Strain identifier
BacDive ID: 13316
Type strain:
Species: Prauserella halophila
Strain history: CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 90001 <- DSMZ <- CCTCC <- CCRC
NCBI tax ID(s): 185641 (species)
General
@ref: 11930
BacDive-ID: 13316
DSM-Number: 44617
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-positive, rod-shaped
description: Prauserella halophila DSM 44617 is a mesophilic, Gram-positive, rod-shaped bacterium that was isolated from soil in hypersaline habitat.
NCBI tax id
- NCBI tax id: 185641
- Matching level: species
strain history
@ref | history |
---|---|
11930 | <- W.-J. Li, YIM |
67770 | KCTC 19000 <-- W.-J. Li YIM 90001. |
119809 | CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM 90001 <- DSMZ <- CCTCC <- CCRC |
doi: 10.13145/bacdive13316.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Pseudonocardiales
- family: Pseudonocardiaceae
- genus: Prauserella
- species: Prauserella halophila
- full scientific name: Prauserella halophila Li et al. 2003
@ref: 11930
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Pseudonocardiaceae
genus: Prauserella
species: Prauserella halophila
full scientific name: Prauserella halophila Li et al. 2003 emend. Nouioui et al. 2018
type strain: yes
Morphology
cell morphology
- @ref: 119809
- gram stain: positive
- cell shape: rod-shaped
- motility: no
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19683 | Beige | 10-14 days | ISP 2 |
19683 | Beige | 10-14 days | ISP 3 |
19683 | Beige | 10-14 days | ISP 4 |
19683 | Beige | 10-14 days | ISP 5 |
19683 | Beige | 10-14 days | ISP 6 |
19683 | Beige | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
19683 | no | ISP 2 |
19683 | no | ISP 3 |
19683 | no | ISP 4 |
19683 | no | ISP 5 |
19683 | no | ISP 6 |
19683 | no | ISP 7 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11930 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://mediadive.dsmz.de/medium/65 | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65; with strain-specific modifications) Composition: NaCl 100.0 g/l Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water |
19683 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19683 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19683 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19683 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19683 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19683 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
33186 | Marine agar (MA) | yes | Distilled water make up to (1000.000 ml);Marine agar (55.100 g) | |
119809 | CIP Medium 13 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
11930 | positive | growth | 28 | mesophilic |
19683 | positive | optimum | 28 | mesophilic |
33186 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
119809 | positive | growth | 25-37 | mesophilic |
119809 | no | growth | 10 | psychrophilic |
119809 | no | growth | 15 | psychrophilic |
119809 | no | growth | 41 | thermophilic |
119809 | no | growth | 45 | thermophilic |
Physiology and metabolism
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
119809 | NaCl | positive | growth | 6-10 % |
119809 | NaCl | no | growth | 0 % |
119809 | NaCl | no | growth | 2 % |
119809 | NaCl | no | growth | 4 % |
observation
- @ref: 67770
- observation: quinones: MK-9(H4)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
19683 | 17234 | glucose | - | |
19683 | 22599 | arabinose | - | |
19683 | 17992 | sucrose | + | |
19683 | 18222 | xylose | - | |
19683 | 17268 | myo-inositol | - | |
19683 | 29864 | mannitol | + | |
19683 | 28757 | fructose | + | |
19683 | 26546 | rhamnose | - | |
19683 | 16634 | raffinose | - | |
19683 | 62968 | cellulose | - | |
119809 | 16947 | citrate | - | carbon source |
119809 | 4853 | esculin | - | hydrolysis |
119809 | 17632 | nitrate | - | reduction |
119809 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 119809
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
119809 | 15688 | acetoin | + | |
119809 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
119809 | oxidase | - | |
119809 | beta-galactosidase | - | 3.2.1.23 |
119809 | alcohol dehydrogenase | - | 1.1.1.1 |
119809 | gelatinase | - | |
119809 | catalase | + | 1.11.1.6 |
119809 | gamma-glutamyltransferase | - | 2.3.2.2 |
119809 | lysine decarboxylase | - | 4.1.1.18 |
119809 | ornithine decarboxylase | - | 4.1.1.17 |
119809 | phenylalanine ammonia-lyase | - | 4.3.1.24 |
119809 | tryptophan deaminase | - | |
119809 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Lipase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Leucine arylamidase | Valine arylamidase | Acid phosphatase | alpha- Glucosidase | beta- Glucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19683 | - | - | - | - | - | - | - | - | - | - | - | |||||||||
119809 | - | - | - | - | + | - | - | - | - | - | - | - | + | + | + | + | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
11930 | soil in hypersaline habitat | Lingiang province | China | CHN | Asia | |
67770 | Soil from hypersaline habitats | Xinjang Province | China | CHN | Asia | |
119809 | Environment, Soil, hypersaline | Xinjiang | China | CHN | Asia | 1998 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Soil |
#Condition | #Saline |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
11930 | 1 | Risk group (German classification) |
19683 | 1 | Risk group (German classification) |
119809 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 20218
- description: Prauserella halophila strain YIM 90001 16S ribosomal RNA gene, partial sequence
- accession: AF466190
- length: 1514
- database: ena
- NCBI tax ID: 185641
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Prauserella halophila DSM 44617 | 2596583595 | draft | img | 185641 |
66792 | Prauserella halophila DSM 44617 | GCA_024171895 | scaffold | ncbi | 185641 |
GC content
@ref | GC-content | method |
---|---|---|
67770 | 69.8 | genome sequence analysis |
67770 | 65.8 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 11930
culture collection no.: DSM 44617, YIM 90001, JCM 13023, BCRC 16311, CCTCC AA 001015, CIP 107978, KCTC 19000
straininfo link
- @ref: 82517
- straininfo: 128007
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 13130045 | Prauserella halophila sp. nov. and Prauserella alba sp. nov., moderately halophilic actinomycetes from saline soil. | Li WJ, Xu P, Tang SK, Xu LH, Kroppenstedt RM, Stackebrandt E, Jiang CL | Int J Syst Evol Microbiol | 10.1099/ijs.0.02611-0 | 2003 | Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Base Composition, China, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/genetics, Microscopy, Electron, Scanning, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sodium Chloride, Soil Microbiology | Genetics |
Phylogeny | 19651719 | Prauserella muralis sp. nov., from the indoor environment. | Schafer J, Martin K, Kampfer P | Int J Syst Evol Microbiol | 10.1099/ijs.0.012005-0 | 2009 | Actinomycetales/*classification/genetics/physiology, Base Sequence, *Environmental Microbiology, Germany, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid | Genetics |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11930 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44617) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44617 | |||
19683 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44617.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
33186 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/5548 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68382 | Automatically annotated from API zym | |||||
82517 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID128007.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
119809 | Curators of the CIP | Collection of Institut Pasteur (CIP 107978) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107978 |