Strain identifier

BacDive ID: 13316

Type strain: Yes

Species: Prauserella halophila

Strain history: CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 90001 <- DSMZ <- CCTCC <- CCRC

NCBI tax ID(s): 185641 (species)

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General

@ref: 11930

BacDive-ID: 13316

DSM-Number: 44617

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-positive, rod-shaped

description: Prauserella halophila DSM 44617 is a mesophilic, Gram-positive, rod-shaped bacterium that was isolated from soil in hypersaline habitat.

NCBI tax id

  • NCBI tax id: 185641
  • Matching level: species

strain history

@refhistory
11930<- W.-J. Li, YIM
67770KCTC 19000 <-- W.-J. Li YIM 90001.
119809CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 90001 <- DSMZ <- CCTCC <- CCRC

doi: 10.13145/bacdive13316.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Prauserella
  • species: Prauserella halophila
  • full scientific name: Prauserella halophila Li et al. 2003

@ref: 11930

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Prauserella

species: Prauserella halophila

full scientific name: Prauserella halophila Li et al. 2003 emend. Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

  • @ref: 119809
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
19683Beige10-14 daysISP 2
19683Beige10-14 daysISP 3
19683Beige10-14 daysISP 4
19683Beige10-14 daysISP 5
19683Beige10-14 daysISP 6
19683Beige10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19683noISP 2
19683noISP 3
19683noISP 4
19683noISP 5
19683noISP 6
19683noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11930GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65; with strain-specific modifications) Composition: NaCl 100.0 g/l Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19683ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19683ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19683ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19683ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19683ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19683ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
33186Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
119809CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
11930positivegrowth28mesophilic
19683positiveoptimum28mesophilic
33186positivegrowth30mesophilic
67770positivegrowth28mesophilic
119809positivegrowth25-37mesophilic
119809nogrowth10psychrophilic
119809nogrowth15psychrophilic
119809nogrowth41thermophilic
119809nogrowth45thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
119809NaClpositivegrowth6-10 %
119809NaClnogrowth0 %
119809NaClnogrowth2 %
119809NaClnogrowth4 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1968317234glucose-
1968322599arabinose-
1968317992sucrose+
1968318222xylose-
1968317268myo-inositol-
1968329864mannitol+
1968328757fructose+
1968326546rhamnose-
1968316634raffinose-
1968362968cellulose-
11980916947citrate-carbon source
1198094853esculin-hydrolysis
11980917632nitrate-reduction
11980916301nitrite-reduction

metabolite production

  • @ref: 119809
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11980915688acetoin+
11980917234glucose-

enzymes

@refvalueactivityec
119809oxidase-
119809beta-galactosidase-3.2.1.23
119809alcohol dehydrogenase-1.1.1.1
119809gelatinase-
119809catalase+1.11.1.6
119809gamma-glutamyltransferase-2.3.2.2
119809lysine decarboxylase-4.1.1.18
119809ornithine decarboxylase-4.1.1.17
119809phenylalanine ammonia-lyase-4.3.1.24
119809tryptophan deaminase-
119809urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refLipaseCystine arylamidaseTrypsinalpha- ChymotrypsinNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- GlucuronidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControlAlkaline phosphataseEsteraseEsterase LipaseLeucine arylamidaseValine arylamidaseAcid phosphatasealpha- Glucosidasebeta- Glucosidase
19683-----------
119809----+-------++++----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
11930soil in hypersaline habitatLingiang provinceChinaCHNAsia
67770Soil from hypersaline habitatsXinjang ProvinceChinaCHNAsia
119809Environment, Soil, hypersalineXinjiangChinaCHNAsia1998

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Saline

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
119301Risk group (German classification)
196831Risk group (German classification)
1198091Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Prauserella halophila strain YIM 90001 16S ribosomal RNA gene, partial sequence
  • accession: AF466190
  • length: 1514
  • database: ena
  • NCBI tax ID: 185641

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Prauserella halophila DSM 446172596583595draftimg185641
66792Prauserella halophila DSM 44617GCA_024171895scaffoldncbi185641

GC content

@refGC-contentmethod
6777069.8genome sequence analysis
6777065.8thermal denaturation, midpoint method (Tm)

External links

@ref: 11930

culture collection no.: DSM 44617, YIM 90001, JCM 13023, BCRC 16311, CCTCC AA 001015, CIP 107978, KCTC 19000

straininfo link

  • @ref: 82517
  • straininfo: 128007

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny13130045Prauserella halophila sp. nov. and Prauserella alba sp. nov., moderately halophilic actinomycetes from saline soil.Li WJ, Xu P, Tang SK, Xu LH, Kroppenstedt RM, Stackebrandt E, Jiang CLInt J Syst Evol Microbiol10.1099/ijs.0.02611-02003Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Base Composition, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Microscopy, Electron, Scanning, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium Chloride, Soil MicrobiologyGenetics
Phylogeny19651719Prauserella muralis sp. nov., from the indoor environment.Schafer J, Martin K, Kampfer PInt J Syst Evol Microbiol10.1099/ijs.0.012005-02009Actinomycetales/*classification/genetics/physiology, Base Sequence, *Environmental Microbiology, Germany, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic AcidGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11930Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44617)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44617
19683Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44617.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33186Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5548
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
82517Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID128007.1StrainInfo: A central database for resolving microbial strain identifiers
119809Curators of the CIPCollection of Institut Pasteur (CIP 107978)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107978