Strain identifier
BacDive ID: 133030
Type strain:
Species: Marinitoga arctica
Strain Designation: 2PyrY55-1
Strain history: B. O. Steinsbu; Centre for Geobiol., Univ. of Bergen, Norway; 2PyrY55-1.
NCBI tax ID(s): 1862713 (species)
General
@ref: 24818
BacDive-ID: 133030
DSM-Number: 29778
keywords: 16S sequence, Bacteria, anaerobe, heterotroph, thermophilic, Gram-negative, motile, rod-shaped
description: Marinitoga arctica 2PyrY55-1 is an anaerobe, heterotroph, thermophilic bacterium that was isolated from wall of an active high-temperature white smoker chimney, 700 m depth.
NCBI tax id
- NCBI tax id: 1862713
- Matching level: species
strain history
@ref | history |
---|---|
24818 | <- B. O. Steinsbu, Univ. Bergen, Norway; 2PyrY55-1 |
67770 | B. O. Steinsbu; Centre for Geobiol., Univ. of Bergen, Norway; 2PyrY55-1. |
doi: 10.13145/bacdive133030.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/thermotogota
- domain: Bacteria
- phylum: Thermotogota
- class: Thermotogae
- order: Petrotogales
- family: Petrotogaceae
- genus: Marinitoga
- species: Marinitoga arctica
- full scientific name: Marinitoga arctica Steinsbu et al. 2016
@ref: 24818
domain: Bacteria
phylum: Thermotogae
class: Thermotogae
order: Thermotogales
family: Petrotogaceae
genus: Marinitoga
species: Marinitoga arctica
full scientific name: Marinitoga arctica Steinsbu et al. 2016
strain designation: 2PyrY55-1
type strain: yes
Morphology
cell morphology
- @ref: 25114
- gram stain: negative
- cell length: 1.0-5.0 µm
- cell width: 0.5-0.8 µm
- cell shape: rod-shaped
- motility: yes
- flagellum arrangement: monotrichous, polar
Culture and growth conditions
culture medium
- @ref: 24818
- name: MARINITOGA HYDROGENITOLERANS MEDIUM (DSMZ Medium 904a)
- growth: yes
- link: https://mediadive.dsmz.de/medium/904a
- composition: Name: MARINITOGA HYDROGENITOLERANS MEDIUM (DSMZ Medium 904a) Composition: Sea Salt 30.0 g/l MES 4.0 g/l D-Glucose 2.5 g/l Tryptone 1.0 g/l Yeast extract 1.0 g/l Na2S x 9 H2O 0.5 g/l L-Cysteine HCl x H2O 0.5 g/l Sodium resazurin 0.0005 g/l Distilled water
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
25114 | positive | growth | 45.0-70.0 | thermophilic |
25114 | positive | optimum | 65 | thermophilic |
24818 | positive | growth | 65 | thermophilic |
67770 | positive | growth | 65 | thermophilic |
culture pH
@ref | ability | type | pH |
---|---|---|---|
25114 | positive | growth | 5.0-7.5 |
25114 | positive | optimum | 5.5 |
Physiology and metabolism
oxygen tolerance
- @ref: 25114
- oxygen tolerance: anaerobe
nutrition type
- @ref: 25114
- type: heterotroph
halophily
@ref | salt | tested relation | concentration | growth |
---|---|---|---|---|
25114 | NaCl | optimum | 2.5 % | |
25114 | NaCl | growth | 1.5-5.5 %(w/v) | positive |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
25114 | 30089 | acetate | - | assimilation |
25114 | 85146 | carboxymethylcellulose | - | assimilation |
25114 | casamino acids | - | assimilation | |
25114 | casein | - | assimilation | |
25114 | 17029 | chitin | - | assimilation |
25114 | 28260 | galactose | - | assimilation |
25114 | 17716 | lactose | - | assimilation |
25114 | 33942 | ribose | - | assimilation |
25114 | 17057 | cellobiose | + | assimilation |
25114 | 62968 | cellulose | + | assimilation |
25114 | 15356 | cysteine | + | electron acceptor |
25114 | 33403 | elemental sulfur | + | electron acceptor |
25114 | 28757 | fructose | + | assimilation |
25114 | 17306 | maltose | + | assimilation |
25114 | 37684 | mannose | + | assimilation |
25114 | 17309 | pectin | + | assimilation |
25114 | 15361 | pyruvate | + | assimilation |
25114 | 17992 | sucrose | + | assimilation |
25114 | tryptone | + | assimilation |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | latitude | longitude | enrichment culture composition | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|---|---|
24818 | wall of an active high-temperature white smoker chimney, 700 m depth | Norwegian-Greenland Sea, Soria Moria vent field at the Mohns Ridge | Norway | NOR | Europe | 71.2588 | -5.8122 | |||
25114 | wall of an active white smoker chimney (~270 C) located ~700 m below sea level | per litre: 3.03 g PIPES (Sigma), 20 g NaCl, 4.0 g Na2SO4 , 4.3 g MgCl2.6H2O, 0.25 g NH4Cl, 0.34 g KCl, 0.14 g CaCl2.2H2O, 0.14 g K2HPO4 .3H2O, 1.0 ml trace element solution, 0.002 g Fe(NH4)2(SO4)2 .6H2O and 0.001 g resazurin | 2 days | 55 | ||||||
67770 | Wall of an active hydrothermal white-smoker chimney in the Soria Moira vent field at the Mohns Ridge in the Norwegian-Greenland Sea |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Hydrothermal vent |
#Condition | #Thermophilic (>45°C) |
taxonmaps
- @ref: 69479
- File name: preview.99_86987.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_410;96_36163;97_45936;98_60162;99_86987&stattab=map
- Last taxonomy: Marinitoga arctica subclade
- 16S sequence: KX024844
- Sequence Identity:
- Total samples: 19
- aquatic counts: 19
Safety information
risk assessment
- @ref: 24818
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24818
- description: Marinitoga arctica strain 2PyrY55-1 16S ribosomal RNA gene, partial sequence
- accession: KX024844
- length: 1494
- database: ena
- NCBI tax ID: 1862713
GC content
- @ref: 24818
- GC-content: 27
- method: thermal denaturation, midpoint method (Tm)
External links
@ref: 24818
culture collection no.: DSM 29778, JCM 30566
straininfo link
- @ref: 91300
- straininfo: 408304
literature
- topic: Phylogeny
- Pubmed-ID: 27601246
- title: Marinitoga arctica sp. nov., a thermophilic, anaerobic heterotroph isolated from a Mid-Ocean Ridge vent field.
- authors: Steinsbu BO, Royseth V, Thorseth IH, Steen IH
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001472
- year: 2016
- mesh: Bacteria, Anaerobic/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, DNA-Directed DNA Polymerase, Fatty Acids/chemistry, Heterotrophic Processes, Hydrothermal Vents/*microbiology, Oceans and Seas, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
24818 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29778 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29778) | |||
25114 | B. O. R. Steinsbu, V.,Thorseth, I. H.,Steen, I. H. | Marinitoga arctica sp. nov., a thermophilic, anaerobic heterotroph isolated from a Mid-Ocean Ridge vent field | 10.1099/ijsem.0.001472 | IJSEM 66: 5070-5076 2016 | 27601246 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
91300 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID408304.1 |