Strain identifier

BacDive ID: 133069

Type strain: Yes

Species: Lactobacillus colini

Strain Designation: L1, 111144-L1, L1(historical number)

Strain history: <- Shuping Zhang, Univ. of Missouri <- S Zhang and H Su, Univ. Texas

NCBI tax ID(s): 1819254 (species)

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General

@ref: 24857

BacDive-ID: 133069

DSM-Number: 101872

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-positive, rod-shaped, colony-forming

description: Lactobacillus colini L1 is a facultative anaerobe, Gram-positive, rod-shaped bacterium that forms circular colonies and was isolated from intestinal contents of a Northern Bobwhite .

NCBI tax id

  • NCBI tax id: 1819254
  • Matching level: species

strain history

@refhistory
24857<- S. Zhang, Univ. Missouri, USA <- S. Zhang and H. Su, Univ. Texas
67771<- Shuping Zhang, Univ. of Missouri <- S Zhang and H Su, Univ. Texas

doi: 10.13145/bacdive133069.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Lactobacillus
  • species: Lactobacillus colini
  • full scientific name: Lactobacillus colini Zhang et al. 2017

@ref: 24857

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Lactobacillus

species: Lactobacillus colini

full scientific name: Lactobacillus colini Zhang et al. 2017

strain designation: L1, 111144-L1, L1(historical number)

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
25191positiverod-shapedno
67771rod-shapedno
67771positive
69480no94.643
69480positive99.731

colony morphology

@reftype of hemolysishemolysis abilitycolony sizecolony colorcolony shapeincubation periodmedium used
25191alpha10.5-1.5 mmgreycircular2 daysColumbia blood agar
251911.0-1.5 mmirregular2 daysMRS agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25191Columbia blood agaryes
25191MRS agaryes
25191BHI brothyes
25191MRS brothyes
24857MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
25191positivegrowth25.0-45.0
25191positiveoptimum37mesophilic
25191nogrowth20psychrophilic
24857positivegrowth37mesophilic
67771positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
25191positivegrowth3.5-10.0alkaliphile
25191positiveoptimum5.0-5.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
25191facultative anaerobe
67771microaerophile

spore formation

@refspore formationconfidence
25191no
67771no
69481no100
69480no99.98

halophily

@refsaltgrowthtested relationconcentration
25191NaClpositivegrowth0.5-3.0 %
25191NaCloptimum<1.0 %
25191NaClnogrowth4 %

murein

  • @ref: 24857
  • murein short key: A11.31
  • type: A4alpha L-Lys-D-Asp

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2519116443D-tagatose-builds acid from
2519128066gentiobiose-builds acid from
2519117716lactose-builds acid from
2519127613amygdalin+builds acid from
2519127613amygdalin+hydrolysis
2519118305arbutin+hydrolysis
2519117057cellobiose+builds acid from
2519115824D-fructose+builds acid from
2519112936D-galactose+builds acid from
2519117634D-glucose+builds acid from
2519116899D-mannitol+builds acid from
2519116024D-mannose+builds acid from
2519116988D-ribose+builds acid from
251914853esculin+hydrolysis
2519117306maltose+builds acid from
251916731melezitose+builds acid from
25191320061methyl alpha-D-glucopyranoside+builds acid from
25191320061methyl alpha-D-glucopyranoside+hydrolysis
25191506227N-acetylglucosamine+builds acid from
25191506227N-acetylglucosamine+hydrolysis
2519116634raffinose+builds acid from
2519117814salicin+builds acid from
2519128017starch+hydrolysis
2519117992sucrose+builds acid from
2519127082trehalose+builds acid from
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose+builds acid from
683716731melezitose+builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from

enzymes

@refvalueactivityec
25191alpha-galactosidase+3.2.1.22
25191alpha-glucosidase+3.2.1.20
25191beta-glucosidase+3.2.1.21
25191catalase-1.11.1.6
25191cytochrome oxidase-1.9.3.1
25191leucine arylamidase+3.4.11.1
25191naphthol-AS-BI-phosphohydrolase-

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
24857-----+----++++/-----+--++++++++--++-++---+----------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
24857intestinal contents of a Northern Bobwhite (Colinus virginianus)Colinus virginianuswestern Texas, San AngeloUSAUSANorth America
25191Columbia blood agarwith 5 % sheep blood1 day37
25191anaerobic PEA agar plates1 day37
67771From the intestine of a Northern bobwhite quail, `Colinus virginianus`San Angelo, western TexasUSAUSANorth America

isolation source categories

Cat1Cat2
#Host#Birds
#Host Body-Site#Gastrointestinal tract

Safety information

risk assessment

  • @ref: 24857
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24857
  • description: Lactobacillus colini strain 111144-L1 16S ribosomal RNA gene, partial sequence
  • accession: KU161105
  • length: 1566
  • database: ena
  • NCBI tax ID: 1819254

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus colini DSM 101872GCA_017874575contigncbi1819254
66792Lactobacillus colini strain DSM 1018721819254.3wgspatric1819254
66792Lactobacillus colini DSM 1018722913564238draftimg1819254

GC content

@refGC-contentmethod
2485735.6high performance liquid chromatography (HPLC)
6777135.6

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno95.963yes
gram-positiveyes95.667no
anaerobicyes53.978yes
halophileyes70.081no
spore-formingno94.61no
glucose-utilyes92.988no
flagellatedno98.288yes
thermophileno97.901yes
aerobicno97.029yes
glucose-fermentyes91.823no

External links

@ref: 24857

culture collection no.: DSM 101872, KCTC 21086

straininfo link

  • @ref: 91334
  • straininfo: 396956

literature

  • topic: Phylogeny
  • Pubmed-ID: 27902241
  • title: Lactobacillus colini sp. nov., isolated from Northern Bobwhite (Colinus virginianus).
  • authors: Zhang MZ, Yang M, Su H, Rollins D, Zhang S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001624
  • year: 2017
  • mesh: Animals, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, Colinus/*microbiology, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gastrointestinal Contents/*microbiology, Lactobacillus/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Texas
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24857Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101872Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101872)
25191M. Z. Y. Zhang, M.,Su, H.,Rollins, D.,Zhang, S.Lactobacillus colini sp. nov., isolated from Northern Bobwhite (Colinus virginianus)10.1099/ijsem.0.001624IJSEM 67: 325-329 201727902241
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68371Automatically annotated from API 50CH acid
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
91334Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID396956.1