Strain identifier

BacDive ID: 133003

Type strain: Yes

Species: Ancylomarina subtilis

Strain Designation: FA102

Strain history: <- Zong-Jun Du, Shandong Univ., China

NCBI tax ID(s): 1639035 (species)

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General

@ref: 24791

BacDive-ID: 133003

DSM-Number: 28825

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, moderately halophilic, Gram-negative, filament-shaped, colony-forming

description: Ancylomarina subtilis FA102 is a facultative anaerobe, moderately halophilic, Gram-negative bacterium that forms circular colonies and was isolated from marine sediment.

NCBI tax id

  • NCBI tax id: 1639035
  • Matching level: species

strain history

@refhistory
24791<- Z.-J. Du, Shandong University at Weihai; FA102 <- Q. Liu
67771<- Zong-Jun Du, Shandong Univ., China

doi: 10.13145/bacdive133003.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Bacteroidia
  • order: Bacteroidales
  • family: Marinifilaceae
  • genus: Ancylomarina
  • species: Ancylomarina subtilis
  • full scientific name: Ancylomarina subtilis Wu et al. 2016

@ref: 24791

domain: Bacteria

phylum: Bacteroidetes

class: Bacteroidia

order: Bacteroidales

family: Marinifilaceae

genus: Ancylomarina

species: Ancylomarina subtilis

full scientific name: Ancylomarina subtilis Wu et al. 2016

strain designation: FA102

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
24917negative2.9-30.0 µm0.3-0.4 µmfilament-shapedno
67771filament-shapedno
67771negative
69480negative100

colony morphology

  • @ref: 24917
  • colony size: 1.0-1.5 mm
  • colony color: beige
  • colony shape: circular
  • incubation period: 2-3 days
  • medium used: 2216E agar (Hopebio)

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
249172216E agar (Hopebio)yes
24791BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
24917positivegrowth8.0-33.0
24917positiveoptimum28.0-30.0mesophilic
24791positivegrowth28mesophilic
67771positivegrowth28-30mesophilic

culture pH

@refabilitytypepHPH range
24917positivegrowth6.0-8.5alkaliphile
24917positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
24917facultative anaerobe
67771facultative anaerobe

spore formation

@refspore formationconfidence
24917no
69481no100
69480no99.998

halophily

@refhalophily levelsaltgrowthtested relationconcentration
24917moderately halophilicNaClpositivegrowth0.5-5.0 %(w/v)
24917NaCloptimum2 %(w/v)

observation

  • @ref: 67771
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2491785146carboxymethylcellulose-hydrolysis
2491717057cellobiose-builds acid from
2491716947citrate-carbon source
249174853esculin-builds acid from
2491717632nitrate-reduction
2491728017starch-hydrolysis
2491753426tween 80-hydrolysis
2491715589L-malate+oxidation
24917174265-dehydro-D-gluconate+builds acid from
249172509agar+hydrolysis
2491758187alginate+hydrolysis
2491712936D-galactose+builds acid from
2491717634D-glucose+builds acid from
249174853esculin+hydrolysis
249175291gelatin+hydrolysis
2491732323glucuronamide+oxidation
24917506227N-acetylglucosamine+builds acid from
24917O-nitrophenyl-beta-D-galactopyranosid+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
2491715688acetoinno
2491716136hydrogen sulfideno
2491735581indoleyes

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testcitrate test
2491715688acetoin-
2491716947citrate-

