Strain identifier

BacDive ID: 133011

Type strain: Yes

Species: Scopulibacillus daqui

Strain Designation: ZQ18-1

Strain history: <- Y. Ge and S. Yao, China Natl. Res. Inst. of Food and Fermentation Ind., Beijing, China <- M. Zhang, CICC; ZQ18-1

NCBI tax ID(s): 1469162 (species)

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General

@ref: 24799

BacDive-ID: 133011

DSM-Number: 28236

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, spore-forming, Gram-positive, rod-shaped, colony-forming

description: Scopulibacillus daqui ZQ18-1 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms irregular colonies and was isolated from high temperature daqu of sesame-flavour liquor.

NCBI tax id

  • NCBI tax id: 1469162
  • Matching level: species

strain history

  • @ref: 24799
  • history: <- Y. Ge and S. Yao, China Natl. Res. Inst. of Food and Fermentation Ind., Beijing, China <- M. Zhang, CICC; ZQ18-1

doi: 10.13145/bacdive133011.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Sporolactobacillaceae
  • genus: Scopulibacillus
  • species: Scopulibacillus daqui
  • full scientific name: Scopulibacillus daqui Yao et al. 2016

@ref: 24799

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Sporolactobacillaceae

genus: Scopulibacillus

species: Scopulibacillus daqui

full scientific name: Scopulibacillus daqui Yao et al. 2016

strain designation: ZQ18-1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
25042positive1.4-2.3 µm0.7 µmrod-shapedno
69480positive100

colony morphology

  • @ref: 25042
  • colony color: white
  • colony shape: irregular
  • incubation period: 1 day
  • medium used: R2A agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25042Reasoner's 2A agar (R2A)yes
24799R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
25042positivegrowth20-60
25042positiveoptimum50thermophilic
24799positivegrowth55thermophilic

culture pH

@refabilitytypepHPH range
25042positivegrowth4.0-9.0alkaliphile
25042positiveoptimum8

Physiology and metabolism

oxygen tolerance

  • @ref: 25042
  • oxygen tolerance: facultative anaerobe

spore formation

@refspore descriptiontype of sporespore formationconfidence
25042oval with swollen sporangiaendosporeyes
69481yes97
69480yes99.998

halophily

@refsaltgrowthtested relationconcentration
25042NaClpositivegrowth0-10.0 %
25042NaCloptimum2 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
25042174265-dehydro-D-gluconate-builds acid from
2504285146carboxymethylcellulose-hydrolysis
25042casein-hydrolysis
2504217029chitin-hydrolysis
2504212936D-galactose-builds acid from
2504265327D-xylose-builds acid from
2504224265gluconate-builds acid from
2504217368hypoxanthine-hydrolysis
2504215443inulin-builds acid from
2504262345L-rhamnose-builds acid from
2504217716lactose-builds acid from
2504217306maltose-builds acid from
2504229864mannitol-builds acid from
25042320061methyl alpha-D-glucopyranoside-builds acid from
2504243943methyl alpha-D-mannoside-builds acid from
2504216634raffinose-builds acid from
2504230911sorbitol-builds acid from
2504228017starch-hydrolysis
2504217992sucrose-builds acid from
2504227897tryptophan-energy source
2504232528turanose-builds acid from
2504218186tyrosine-hydrolysis
2504215318xanthine-hydrolysis
2504216947citrate+carbon source
2504215824D-fructose+builds acid from
2504217634D-glucose+builds acid from
2504216024D-mannose+builds acid from
2504216988D-ribose+builds acid from
2504216443D-tagatose+builds acid from
250424853esculin+builds acid from
2504217754glycerol+builds acid from
2504230849L-arabinose+builds acid from
2504217266L-sorbose+builds acid from
25042506227N-acetylglucosamine+builds acid from
2504217632nitrate+reduction
2504227082trehalose+builds acid from

metabolite production

@refChebi-IDmetaboliteproduction
2504215688acetoinno
2504235581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
2504215688acetoin-
2504235581indole-

enzymes

@refvalueactivityec
25042arginine dihydrolase-3.5.3.6
25042catalase+1.11.1.6
25042cytochrome oxidase-1.9.3.1
25042lecithinase-
25042lysine decarboxylase-4.1.1.18
25042ornithine decarboxylase-4.1.1.17
25042tryptophan deaminase-4.1.99.1
25042tryptophan decarboxylase-4.1.1.27
25042urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture composition
24799high temperature daqu (saccharifying and fermenting agent) of sesame-flavour liquorShandong province, Bandaojing Co. Ltd.ChinaCHNAsia
25042R2A agar medium0.025% tryptone, 0.05% casamino acids, 0.05% soluble starch, 0.03% KH2PO4, 0.01% MgSO4, 0.03% sodium pyruvate, 0.025% pep- tone, 0.05% glucose and 2% agar (pH 7.2)

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Fermented
#Condition#Thermophilic (>45°C)
#Engineered#Food production#Beverage

taxonmaps

  • @ref: 69479
  • File name: preview.99_42782.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_414;96_8908;97_24560;98_31078;99_42782&stattab=map
  • Last taxonomy: Scopulibacillus daqui subclade
  • 16S sequence: KJ136650
  • Sequence Identity:
  • Total samples: 284
  • soil counts: 122
  • aquatic counts: 54
  • animal counts: 77
  • plant counts: 31

Safety information

risk assessment

  • @ref: 24799
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24799
  • description: Bacterium ZQ18-1 16S ribosomal RNA gene, partial sequence
  • accession: KJ136650
  • length: 1555
  • database: ena
  • NCBI tax ID: 1469162

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Scopulibacillus daqui DSM 28236GCA_016908855contigncbi1469162
66792Scopulibacillus daqui strain DSM 282361469162.3wgspatric1469162
66792Scopulibacillus daqui DSM 282362901206664draftimg1469162

GC content

  • @ref: 24799
  • GC-content: 40.5
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes97no
motileyes76.014yes
gram-positiveyes88.031no
anaerobicno98.929no
aerobicyes73.516yes
halophileyes59.167no
spore-formingyes93.06no
thermophileno83.306no
glucose-utilyes90.052no
flagellatedno58.006yes
glucose-fermentno87.49no

External links

@ref: 24799

culture collection no.: DSM 28236, CICC 10824

straininfo link

  • @ref: 91285
  • straininfo: 399529

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27515245Scopulibacillus daqui sp. nov., a thermophilic bacterium isolated from high temperature daqu.Yao S, Zhai L, Xin C, Liu Y, Xu L, Zhang X, Zhao T, Zhang L, Cheng CInt J Syst Evol Microbiol10.1099/ijsem.0.0014172016Bacillales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, *Food Microbiology, Hot Temperature, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Spores, Bacterial, Vitamin K 2/chemistryBiotechnology
Phylogeny29789976Scopulibacillus cellulosilyticus sp. nov., a cellulose-degrading bacterium isolated from tea.Yan ZF, Lin P, Li CT, Kook M, Yi THAntonie Van Leeuwenhoek10.1007/s10482-018-1101-12018Bacteria/classification/genetics/*metabolism, Cellulose/*metabolism, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Tea/*microbiologyMetabolism

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24799Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28236Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28236)
25042S. Z. Yao, L.,Xin, C.,Liu, Y.,Xu, L.,Zhang, X.,Zhao, T.,Zhang, L.,Cheng, C.Scopulibacillus daqui sp. nov., a thermophilic bacterium isolated from high temperature daqu10.1099/ijsem.0.001417IJSEM 66: 4723-4728 201627515245
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
91285Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399529.1