Strain identifier
BacDive ID: 133106
Type strain:
Species: Actinotalea caeni
Strain Designation: EBR-4-2
Strain history: L. Jin; KRIBB, South Korea; EBR-4-2.
NCBI tax ID(s): 1348467 (species)
General
@ref: 25310
BacDive-ID: 133106
keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-positive, rod-shaped, colony-forming
description: Actinotalea caeni EBR-4-2 is an aerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from sludge of a biofilm reactor.
NCBI tax id
- NCBI tax id: 1348467
- Matching level: species
strain history
- @ref: 67770
- history: L. Jin; KRIBB, South Korea; EBR-4-2.
doi: 10.13145/bacdive133106.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Micrococcales
- family: Cellulomonadaceae
- genus: Actinotalea
- species: Actinotalea caeni
- full scientific name: Actinotalea caeni Jin et al. 2017
synonyms
- @ref: 20215
- synonym: Pseudactinotalea caeni
@ref: 25310
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Micrococcales
family: Cellulomonadaceae
genus: Actinotalea
species: Actinotalea caeni
strain designation: EBR-4-2
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell shape | motility | confidence |
---|---|---|---|---|
25310 | positive | rod-shaped | no | |
69480 | positive | 100 |
colony morphology
- @ref: 25310
- colony color: light yellow
- colony shape: circular
- medium used: R2A
Culture and growth conditions
culture medium
- @ref: 25310
- name: Reasoner's 2A agar (R2A)
- growth: yes
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
25310 | positive | growth | 10-55 | |
25310 | positive | optimum | 30 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
25310 | positive | growth | 6.0-9.0 | alkaliphile |
25310 | positive | optimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 25310
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
25310 | no | |
69481 | yes | 90 |
69480 | no | 99.983 |
halophily
@ref | salt | tested relation | concentration | growth |
---|---|---|---|---|
25310 | NaCl | optimum | 3.0-5.0 % | |
25310 | NaCl | growth | 1.0-8.0 %(w/v) | positive |
observation
- @ref: 67770
- observation: quinones: MK-10(H4)
metabolite utilization
@ref | metabolite | utilization activity | kind of utilization tested | Chebi-ID |
---|---|---|---|---|
25310 | 2-oxogluconate | - | assimilation | |
25310 | 3-hydroxybenzoate | - | assimilation | 16193 |
25310 | 3-hydroxybutyrate | - | assimilation | 37054 |
25310 | 4-hydroxybenzoate | - | assimilation | 17879 |
25310 | 5-dehydro-D-gluconate | - | assimilation | 58143 |
25310 | adipate | - | assimilation | 17128 |
25310 | butyrate | - | assimilation | 17968 |
25310 | citrate | - | assimilation | 16947 |
25310 | D-mannose | - | assimilation | 16024 |
25310 | D-ribose | - | assimilation | 16988 |
25310 | D-sorbitol | - | assimilation | 17924 |
25310 | gluconate | - | assimilation | 24265 |
25310 | glucose | - | builds acid from | 17234 |
25310 | histidine | - | assimilation | 27570 |
25310 | myo-inositol | - | assimilation | 17268 |
25310 | itaconate | - | assimilation | 17240 |
25310 | L-alanine | - | assimilation | 16977 |
25310 | L-fucose | - | assimilation | 18287 |
25310 | L-proline | - | assimilation | 17203 |
25310 | L-rhamnose | - | assimilation | 62345 |
25310 | L-serine | - | assimilation | 17115 |
25310 | malate | - | assimilation | 25115 |
25310 | nitrate | - | reduction | 17632 |
25310 | phenylacetate | - | assimilation | 18401 |
25310 | propionate | - | assimilation | 17272 |
25310 | salicin | - | assimilation | 17814 |
25310 | suberic acid | - | assimilation | 9300 |
25310 | valerate | - | assimilation | 31011 |
25310 | acetate | + | assimilation | 30089 |
25310 | D-glucose | + | assimilation | 17634 |
25310 | D-mannitol | + | assimilation | 16899 |
25310 | esculin | + | hydrolysis | 4853 |
25310 | gelatin | + | hydrolysis | 5291 |
25310 | glycogen | + | assimilation | 28087 |
25310 | L-arabinose | + | assimilation | 30849 |
25310 | lactate | + | assimilation | 24996 |
25310 | maltose | + | assimilation | 17306 |
25310 | melibiose | + | assimilation | 28053 |
25310 | N-acetylglucosamine | + | assimilation | 506227 |