enzymes

@refvalueactivityec
24917acid phosphatase+3.1.3.2
24917alkaline phosphatase+3.1.3.1
24917alpha-galactosidase-3.2.1.22
24917alpha-glucosidase-3.2.1.20
24917alpha-mannosidase-3.2.1.24
24917arginine dihydrolase-3.5.3.6
24917beta-D-fucosidase-3.2.1.38
24917beta-galactosidase-3.2.1.23
24917beta-glucosidase-3.2.1.21
24917beta-glucuronidase+3.2.1.31
24917beta-glucuronidase-3.2.1.31
24917catalase-1.11.1.6
24917chymotrypsin+3.4.4.5
24917cystine arylamidase-3.4.11.3
24917cytochrome oxidase-1.9.3.1
24917esterase (C 4)+
24917esterase lipase (C 8)-
24917gelatinase+
24917leucine arylamidase-3.4.11.1
24917lipase (C 14)-
24917lysine decarboxylase-4.1.1.18
24917N-acetyl-beta-glucosaminidase+3.2.1.52
24917naphthol-AS-BI-phosphohydrolase+
24917ornithine decarboxylase-4.1.1.17
24917trypsin-3.4.21.4
24917tryptophan deaminase-4.1.99.1
24917urease-3.5.1.5
24917valine arylamidase-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment culture durationenrichment culture temperature
24791marine sedimentcoast of Weihai (37° 32' 01.93'' N 122° 03' 44.01'' E)ChinaCHNAsia37.5339122.062
249175 days25
67771From marine sedimentcoaste of WeihaiChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Aquatic#Sediment

taxonmaps

  • @ref: 69479
  • File name: preview.99_156398.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15786;96_44584;97_57098;98_103104;99_156398&stattab=map
  • Last taxonomy: Ancylomarina subtilis subclade
  • 16S sequence: KP214056
  • Sequence Identity:
  • Total samples: 261
  • soil counts: 18
  • aquatic counts: 226
  • animal counts: 7
  • plant counts: 10

Safety information

risk assessment

  • @ref: 24791
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24791
  • description: Ancylomarina subtilis strain FA102 16S ribosomal RNA gene, partial sequence
  • accession: KP214056
  • length: 1450
  • database: ena
  • NCBI tax ID: 1639035

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Ancylomarina subtilis DSM 28825GCA_004217115scaffoldncbi1639035
66792Ancylomarina subtilis strain DSM 288251639035.3wgspatric1639035
66792Ancylomarina subtilis DSM 288252795386138draftimg1639035

GC content

@refGC-contentmethod
2479136.5high performance liquid chromatography (HPLC)
6777136.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno90.368no
gram-positiveno97.972yes
anaerobicno54.632yes
aerobicno95.326yes
halophileyes66.663yes
spore-formingno92.707yes
glucose-utilyes80.448no
thermophileno99.26no
motileno88.658yes
glucose-fermentno58.881no

External links

@ref: 24791

culture collection no.: DSM 28825, CICC 10902, KCTC 42257

straininfo link

  • @ref: 91279
  • straininfo: 399341

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27470589Description of Ancylomarina subtilis gen. nov., sp. nov., isolated from coastal sediment, proposal of Marinilabiliales ord. nov. and transfer of Marinilabiliaceae, Prolixibacteraceae and Marinifilaceae to the order Marinilabiliales.Wu WJ, Zhao JX, Chen GJ, Du ZJInt J Syst Evol Microbiol10.1099/ijsem.0.0013422016Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Geologic Sediments/*microbiology, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny29453612Ancylomarina psychrotolerans sp. nov., isolated from sediments of Fildes Peninsula and emended the description of genus Ancylomarina.Jia C, Cui HC, Han YQ, Fu TY, Du R, Wang XL, Shi XC, Zhang XHAntonie Van Leeuwenhoek10.1007/s10482-018-1025-92018Antarctic Regions, Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/*isolation & purification/metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Geologic Sediments/*microbiology, Phospholipids/metabolism, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24791Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28825Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28825)
24917W.-J. Z. Wu, Jin-Xin,Chen, Guan-Jun,Du, Zong-JunDescription of Ancylomarina subtilis gen. nov., sp. nov., isolated from coastal sediment, proposal of Marinilabiliales ord. nov. and transfer of Marinilabiliaceae, Prolixibacteraceae and Marinifilaceae to the order Marinilabiliales10.1099/ijsem.0.001342IJSEM 66: 4243-4249 201627470589
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
91279Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399341.1