Strain identifier
BacDive ID: 132990
Type strain: ![]()
Species: Paraburkholderia metalliresistens
Strain Designation: D414
Strain history: <- J. Guo, Centre for Res. in Ecotoxicology and Environmental Remediation, Agro-Environmental Protection Inst., Ministry of Agriculture, Tianjin, China; D414
NCBI tax ID(s): 1398664 (species)
General
@ref: 24778
BacDive-ID: 132990
DSM-Number: 26823
keywords: 16S sequence, Bacteria, mesophilic
description: Paraburkholderia metalliresistens D414 is a mesophilic bacterium that was isolated from arable layer of a heavy metal-polluted paddy soil, mine.
NCBI tax id
- NCBI tax id: 1398664
- Matching level: species
strain history
- @ref: 24778
- history: <- J. Guo, Centre for Res. in Ecotoxicology and Environmental Remediation, Agro-Environmental Protection Inst., Ministry of Agriculture, Tianjin, China; D414
doi: 10.13145/bacdive132990.20241212.9.2
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Burkholderiales
- family: Burkholderiaceae
- genus: Paraburkholderia
- species: Paraburkholderia metalliresistens
- full scientific name: Paraburkholderia metalliresistens (Guo et al. 2015) Dobritsa and Samadpour 2016
synonyms
- @ref: 20215
- synonym: Burkholderia metalliresistens
@ref: 24778
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Burkholderiales
family: Burkholderiaceae
genus: Paraburkholderia
species: Paraburkholderia metalliresistens
full scientific name: Paraburkholderia metalliresistens (Guo et al. 2015) Dobritsa and Samadpour 2016
strain designation: D414
type strain: yes
Culture and growth conditions
culture medium
- @ref: 24778
- name: R2A MEDIUM (DSMZ Medium 830)
- growth: yes
- link: https://mediadive.dsmz.de/medium/830
- composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
culture temp
- @ref: 24778
- growth: positive
- type: growth
- temperature: 30
Physiology and metabolism
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 27689 | decanoate | + | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | + | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | + | assimilation |
| 68369 | 30849 | L-arabinose | + | assimilation |
| 68369 | 17634 | D-glucose | + | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 4853 | esculin | + | hydrolysis |
| 68369 | 16199 | urea | - | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
| 68369 | 17632 | nitrate | - | reduction |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68369 | urease | - | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
| 68369 | cytochrome oxidase | + | 1.9.3.1 |
| 68369 | gelatinase | - | |
| 68369 | beta-glucosidase | + | 3.2.1.21 |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC | OX |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 24778 | - | - | - | - | - | + | - | + | + | + | + | + | + | - | + | + | +/- | + | + | + | + |
| 24778 | - | - | - | - | - | + | - | + | + | + | + | + | + | - | + | + | +/- | + | + | + |
Isolation, sampling and environmental information
isolation
- @ref: 24778
- sample type: arable layer (0–20 cm) of a heavy metal-polluted paddy soil, mine
- geographic location: Guangdong Province, Dabao Mountain Mine
- country: China
- origin.country: CHN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Agriculture | #Field |
| #Engineered | #Contamination | #Heavy metal |
| #Engineered | #Other | #Mine |
| #Environmental | #Terrestrial | #Soil |
taxonmaps
- @ref: 69479
- File name: preview.99_18677.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_12;96_624;97_727;98_8909;99_18677&stattab=map
- Last taxonomy: Paraburkholderia
- 16S sequence: KF601211
- Sequence Identity:
- Total samples: 895
- soil counts: 513
- aquatic counts: 45
- animal counts: 195
- plant counts: 142
Safety information
risk assessment
- @ref: 24778
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24778
- description: Paraburkholderia metalliresistens strain D414 16S ribosomal RNA gene, partial sequence
- accession: KF601211
- length: 1437
- database: nuccore
- NCBI tax ID: 1398664
GC content
- @ref: 24778
- GC-content: 62.3
- method: high performance liquid chromatography (HPLC)
External links
@ref: 24778
culture collection no.: DSM 26823, CICC 10561
straininfo link
- @ref: 91266
- straininfo: 403410
literature
- topic: Phylogeny
- Pubmed-ID: 25896306
- title: Burkholderia metalliresistens sp. nov., a multiple metal-resistant and phosphate-solubilising species isolated from heavy metal-polluted soil in Southeast China.
- authors: Guo JK, Ding YZ, Feng RW, Wang RG, Xu YM, Chen C, Wei XL, Chen WM
- journal: Antonie Van Leeuwenhoek
- DOI: 10.1007/s10482-015-0453-z
- year: 2015
- mesh: Bacterial Typing Techniques, Base Composition, Burkholderia/*classification/genetics/*isolation & purification/physiology, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, *Drug Resistance, Bacterial, Fatty Acids/analysis, Indoles/metabolism, Metals, Heavy/*toxicity, Microbial Sensitivity Tests, Microscopy, Electron, Scanning, Molecular Sequence Data, Nucleic Acid Hybridization, Phosphates/*metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants/toxicity
- topic2: Metabolism
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 24778 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26823 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26823) | |
| 68369 | Automatically annotated from API 20NE | |||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |
| 91266 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID403410.1 |