Strain identifier

BacDive ID: 133084

Type strain: Yes

Species: Macrococcus canis

Strain Designation: KM 45013

Strain history: CIP <- 2017, DSMZ <- Bern Univ., Bern, Switzerland: strain KM 45013

NCBI tax ID(s): 1855823 (species)

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General

@ref: 24872

BacDive-ID: 133084

DSM-Number: 101690

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, psychrophilic, Gram-positive, rod-shaped, colony-forming

description: Macrococcus canis KM 45013 is a facultative anaerobe, psychrophilic, Gram-positive bacterium that forms circular colonies and was isolated from nose of Bernese mountain dog with mucopurulent rhinitis.

NCBI tax id

  • NCBI tax id: 1855823
  • Matching level: species

strain history

@refhistory
24872<- V. Perreten, Univ. Berne, Inst. of Vet. Bacteriol., Switzerland; KM45013 <- A. Thomann
117136CIP <- 2017, DSMZ <- Bern Univ., Bern, Switzerland: strain KM 45013

doi: 10.13145/bacdive133084.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Macrococcus
  • species: Macrococcus canis
  • full scientific name: Macrococcus canis Gobeli Brawand et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Macrococcoides canis

@ref: 24872

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Macrococcus

species: Macrococcus canis

full scientific name: Macrococcus canis Gobeli Brawand et al. 2017

strain designation: KM 45013

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotility
25240positive0.8 µmrod-shapedno
117136positivecoccus-shapedno

colony morphology

  • @ref: 25240
  • type of hemolysis: CAMP test
  • hemolysis ability: 1
  • colony size: 2 mm
  • colony color: greyish white
  • colony shape: circular
  • incubation period: 1 day
  • medium used: TSA-SB agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25240TSA-SB agaryes
24872TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
24872COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
117136CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
25240positivegrowth18psychrophilic
25240positivegrowth37mesophilic
25240positivegrowth43thermophilic
63530positivegrowth37mesophilic
24872positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
25240facultative anaerobe
63530aerobe

antibiogram

@refmediumincubation temperatureoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
24872Mueller-Hinton Agar37aerob16-181016-1818-201828-3024-26160444034-3634-262622322230301016-182620142822-244010303630-3238-40026-281822
24872Mueller-Hinton Agar37aerob16-181016-1818-201828-3024-26160444034-3634-262622322230301016-182620142822-244010303630-3238-40026-281822

halophily

@refsaltgrowthtested relationconcentration
25240NaClpositivegrowth9 %
25240NaClpositivegrowth12 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2524017019(R)-amygdalin-assimilation
2524023456cyclodextrin-assimilation
2524012936D-galactose-builds acid from
2524016988D-ribose-builds acid from
2524017924D-sorbitol-builds acid from
2524065327D-xylose-builds acid from
2524017716lactose-builds acid from
2524016634raffinose-builds acid from
2524017814salicin-assimilation
2524017992sucrose-builds acid from
2524016899D-mannitol+builds acid from
2524016024D-mannose+builds acid from
2524017306maltose+builds acid from
2524027082trehalose+builds acid from
6837516199urea-hydrolysis
6837529016arginine-hydrolysis
6837518257ornithine-degradation
683754853esculin-hydrolysis
6837517634D-glucose-fermentation
6837515824D-fructose+fermentation
6837516024D-mannose-fermentation
6837517306maltose-fermentation
6837517716lactose-fermentation
6837527082trehalose-fermentation
6837516899D-mannitol-fermentation
6837516634raffinose-fermentation
6837516988D-ribose-fermentation
6837517057cellobiose-fermentation
6837517632nitrate+reduction
6837517992sucrose-fermentation
6837559640N-acetylglucosamine-fermentation
6837532528turanose-fermentation
6837522599arabinose-fermentation

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitivesensitivity conc.
2524086455optochinyesyes
2524073908vibriostatyesyes
2524028368novobiocinyesyes
252408309polymyxin byesno
2524028669bacitracinyesno
6837528368novobiocinyesyes1.8 µg

