Strain identifier
BacDive ID: 133077
Type strain:
Species: Racemicystis persica
Strain Designation: 37
Strain history: <- J. Wink, HZI, Braunschweig, Germany <- A. Moradi, Faculty of Biological Sciences, Iran
NCBI tax ID(s): 1885912 (species)
General
@ref: 24865
BacDive-ID: 133077
DSM-Number: 103165
keywords: 16S sequence, Bacteria, aerobe, chemoheterotroph, spore-forming, mesophilic, Gram-negative, motile, rod-shaped
description: Racemicystis persica 37 is an aerobe, chemoheterotroph, spore-forming bacterium that builds fruiting-like bodies and was isolated from beach sand.
NCBI tax id
- NCBI tax id: 1885912
- Matching level: species
strain history
- @ref: 24865
- history: <- J. Wink, HZI, Braunschweig, Germany <- A. Moradi, Faculty of Biological Sciences, Iran
doi: 10.13145/bacdive133077.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Myxococcales
- family: Polyangiaceae
- genus: Racemicystis
- species: Racemicystis persica
- full scientific name: Racemicystis persica Moradi et al. 2017
@ref: 24865
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Myxococcales
family: Polyangiaceae
genus: Racemicystis
species: Racemicystis persica
full scientific name: Racemicystis persica Moradi et al. 2017
strain designation: 37
type strain: yes
Morphology
cell morphology
- @ref: 25205
- gram stain: negative
- cell length: 3.0-12.0 µm
- cell width: 1.2-3.0 µm
- cell shape: rod-shaped
- motility: yes
colony morphology
- @ref: 25205
- colony color: orange to beige
multicellular morphology
- @ref: 25205
- forms multicellular complex: yes
- complex name: fruiting-like bodies
- complex size: 200.0-800.0 µm
Culture and growth conditions
culture medium
@ref | name | growth | composition | link |
---|---|---|---|---|
25205 | medium CY | yes | ||
25205 | medium H | yes | ||
25205 | medium E | yes | ||
25205 | medium K | yes | ||
25205 | VY/2 modified | yes | 80 % VY/2, 10 % CY, 10 % S-medium, vitamin solution and additional vitamin B 12 | |
24865 | VY/2 AGAR (DSMZ Medium 9) | yes | Name: VY/2 AGAR (DSMZ Medium 9) Composition: Agar 15.0 g/l Baker's yeast 5.0 g/l CaCl2 x 2 H2O 1.36 g/l Vitamin B12 0.0005 g/l Distilled water | https://mediadive.dsmz.de/medium/9 |
culture temp
@ref | range | growth | type | temperature |
---|---|---|---|---|
25205 | mesophilic | |||
24865 | mesophilic | positive | growth | 28 |
culture pH
@ref | ability | type | pH |
---|---|---|---|
25205 | positive | optimum | 5.0-6.0 |
25205 | positive | minimum | 7 |
Physiology and metabolism
oxygen tolerance
- @ref: 25205
- oxygen tolerance: aerobe
nutrition type
- @ref: 25205
- type: chemoheterotroph
spore formation
- @ref: 25205
- spore description: 1.2-1.5 x 3.0-8.0 µm with blunted ends
- type of spore: myxospore
- spore formation: yes
antibiotic resistance
@ref | ChEBI | metabolite | is antibiotic | is resistant | is sensitive |
---|---|---|---|---|---|
25205 | 17833 | gentamicin | yes | yes | |
25205 | 59062 | polymyxin | yes | yes | |
25205 | 71321 | fusidate | yes | yes | |
25205 | 45924 | trimethoprim | yes | yes | |
25205 | 28971 | ampicillin | yes | yes | |
25205 | 6104 | kanamycin | yes | yes | |
25205 | 9215 | spectinomycin | yes | yes | |
25205 | 23066 | cephalosporin | yes | yes | |
25205 | 17698 | chloramphenicol | yes | yes | |
25205 | 27701 | oxytetracycline | yes | yes | |
25205 | 24753 | hygromycin | yes | yes | |
25205 | 29693 | thiostrepton | yes | yes | |
25205 | bacillomycin | yes | yes |
enzymes
@ref | value | activity | ec |
---|---|---|---|
25205 | acid phosphatase | + | 3.1.3.2 |
25205 | alkaline phosphatase | + | 3.1.3.1 |
