Strain identifier

BacDive ID: 133055

Type strain: Yes

Species: Granulicella acidiphila

Strain history: <- C. Falagan, Bangor Univ., Wales, U.K.; MCF40

NCBI tax ID(s): 1335628 (species)

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General

@ref: 24843

BacDive-ID: 133055

DSM-Number: 28996

keywords: 16S sequence, Bacteria

description: Granulicella acidiphila DSM 28996 is a bacterium that was isolated from acidic pit lake.

NCBI tax id

  • NCBI tax id: 1335628
  • Matching level: species

strain history

  • @ref: 24843
  • history: <- C. Falagan, Bangor Univ., Wales, U.K.; MCF40

doi: 10.13145/bacdive133055.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/acidobacteriota
  • domain: Bacteria
  • phylum: Acidobacteriota
  • class: Terriglobia
  • order: Terriglobales
  • family: Acidobacteriaceae
  • genus: Granulicella
  • species: Granulicella acidiphila
  • full scientific name: Granulicella acidiphila Falagán et al. 2017

@ref: 24843

domain: Bacteria

phylum: Acidobacteria

class: Acidobacteriia

order: Acidobacteriales

family: Acidobacteriaceae

genus: Granulicella

species: Granulicella acidiphila

full scientific name: Granulicella acidiphila Falagán et al. 2017

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 24843
  • name: GRANULICELLA PALUDICOLA MEDIUM (DSMZ Medium 1285)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1285
  • composition: Name: GRANULICELLA PALUDICOLA MEDIUM (DSMZ Medium 1285) Composition: Agar 15.0 g/l Fructose 0.5 g/l KH2PO4 0.1 g/l (NH4)2SO4 0.1 g/l Casamino acids 0.05 g/l Yeast extract 0.05 g/l MgSO4 x 7 H2O 0.04 g/l CaCl2 x 2 H2O 0.02 g/l Distilled water

culture temp

  • @ref: 24843
  • growth: positive
  • type: growth
  • temperature: 30

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin+hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase+3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase-3.1.3.1
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382alpha-chymotrypsin-3.4.21.1
68382naphthol-AS-BI-phosphohydrolase+
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase+3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase+3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
24843--+/-+/--+++/-+/--+/-++/-++-+--+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
24843-----+-+------------
24843+----+-+----------+--

Isolation, sampling and environmental information

isolation

  • @ref: 24843
  • sample type: acidic pit lake
  • geographic location: Iberian Pyrite Belt, Huelva, Herrerías mine, Guadiana pit lake
  • country: Spain
  • origin.country: ESP
  • continent: Europe
  • latitude: 37.785
  • longitude: -6.8253

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Mine
#Environmental#Aquatic#Lake (large)
#Condition#Acidic

taxonmaps

  • @ref: 69479
  • File name: preview.99_10284.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_236;96_5081;97_6138;98_7662;99_10284&stattab=map
  • Last taxonomy: Granulicella
  • 16S sequence: KF017281
  • Sequence Identity:
  • Total samples: 1188
  • soil counts: 1001
  • aquatic counts: 71
  • animal counts: 99
  • plant counts: 17

Safety information

risk assessment

  • @ref: 24843
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24843
  • description: Granulicella acidiphila strain MCF40 16S ribosomal RNA gene, partial sequence
  • accession: KF017281
  • length: 1212
  • database: nuccore
  • NCBI tax ID: 1335628

GC content

  • @ref: 24843
  • GC-content: 62.1
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24843

culture collection no.: DSM 28996, NCCB 100577, MCF 40

straininfo link

  • @ref: 91322
  • straininfo: 403105

literature

  • topic: Phylogeny
  • Pubmed-ID: 28229259
  • title: Acidicapsa ferrireducens sp. nov., Acidicapsa acidiphila sp. nov., and Granulicella acidiphila sp. nov.: novel acidobacteria isolated from metal-rich acidic waters.
  • authors: Falagan C, Foesel B, Johnson B
  • journal: Extremophiles
  • DOI: 10.1007/s00792-017-0916-4
  • year: 2017
  • mesh: Acidobacteria/classification/genetics/*isolation & purification/metabolism, Base Composition, Iron/analysis/*metabolism, Lakes/chemistry/microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Water Microbiology
  • topic2: Metabolism

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24843Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28996Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28996)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
91322Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403105.1