Strain identifier

BacDive ID: 133077

Type strain: Yes

Species: Racemicystis persica

Strain Designation: 37

Strain history: <- J. Wink, HZI, Braunschweig, Germany <- A. Moradi, Faculty of Biological Sciences, Iran

NCBI tax ID(s): 1885912 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 24865

BacDive-ID: 133077

DSM-Number: 103165

keywords: Gram-negative, mesophilic, spore-forming, chemoheterotroph, aerobe, Bacteria, 16S sequence, motile, rod-shaped

description: Racemicystis persica 37 is an aerobe, chemoheterotroph, spore-forming bacterium that builds fruiting-like bodies and was isolated from beach sand.

NCBI tax id

  • NCBI tax id: 1885912
  • Matching level: species

strain history: <- J. Wink, HZI, Braunschweig, Germany <- A. Moradi, Faculty of Biological Sciences, Iran

doi: 10.13145/bacdive133077.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Myxococcales
  • family: Polyangiaceae
  • genus: Racemicystis
  • species: Racemicystis persica
  • full scientific name: Racemicystis persica Moradi et al. 2017

@ref: 24865

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Polyangiaceae

genus: Racemicystis

species: Racemicystis persica

full scientific name: Racemicystis persica Moradi et al. 2017

strain designation: 37

type strain: yes

Morphology

cell morphology

  • @ref: 25205
  • gram stain: negative
  • cell length: 3.0-12.0 µm
  • cell width: 1.2-3.0 µm
  • cell shape: rod-shaped
  • motility: yes

colony morphology

  • @ref: 25205
  • colony color: orange to beige

multicellular morphology

  • @ref: 25205
  • forms multicellular complex: yes
  • complex name: fruiting-like bodies
  • complex size: 200.0-800.0 µm

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
25205medium CYyes
25205medium Hyes
25205medium Eyes
25205medium Kyes
25205VY/2 modifiedyes80 % VY/2, 10 % CY, 10 % S-medium, vitamin solution and additional vitamin B 12
24865VY/2 AGAR (DSMZ Medium 9)yesName: VY/2 AGAR (DSMZ Medium 9) Composition: Agar 15.0 g/l Baker's yeast 5.0 g/l CaCl2 x 2 H2O 1.36 g/l Vitamin B12 0.0005 g/l Distilled waterhttps://bacmedia.dsmz.de/medium/9

culture temp

@refrangegrowthtypetemperature
25205mesophilic
24865mesophilicpositivegrowth28

culture pH

@refabilitytypepH
25205positiveoptimum5.0-6.0
25205positiveminimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 25205
  • oxygen tolerance: aerobe

nutrition type

  • @ref: 25205
  • type: chemoheterotroph

spore formation

  • @ref: 25205
  • spore description: 1.2-1.5 x 3.0-8.0 µm with blunted ends
  • type of spore: myxospore
  • spore formation: yes

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantis sensitive
2520517833gentamicinyesyes
2520559062polymyxinyesyes
2520571321fusidateyesyes
2520545924trimethoprimyesyes
2520528971ampicillinyesyes
252056104kanamycinyesyes
252059215spectinomycinyesyes
2520523066cephalosporinyesyes
2520517698chloramphenicolyesyes
2520527701oxytetracyclineyesyes
2520524753hygromycinyesyes
2520529693thiostreptonyesyes
25205bacillomycinyesyes

enzymes

@refvalueactivityec
25205acid phosphatase+3.1.3.2
25205alkaline phosphatase+3.1.3.1
25205alpha-chymotrypsin+3.4.21.1
25205alpha-fucosidase-3.2.1.51
25205alpha-galactosidase-3.2.1.22
25205alpha-glucosidase+3.2.1.20
25205alpha-mannosidase-3.2.1.24
25205beta-galactosidase-3.2.1.23
25205beta-glucosidase+3.2.1.21
25205beta-glucuronidase-3.2.1.31
25205cystine arylamidase+3.4.11.3
25205esterase (C 4)+
25205esterase Lipase (C 8)+
25205leucine arylamidase+3.4.11.1
25205lipase (C 14)+
25205N-acetyl-beta-glucosaminidase-3.2.1.52
25205naphthol-AS-BI-phosphohydrolase+
25205trypsin-3.4.21.4
25205valine arylamidase+

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    25205C15:0 anteiso0.1
    25205C17:0 anteiso0.4
    25205C14:00.4
    25205C14:0 OAG0.9
    25205C15:00.2
    25205C15:0 OAG0.3
    25205C16:05.3
    25205C16:0 OAG2.9
    25205C16:14.6
    25205C16:1 2OH0.5
    25205C16:1 OAG7.7
    25205C17:00.8
    25205C17:0 OAG0.4
    25205C17:10.5
    25205C18:04.3
    25205C18:0 2OH0.3
    25205C18:18.1
    25205C18:1 2OH9.6
    25205C18:21
    25205C19:00.1
    25205C26:00.4
    25205C13:0 iso0.2
    25205C14:0 iso0.1
    25205C15:0 iso11
    25205iso-C15:0 OAG7.8
    25205C15:1 iso0.1
    25205C16:0 iso1.1
    25205iso-C16:0 OAG0.3
    25205C17:0 iso17.4
    25205C17:0 iso 2OH0.1
    25205iso-C17:0 OAG1.8
    25205C17:1 iso3.6
    25205iso-C17:1 2-OH5.7
    25205iso-C17:1 OAG0.8
    25205iso-C18:1 2-OH0.7
    25205iso-C19:0 2-OH0.1
    25205iso-C19:1 2-OH0.3
  • type of FA analysis: whole cell analysis
  • incubation medium: M medium per litre: 10 g soy peptone, 10 g maltose, 1 g CaCl2 , 1 g MgSO4 , 11.9 g HEPES, 80 mg Fe-EDTA, pH 7.2
  • incubation temperature: 30
  • incubation time: 5
  • treatment: 180 r.p.m.
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudesampling dateenrichment culture
24865beach sandPersian Gulf, Kish Island beachIranIRNAsia26.557254.0243
25205Kish Island beach sand2015buffered VY/2 and CY agar

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Coast
#Environmental#Terrestrial#Sandy

Safety information

risk assessment

  • @ref: 24865
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24865
  • description: Racemicystis persica strain MSr11462 16S ribosomal RNA gene, partial sequence
  • accession: KX443485
  • length: 1505
  • database: ena
  • NCBI tax ID: 1885912

External links

@ref: 24865

culture collection no.: DSM 103165, NCCB 100606, MSR MSr11462, MSR 11462

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902273
  • title: Racemicystis persica sp. nov., a myxobacterium from soil.
  • authors: Moradi A, Ebrahimipour GH, Mohr KI, Kampfer P, Glaeser SP, Hennessen F, Gemperlein K, Awal RP, Wolf C, Muller R, Wink J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001655
  • year: 2017
  • mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Iran, Islands, Myxococcales/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24865Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-103165Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103165)
25205A. E. Moradi, G. H.,Mohr, K. I.,Kämpfer, P.,Glaeser, S. P.,Hennessen, F.,Gemperlein, K.,Awal, R. P.,Wolf, C.,Müller, R.,Wink, J.Racemicystis persica sp. nov., a myxobacterium from soil10.1099/ijsem.0.001655IJSEM 67: 472-478 201727902273