25205 | alpha-chymotrypsin | + | 3.4.21.1 |
25205 | alpha-fucosidase | - | 3.2.1.51 |
25205 | alpha-galactosidase | - | 3.2.1.22 |
25205 | alpha-glucosidase | + | 3.2.1.20 |
25205 | alpha-mannosidase | - | 3.2.1.24 |
25205 | beta-galactosidase | - | 3.2.1.23 |
25205 | beta-glucosidase | + | 3.2.1.21 |
25205 | beta-glucuronidase | - | 3.2.1.31 |
25205 | cystine arylamidase | + | 3.4.11.3 |
25205 | esterase (C 4) | + | |
25205 | esterase Lipase (C 8) | + | |
25205 | leucine arylamidase | + | 3.4.11.1 |
25205 | lipase (C 14) | + | |
25205 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
25205 | naphthol-AS-BI-phosphohydrolase | + | |
25205 | trypsin | - | 3.4.21.4 |
25205 | valine arylamidase | + |
fatty acid profile
fatty acids
@ref fatty acid percentage 25205 C15:0 anteiso 0.1 25205 C17:0 anteiso 0.4 25205 C14:0 0.4 25205 C14:0 OAG 0.9 25205 C15:0 0.2 25205 C15:0 OAG 0.3 25205 C16:0 5.3 25205 C16:0 OAG 2.9 25205 C16:1 4.6 25205 C16:1 2OH 0.5 25205 C16:1 OAG 7.7 25205 C17:0 0.8 25205 C17:0 OAG 0.4 25205 C17:1 0.5 25205 C18:0 4.3 25205 C18:0 2OH 0.3 25205 C18:1 8.1 25205 C18:1 2OH 9.6 25205 C18:2 1 25205 C19:0 0.1 25205 C26:0 0.4 25205 C13:0 iso 0.2 25205 C14:0 iso 0.1 25205 C15:0 iso 11 25205 iso-C15:0 OAG 7.8 25205 C15:1 iso 0.1 25205 C16:0 iso 1.1 25205 iso-C16:0 OAG 0.3 25205 C17:0 iso 17.4 25205 C17:0 iso 2OH 0.1 25205 iso-C17:0 OAG 1.8 25205 C17:1 iso 3.6 25205 iso-C17:1 2-OH 5.7 25205 iso-C17:1 OAG 0.8 25205 iso-C18:1 2-OH 0.7 25205 iso-C19:0 2-OH 0.1 25205 iso-C19:1 2-OH 0.3 - type of FA analysis: whole cell analysis
- incubation medium: M medium per litre: 10 g soy peptone, 10 g maltose, 1 g CaCl2 , 1 g MgSO4 , 11.9 g HEPES, 80 mg Fe-EDTA, pH 7.2
- incubation temperature: 30
- incubation time: 5
- treatment: 180 r.p.m.
- system: MIS MIDI
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | sampling date | enrichment culture |
---|---|---|---|---|---|---|---|---|---|
24865 | beach sand | Persian Gulf, Kish Island beach | Iran | IRN | Asia | 26.5572 | 54.0243 | ||
25205 | Kish Island beach sand | 2015 | buffered VY/2 and CY agar |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Terrestrial | #Coast |
#Environmental | #Terrestrial | #Sandy |
Safety information
risk assessment
- @ref: 24865
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24865
- description: Racemicystis persica strain MSr11462 16S ribosomal RNA gene, partial sequence
- accession: KX443485
- length: 1505
- database: ena
- NCBI tax ID: 1885912
External links
@ref: 24865
culture collection no.: DSM 103165, NCCB 100606, MSR MSr11462, MSR 11462
straininfo link
- @ref: 91342
- straininfo: 398271
literature
- topic: Phylogeny
- Pubmed-ID: 27902273
- title: Racemicystis persica sp. nov., a myxobacterium from soil.
- authors: Moradi A, Ebrahimipour GH, Mohr KI, Kampfer P, Glaeser SP, Hennessen F, Gemperlein K, Awal RP, Wolf C, Muller R, Wink J
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001655
- year: 2017
- mesh: Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Iran, Islands, Myxococcales/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
24865 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-103165 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 103165) | |||
25205 | A. E. Moradi, G. H.,Mohr, K. I.,Kämpfer, P.,Glaeser, S. P.,Hennessen, F.,Gemperlein, K.,Awal, R. P.,Wolf, C.,Müller, R.,Wink, J. | Racemicystis persica sp. nov., a myxobacterium from soil | 10.1099/ijsem.0.001655 | IJSEM 67: 472-478 2017 | 27902273 | |
91342 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID398271.1 |