metabolite production

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
25240alanine arylamidase-3.4.11.2
25240alpha-galactosidase-3.2.1.22
25240alpha-glucosidase+3.2.1.20
25240alpha-mannosidase-3.2.1.24
25240arginine dihydrolase+3.5.3.6
25240arginine dihydrolase-3.5.3.6
25240beta-galactopyranosidase-
25240beta-galactosidase-3.2.1.23
25240beta-glucuronidase-3.2.1.31
25240catalase+1.11.1.6
25240cytochrome oxidase+1.9.3.1
25240DNase+
25240L-aspartate arylamidase-3.4.11.21
25240leucine arylamidase+3.4.11.1
25240phosphatase-
25240phosphatidylinositol phospholipase C-3.1.4.11
25240proline-arylamidase-3.4.11.5
25240pyrrolidonyl arylamidase+3.4.19.3
25240tripeptide aminopeptidase-3.4.11.4
25240tyrosine arylamidase+
25240urease-3.5.1.5
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase-3.4.19.3
68375alkaline phosphatase-3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase-3.5.3.6
68375urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25240C14:029.5
    25240C16:05
    25240C16:0 n alcohol20.7
    25240C16:1ω11c18.4
    25240C18:01.5
    25240C18:3ω6c (6,9,12)22.3
    25240C20:00.7
    25240C12:0 iso0.9
    25240C14:0 iso1.2
  • type of FA analysis: whole cell analysis
  • incubation medium: trypticase soy broth
  • agar/liquid: agar
  • incubation temperature: 28
  • incubation time: 1
  • system: MIS MIDI
  • method/protocol: Miller 1982
  • cutoff value:

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
24872-----+--------++----------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperaturesampling dategeographic location
24872nose of Bernese mountain dog with mucopurulent rhinitisSwitzerlandCHEEurope
25240trypticase soy agarcontaining 5 % sheep blood (TSA-SB; Becton Dickinson)1 day37
63530Sinusitis,Bernese Mountain dogSwitzerlandCHEEurope2013-04-30Bern
117136Animal, Bernese mountain dog, mucopurulent rhinitisSwitzerlandCHEEuropeBern

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Host#Mammals#Canidae (Dog)
#Host Body-Site#Organ#Nose

taxonmaps

  • @ref: 69479
  • File name: preview.99_2313.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_1248;97_1481;98_1805;99_2313&stattab=map
  • Last taxonomy: Macrococcus
  • 16S sequence: KU933342
  • Sequence Identity:
  • Total samples: 10750
  • soil counts: 1069
  • aquatic counts: 930
  • animal counts: 8379
  • plant counts: 372

Safety information

risk assessment

  • @ref: 117136
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 24872
  • description: Macrococcus canis strain KM 45013 16S ribosomal RNA gene, partial sequence
  • accession: KU933342
  • length: 1547
  • database: ena
  • NCBI tax ID: 1855823

Genome sequences

  • @ref: 66792
  • description: Macrococcus canis KM 45013
  • accession: GCA_002119805
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 1855823

GC content

  • @ref: 24872
  • GC-content: 36.9
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno92.07yes
gram-positiveyes93.802yes
anaerobicno98.704no
halophileyes87.157yes
spore-formingno78.565no
glucose-utilyes88.419no
aerobicyes88.091no
thermophileno99.855no
glucose-fermentno73.346no
motileno88.685yes

External links

@ref: 24872

culture collection no.: DSM 101690, CCOS 969, CCUG 68920, CIP 111363

straininfo link

  • @ref: 91349
  • straininfo: 405974

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27902286Macrococcus canis sp. nov., a skin bacterium associated with infections in dogs.Gobeli Brawand S, Cotting K, Gomez-Sanz E, Collaud A, Thomann A, Brodard I, Rodriguez-Campos S, Strauss C, Perreten VInt J Syst Evol Microbiol10.1099/ijsem.0.0016732017Animals, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Dog Diseases/*microbiology, Dogs/*microbiology, Fatty Acids/chemistry, Genes, Bacterial, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Skin/*microbiology, Skin Diseases, Bacterial/*veterinary, Staphylococcaceae/*classification/genetics/isolation & purification, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Genetics29348359Complete Genome Sequence of the Type Strain of Macrococcus canis.Gobeli Brawand S, Rychener L, Schwendener S, Pantucek R, Perreten VGenome Announc10.1128/genomeA.01507-172018
Phylogeny35156917Macrococcus armenti sp. nov., a novel bacterium isolated from the skin and nasal cavities of healthy pigs and calves.Keller JE, Schwendener S, Overesch G, Perreten VInt J Syst Evol Microbiol10.1099/ijsem.0.0052452022Animals, Bacterial Typing Techniques, Base Composition, Cattle/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, *Nasal Cavity/microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Skin/*microbiology, Staphylococcaceae/*classification/isolation & purification, Swine/*microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24872Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101690Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101690)
25240S. C. Gobeli Brawand, Kerstin,Gómez-Sanz, Elena,Collaud, Alexandra,Thomann, Andreas,Brodard, Isabelle,Rodriguez-Campos, Sabrina,Strauss, Christian,Perreten, VincentMacrococcus canis sp. nov., a skin bacterium associated with infections in dogs10.1099/ijsem.0.001673IJSEM 67: 621-626 201727902286
63530Curators of the CCUGhttps://www.ccug.se/strain?id=68920Culture Collection University of Gothenburg (CCUG) (CCUG 68920)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68375Automatically annotated from API ID32STA
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
91349Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405974.1
117136Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20111363Collection of Institut Pasteur (CIP 111363)