25310 | sucrose | + | assimilation | 17992 |
metabolite production
- @ref: 25310
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
25310 | acid phosphatase | + | 3.1.3.2 |
25310 | alkaline phosphatase | - | 3.1.3.1 |
25310 | alpha-chymotrypsin | + | 3.4.21.1 |
25310 | alpha-fucosidase | - | 3.2.1.51 |
25310 | alpha-galactosidase | - | 3.2.1.22 |
25310 | alpha-glucosidase | + | 3.2.1.20 |
25310 | alpha-mannosidase | + | 3.2.1.24 |
25310 | arginine dihydrolase | - | 3.5.3.6 |
25310 | beta-galactosidase | + | 3.2.1.23 |
25310 | beta-glucosidase | - | 3.2.1.21 |
25310 | beta-glucuronidase | - | 3.2.1.31 |
25310 | catalase | + | 1.11.1.6 |
25310 | cystine arylamidase | + | 3.4.11.3 |
25310 | cytochrome oxidase | + | 1.9.3.1 |
25310 | esterase (C 4) | + | |
25310 | esterase Lipase (C 8) | + | |
25310 | leucine arylamidase | + | 3.4.11.1 |
25310 | lipase (C 14) | + | |
25310 | N-acetyl-beta-glucosaminidase | + | 3.2.1.52 |
25310 | naphthol-AS-BI-phosphohydrolase | + | |
25310 | trypsin | + | 3.4.21.4 |
25310 | urease | - | 3.5.1.5 |
25310 | valine arylamidase | + |
fatty acid profile
fatty acids
@ref fatty acid percentage 25310 C15:0 anteiso 32.8 25310 C15:1 anteiso A 9.4 25310 C17:0 anteiso 4.2 25310 C14:0 3.1 25310 C16:0 23.1 25310 C17:0 1.8 25310 C18:0 0.7 25310 C14:0 iso 1.5 25310 C15:0 iso 5.3 25310 C16:0 iso 2 25310 C17:0 iso 1.6 25310 N alcohol C16:0 14.6 - type of FA analysis: whole cell analysis
- incubation medium: Tryptic soy agar (TSA)
- agar/liquid: agar
- incubation temperature: 30
- incubation time: 2
- system: MIS MIDI
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
25310 | sludge of a biofilm reactor | Daejeon, Republic of Korea | Republic of Korea | KOR | Asia |
67770 | Biofilm reactor | Daejeon | Republic of Korea | KOR | Asia |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Bioreactor | |
#Environmental | #Terrestrial | #Mud (Sludge) |
#Environmental | #Biofilm |
taxonmaps
- @ref: 69479
- File name: preview.99_59109.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_2302;97_32659;98_41886;99_59109&stattab=map
- Last taxonomy: Cellulomonadaceae
- 16S sequence: KF056990
- Sequence Identity:
- Total samples: 1572
- soil counts: 388
- aquatic counts: 398
- animal counts: 641
- plant counts: 145
Sequence information
16S sequences
- @ref: 25310
- description: 16S rRNA gene sequences
- accession: KF056990
- length: 1463
- database: ena
- NCBI tax ID: 1348467
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Actinotalea caeni strain JCM 30447 | 1348467.3 | wgs | patric | 1348467 |
66792 | Actinotalea caeni JCM 30447 | 2890385099 | draft | img | 1348467 |
67770 | Actinotalea caeni JCM 30447 | GCA_009733795 | contig | ncbi | 1348467 |
GC content
- @ref: 25310
- GC-content: 74.8
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | yes | 90 | no |
motile | no | 61.336 | yes |
gram-positive | yes | 83.268 | no |
anaerobic | no | 99.092 | no |
halophile | no | 96.259 | no |
spore-forming | no | 89.145 | yes |
thermophile | no | 96.565 | yes |
glucose-util | yes | 85.604 | no |
aerobic | yes | 93.725 | no |
flagellated | no | 95.545 | no |
glucose-ferment | no | 84.112 | no |
External links
@ref: 25310
culture collection no.: KCTC 33604, JCM 30447
literature
- topic: Phylogeny
- Pubmed-ID: 28036250
- title: Actinotalea caeni sp. nov., isolated from a sludge sample of a biofilm reactor.
- authors: Jin L, Ko SR, Lee CS, Ahn CY, Lee JS, Lee KC, Oh HM, Lee HG
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001769
- year: 2017
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, *Biofilms, Bioreactors/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/chemistry
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | journal | pubmed |
---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||
25310 | L. K. Jin, S. R.,Lee, C. S.,Ahn, C. Y.,Lee, J. S.,Lee, K. C.,Oh, H. M.,Lee, H. G. | Actinotalea caeni sp. nov., isolated from a sludge sample of a biofilm reactor | 10.1099/ijsem.0.001769 | IJSEM 67: 1595-1599 2017 | 28036250 |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 |