Strain identifier

BacDive ID: 133103

Type strain: Yes

Species: Subtercola lobariae

Strain Designation: 9583b

Culture col. no.: DSM 103962, CGMCC 1.12976, KCTC 33586

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General

@ref: 64131

BacDive-ID: 133103

DSM-Number: 103962

keywords: gram-positive, aerobe, Bacteria, 16S sequence, rod-shaped, colony-forming

description: Subtercola lobariae 9583b is an aerobe, gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from specimens of the lichen Lobaria retigera collected from Jiaozi Snow Mountain, Yunnan Province.

strain history: <- H.-L. Si, Shandong Univ., Jinan, China; 9583b

doi: 10.13145/bacdive133103.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Subtercola
  • species: Subtercola lobariae
  • full scientific name: Subtercola lobariae Si et al. 2017

@ref: 64131

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Subtercola

species: Subtercola lobariae

full scientific name: Subtercola lobariae Si et al. 2017

strain designation: 9583b

type strain: yes

Morphology

cell morphology

  • @ref: 25307
  • gram stain: positive
  • cell length: 0.7-1.0 µm
  • cell width: 0.5-0.6 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 25307
  • colony size: 3-5 mm
  • colony color: white
  • colony shape: circular
  • incubation period: 3 days
  • medium used: ISP2

Culture and growth conditions

culture medium

@refnamegrowthlink
25307ISP2yes
25307R2A mediumyeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830.pdf
25307GYM STREPTOMYCES MEDIUM (DSMZ Medium 65), 20°Cyeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf
64131R2A MEDIUM (DSMZ Medium 830)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830.pdf
64131GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf

culture temp

@refgrowthtypetemperaturerange
25307positivegrowth4-28
25307positiveoptimum20psychrophilic
64131positivegrowth20psychrophilic

culture pH

@refabilitytypepH
25307positivegrowth4.0-8.0
25307positiveoptimum6

Physiology and metabolism

oxygen tolerance

  • @ref: 25307
  • oxygen tolerance: aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2530717128adipate-assimilation
2530717968butyrate-assimilation
2530785146carboxymethylcellulose-hydrolysis
25307casein-hydrolysis
2530716947citrate-assimilation
2530718333D-arabitol-respiration
253078391D-gluconate-respiration
2530716991dna-hydrolysis
2530724265gluconate-assimilation
2530725115malate-assimilation
2530717306maltose-assimilation
2530729864mannitol-assimilation
25307506227N-acetylglucosamine-assimilation
2530717632nitrate-reduction
2530728017starch-hydrolysis
2530727082trehalose-respiration
2530717925alpha-D-glucose+respiration
2530722599arabinose+assimilation
2530717057cellobiose+respiration
2530712936D-galactose+respiration
2530716024D-mannose+respiration
2530723652dextrin+respiration
253074853esculin+hydrolysis
2530728066gentiobiose+respiration
2530717234glucose+builds acid from
2530717234glucose+assimilation
2530717754glycerol+respiration
2530717268myo-inositol+respiration
2530737684mannose+assimilation
2530728053melibiose+respiration
2530718401phenylacetate+assimilation
2530717814salicin+respiration
2530753426tween 80+/-hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
25307100147nalidixic acidyesyes
25307161680aztreonamyesyes
2530775248potassium telluriteyesyes
253071 % sodium lactateyesyes
2530771321fusidateyesyes
2530716523d-serineyesyes
2530729673rifamycin svyesyes
2530750694minocyclineyesyes
253076472lincomycinyesyes
2530728001vancomycinyesyes
2530732735guanidinium chlorideyesyes
2530775273niaproofyesyes
2530775193tetrazolium violetyesyes
2530748607lithium chlorideyesyes
2530764103sodium butyrateyesyes
2530775229sodium bromateyesyes

metabolite production

  • @ref: 25307
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
25307acid phosphatase+3.1.3.2
25307alpha-galactosidase-3.2.1.22
25307alpha-glucosidase+3.2.1.20
25307alpha-mannosidase+3.2.1.24
25307arginine dihydrolase-3.5.3.6
25307beta-galactosidase+3.2.1.23
25307beta-glucosidase+3.2.1.21
25307catalase+1.11.1.6
25307cystine arylamidase+/-3.4.11.3
25307cytochrome oxidase-1.9.3.1
25307esterase Lipase (C 8)+/-
25307gelatinase-
25307leucine arylamidase+3.4.11.1
25307N-acetyl-beta-glucosaminidase-3.2.1.52
25307naphthol-AS-BI-phosphohydrolase+
25307trypsin+3.4.21.4
25307urease-3.5.1.5
25307valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25307C15:0 anteiso68.8
    25307C17:0 anteiso4.2
    25307C14:0 2OH10.3
    25307C17:1ω9c3.5
    25307C14:0 iso 3OH2.3
    25307C15:0 iso1.4
    25307C16:0 iso6.7
  • type of FA analysis: whole cell analysis
  • incubation medium: ISP2
  • agar/liquid: liquid
  • incubation temperature: 25
  • incubation time: 2
  • software version: Sherlock 4.5
  • library/peak naming table: TSBA 40
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeisolation date
25307specimens of the lichen Lobaria retigera collected from Jiaozi Snow Mountain, Yunnan ProvinceJiaozi Snow Mountain, Yunnan ProvinceChinaCHNAsia251022012-11
64131lichen Lobaria retigeraYunnan Province, Jiaozi Snow Mountain (25.86° N 102.81°E)ChinaCHNAsia26.86102.81

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Lichen

Safety information

risk assessment

  • @ref: 64131
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 64131
  • description: Subtercola lobariae strain 9583b 16S ribosomal RNA gene, partial sequence
  • accession: KM924549
  • length: 1460
  • database: ena
  • NCBI tax ID: 1588641

GC content

  • @ref: 64131
  • GC-content: 66.8
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 64131

culture collection no.: DSM 103962, CGMCC 1.12976, KCTC 33586

Reference

@idauthorstitledoi/urljournalcatalogue
20215D.Gleim, M.Kracht, N.Weiss et. al.Prokaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes sincehttp://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.html
25307H. L. S. Si, F. X.,Zhang, L. L.,Yue, H. S.,Wang, H. Y.,Zhao, Z. T.Subtercola lobariae sp. nov., an actinobacterium of the family Microbacteriaceae isolated from the lichen Lobaria retigera10.1099/ijsem.0.001753IJSEM 67: 1516-1521 2017
64131Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103962Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103962)