Strain identifier

BacDive ID: 133030

Type strain: Yes

Species: Marinitoga arctica

Strain Designation: 2PyrY55-1

Strain history: B. O. Steinsbu; Centre for Geobiol., Univ. of Bergen, Norway; 2PyrY55-1.

NCBI tax ID(s): 1862713 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 24818

BacDive-ID: 133030

DSM-Number: 29778

keywords: 16S sequence, Bacteria, anaerobe, heterotroph, thermophilic, Gram-negative, motile, rod-shaped

description: Marinitoga arctica 2PyrY55-1 is an anaerobe, heterotroph, thermophilic bacterium that was isolated from wall of an active high-temperature white smoker chimney, 700 m depth.

NCBI tax id

  • NCBI tax id: 1862713
  • Matching level: species

strain history

@refhistory
24818<- B. O. Steinsbu, Univ. Bergen, Norway; 2PyrY55-1
67770B. O. Steinsbu; Centre for Geobiol., Univ. of Bergen, Norway; 2PyrY55-1.

doi: 10.13145/bacdive133030.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/thermotogota
  • domain: Bacteria
  • phylum: Thermotogota
  • class: Thermotogae
  • order: Petrotogales
  • family: Petrotogaceae
  • genus: Marinitoga
  • species: Marinitoga arctica
  • full scientific name: Marinitoga arctica Steinsbu et al. 2016

@ref: 24818

domain: Bacteria

phylum: Thermotogae

class: Thermotogae

order: Thermotogales

family: Petrotogaceae

genus: Marinitoga

species: Marinitoga arctica

full scientific name: Marinitoga arctica Steinsbu et al. 2016

strain designation: 2PyrY55-1

type strain: yes

Morphology

cell morphology

  • @ref: 25114
  • gram stain: negative
  • cell length: 1.0-5.0 µm
  • cell width: 0.5-0.8 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: monotrichous, polar

Culture and growth conditions

culture medium

  • @ref: 24818
  • name: MARINITOGA HYDROGENITOLERANS MEDIUM (DSMZ Medium 904a)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/904a
  • composition: Name: MARINITOGA HYDROGENITOLERANS MEDIUM (DSMZ Medium 904a) Composition: Sea Salt 30.0 g/l MES 4.0 g/l D-Glucose 2.5 g/l Tryptone 1.0 g/l Yeast extract 1.0 g/l Na2S x 9 H2O 0.5 g/l L-Cysteine HCl x H2O 0.5 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
25114positivegrowth45.0-70.0thermophilic
25114positiveoptimum65thermophilic
24818positivegrowth65thermophilic
67770positivegrowth65thermophilic

culture pH

@refabilitytypepH
25114positivegrowth5.0-7.5
25114positiveoptimum5.5

Physiology and metabolism

oxygen tolerance

  • @ref: 25114
  • oxygen tolerance: anaerobe

nutrition type

  • @ref: 25114
  • type: heterotroph

halophily

@refsalttested relationconcentrationgrowth
25114NaCloptimum2.5 %
25114NaClgrowth1.5-5.5 %(w/v)positive

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2511430089acetate-assimilation
2511485146carboxymethylcellulose-assimilation
25114casamino acids-assimilation
25114casein-assimilation
2511417029chitin-assimilation
2511428260galactose-assimilation
2511417716lactose-assimilation
2511433942ribose-assimilation
2511417057cellobiose+assimilation
2511462968cellulose+assimilation
2511415356cysteine+electron acceptor
2511433403elemental sulfur+electron acceptor
2511428757fructose+assimilation
2511417306maltose+assimilation
2511437684mannose+assimilation
2511417309pectin+assimilation
2511415361pyruvate+assimilation
2511417992sucrose+assimilation
25114tryptone+assimilation

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment culture compositionenrichment culture durationenrichment culture temperature
24818wall of an active high-temperature white smoker chimney, 700 m depthNorwegian-Greenland Sea, Soria Moria vent field at the Mohns RidgeNorwayNOREurope71.2588-5.8122
25114wall of an active white smoker chimney (~270 C) located ~700 m below sea levelper litre: 3.03 g PIPES (Sigma), 20 g NaCl, 4.0 g Na2SO4 , 4.3 g MgCl2.6H2O, 0.25 g NH4Cl, 0.34 g KCl, 0.14 g CaCl2.2H2O, 0.14 g K2HPO4 .3H2O, 1.0 ml trace element solution, 0.002 g Fe(NH4)2(SO4)2 .6H2O and 0.001 g resazurin2 days55
67770Wall of an active hydrothermal white-smoker chimney in the Soria Moira vent field at the Mohns Ridge in the Norwegian-Greenland Sea

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Hydrothermal vent
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_86987.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_410;96_36163;97_45936;98_60162;99_86987&stattab=map
  • Last taxonomy: Marinitoga arctica subclade
  • 16S sequence: KX024844
  • Sequence Identity:
  • Total samples: 19
  • aquatic counts: 19

Safety information

risk assessment

  • @ref: 24818
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24818
  • description: Marinitoga arctica strain 2PyrY55-1 16S ribosomal RNA gene, partial sequence
  • accession: KX024844
  • length: 1494
  • database: ena
  • NCBI tax ID: 1862713

GC content

  • @ref: 24818
  • GC-content: 27
  • method: thermal denaturation, midpoint method (Tm)

External links

@ref: 24818

culture collection no.: DSM 29778, JCM 30566

straininfo link

  • @ref: 91300
  • straininfo: 408304

literature

  • topic: Phylogeny
  • Pubmed-ID: 27601246
  • title: Marinitoga arctica sp. nov., a thermophilic, anaerobic heterotroph isolated from a Mid-Ocean Ridge vent field.
  • authors: Steinsbu BO, Royseth V, Thorseth IH, Steen IH
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001472
  • year: 2016
  • mesh: Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, DNA-Directed DNA Polymerase, Fatty Acids/chemistry, Heterotrophic Processes, Hydrothermal Vents/*microbiology, Oceans and Seas, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24818Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-29778Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29778)
25114B. O. R. Steinsbu, V.,Thorseth, I. H.,Steen, I. H.Marinitoga arctica sp. nov., a thermophilic, anaerobic heterotroph isolated from a Mid-Ocean Ridge vent field10.1099/ijsem.0.001472IJSEM 66: 5070-5076 201627601246
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
91300Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID408304.1