ivory (1015)ISP 2
69357Light ivory (1015)ISP 4
69357Light ivory (1015)ISP 6
69357Light ivory (1015)ISP 7
69357Oyster white (10113)ISP 5
69357Oyster white (1013)ISP 3

multicellular morphology

@refforms multicellular complexcomplex namemedium name
69357noAerial myceliumISP 2
69357noAerial myceliumISP 3
69357noAerial myceliumISP 4
69357noAerial myceliumISP 5
69357noAerial myceliumISP 6
69357noAerial myceliumISP 7
69357noAerial myceliumsuter with tyrosine
69357noAerial myceliumsuter without tyrosine

pigmentation

@refproductionnamecolor
69357noMelanin
69357yessoluble pigmentBeige (1001)

multimedia

@refmultimedia contentcaptionintellectual property rights
69357DSM_44590_image3.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH
69357DSM_44590_image4.jpegPlates (65, ISP2, ISP3, ISP4, ISP5, ISP7)Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11905BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
11905GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65; with strain-specific modifications) Composition: NaCl 100.0 g/l Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
33866Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
116179CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
11905positivegrowth28mesophilic
19678positiveoptimum28mesophilic
33866positivegrowth30mesophilic
67770positivegrowth28mesophilic
116179positivegrowth22-41
116179nogrowth5psychrophilic
116179nogrowth15psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116179
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
69357NaClpositivegrowth0-7.5 %
116179NaClpositivegrowth4-10 %
116179NaClnogrowth0 %
116179NaClnogrowth2 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
69357arabinose+growth22599
69357cellulose+/-growth62968
69357fructose+/-growth28757
69357glucose+growth17234
69357inositol+growth17268
69357mannose+growth37684
69357raffinose-growth16634
69357rhamnose+/-growth26546
69357sucrose+growth17992
69357xylose+growth18222
116179citrate-carbon source16947
116179esculin-hydrolysis4853
116179glucose-fermentation17234
116179lactose-fermentation17716
116179nitrate-reduction17632
116179nitrite-reduction16301
116179sodium thiosulfate-builds gas from132112
68379nitrate-reduction17632
68379esculin-hydrolysis4853
68379urea-hydrolysis16199
68379gelatin+hydrolysis5291
68379D-glucose-fermentation17634
68379D-ribose-fermentation16988
68379D-xylose-fermentation65327
68379D-mannitol-fermentation16899
68379maltose-fermentation17306
68379lactose-fermentation17716
68379sucrose-fermentation17992
68379glycogen-fermentation28087

metabolite production

  • @ref: 116179
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 116179
  • Chebi-ID: 17234
  • metabolite: glucose
  • methylred-test: -

enzymes

@refvalueactivityec
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
116179oxidase-
116179beta-galactosidase+3.2.1.23
116179alcohol dehydrogenase-1.1.1.1
116179gelatinase+/-
116179amylase+
116179caseinase+3.4.21.50
116179catalase+1.11.1.6
116179tween esterase+
116179gamma-glutamyltransferase-2.3.2.2
116179lecithinase+
116179lipase+
116179lysine decarboxylase-4.1.1.18
116179ornithine decarboxylase-4.1.1.17
116179phenylalanine ammonia-lyase-4.3.1.24
116179tryptophan deaminase-
116179urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382alpha-galactosidase-3.2.1.22
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
69357---+--+---+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
69357++/-++/-++/-+/---+/-+/----+----
116179+++-+----++-+-+-----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116179-------------------------------------------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116179--++---++++----------+-+--+------+---+--+--+-----+-+---+----+---+-+------------++------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationisolation date
11905soil in hypersaline habitatsChinaCHNAsia
67770Soil from hypersaline habitatsChinaCHNAsiaXinjang Province
116179Environment, Soil, hypersalineChinaCHNAsiaXinjiang2000

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_41937.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_3364;97_4129;98_30482;99_41937&stattab=map
  • Last taxonomy: Prauserella
  • 16S sequence: AF435077
  • Sequence Identity:
  • Total samples: 322
  • soil counts: 41
  • aquatic counts: 15
  • animal counts: 255
  • plant counts: 11

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
119051Risk group (German classification)
196781Risk group (German classification)
1161791Risk group (French classification)

Sequence information

16S sequences

  • @ref: 11905
  • description: Prauserella alba strain YIM 90005 16S ribosomal RNA gene, partial sequence
  • accession: AF435077
  • length: 1503
  • database: ena
  • NCBI tax ID: 176898

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prauserella alba DSM 445902596583529draftimg176898
66792Prauserella alba DSM 44590GCA_024171735scaffoldncbi176898

GC content

@refGC-contentmethod
1190566.7
6777066.7thermal denaturation, midpoint method (Tm)
6777070.1genome sequence analysis

External links

@ref: 11905

culture collection no.: DSM 44590, CCTCC AA 001016, YIM 90005, JCM 13022, BCRC 16313, CIP 107981, KCTC 19002, CCRC 16313

straininfo link

  • @ref: 82518
  • straininfo: 113681

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130045Prauserella halophila sp. nov. and Prauserella alba sp. nov., moderately halophilic actinomycetes from saline soil.Li WJ, Xu P, Tang SK, Xu LH, Kroppenstedt RM, Stackebrandt E, Jiang CLInt J Syst Evol Microbiol10.1099/ijs.0.02611-02003Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Base Composition, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Microscopy, Electron, Scanning, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium Chloride, Soil MicrobiologyGenetics
Phylogeny19651719Prauserella muralis sp. nov., from the indoor environment.Schafer J, Martin K, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.012005-02009Actinomycetales/*classification/genetics/physiology, Base Sequence, *Environmental Microbiology, Germany, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic AcidGenetics
Metabolism21800621[Cloning and characterization of gene cluster for biosynthesis of ectoine and 5-hydroxyectoine from extreme halotolerant actinomycete strain Prauserella alba YIM 90005(T)].Li Y, Dong L, Wang L, Fang F, He M, Cao Z, Liang Y, Tang S, Li WWei Sheng Wu Xue Bao2011Actinomycetales/drug effects/*genetics/metabolism, Acyltransferases/genetics/metabolism, Amino Acid Sequence, Amino Acids, Diamino/analysis/*biosynthesis/*genetics, Bacterial Proteins/genetics/metabolism, Base Sequence, Cloning, Molecular, Gene Expression Regulation, Bacterial, Genes, Bacterial/genetics, Hydro-Lyases/genetics/metabolism, Molecular Sequence Data, Multigene Family/*genetics, Osmotic Pressure, Sequence Analysis, DNA, Sodium Chloride/pharmacology, Transaminases/genetics/metabolismEnzymology
Phylogeny25713039Prauserella isguenensis sp. nov., a halophilic actinomycete isolated from desert soil.Saker R, Bouras N, Meklat A, Zitouni A, Schumann P, Sproer C, Sabaou N, Klenk HPInt J Syst Evol Microbiol10.1099/ijs.0.0001452015Actinomycetales/*classification/genetics/isolation & purification, Algeria, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, *Desert Climate, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Molecular Sequence Data, Nucleic Acid Hybridization, Phosphatidylethanolamines/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistryGenetics

Reference

@idauthorscataloguedoi/urltitle
11905Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44590)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44590
19678Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44590.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33866Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5552
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69357Wink, J.https://cdn.dsmz.de/wink/DSM%2044590.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
82518Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID113681.1StrainInfo: A central database for resolving microbial strain identifiers
116179Curators of the CIPCollection of Institut Pasteur (CIP 107981)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107981