Strain identifier

BacDive ID: 132989

Type strain: Yes

Species: Chenggangzhangella methanolivorans

Strain history: <- L. Yang, CAS; CHL1

NCBI tax ID(s): 1437009 (species)

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General

@ref: 24777

BacDive-ID: 132989

DSM-Number: 100641

keywords: 16S sequence, Bacteria, aerobe, Gram-negative, rod-shaped, colony-forming

description: Chenggangzhangella methanolivorans DSM 100641 is an aerobe, Gram-negative, rod-shaped bacterium that forms circular colonies and was isolated from sludge from a sewage treatment tank at an agricultural chemical factory.

NCBI tax id

  • NCBI tax id: 1437009
  • Matching level: species

strain history

  • @ref: 24777
  • history: <- L. Yang, CAS; CHL1

doi: 10.13145/bacdive132989.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Methylocystaceae
  • genus: Chenggangzhangella
  • species: Chenggangzhangella methanolivorans
  • full scientific name: Chenggangzhangella methanolivorans Yang et al. 2016

@ref: 24777

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Methylocystaceae

genus: Chenggangzhangella

species: Chenggangzhangella methanolivorans

full scientific name: Chenggangzhangella methanolivorans Yang et al. 2016

type strain: yes

Morphology

cell morphology

  • @ref: 43584
  • gram stain: negative
  • cell length: 1.2-1.8 µm
  • cell width: 0.9-1 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 43584
  • colony size: 1-2 mm
  • colony color: White
  • colony shape: circular
  • incubation period: 3 days
  • medium used: PBMM agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43584PBMM agaryes
24777R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperature
43584positivegrowth5-40
43584positiveoptimum28-30
24777positivegrowth28

culture pH

@refabilitytypepH
43584positivegrowth6-8
43584positiveoptimum7

Physiology and metabolism

oxygen tolerance

  • @ref: 43584
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43584
  • type of spore: endospore
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
43584NaClpositivegrowth0.5-5 %(w/v)
43584NaClpositiveoptimum2.5 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4358417634D-glucose-assimilation
4358416899D-mannitol-assimilation
4358416024D-mannose-assimilation
43584506227N-acetylglucosamine-assimilation
4358418401phenylacetate-assimilation
4358432032potassium gluconate-assimilation
4358453258sodium citrate-assimilation
4358416651(S)-lactate-carbon source
43584167632-oxobutanoate-carbon source
4358413705acetoacetate-carbon source
4358417925alpha-D-glucose-carbon source
4358436219alpha-lactose-carbon source
43584645522-hydroxybutyrate-carbon source
4358417057cellobiose-carbon source
4358416947citrate-carbon source
4358418333D-arabitol-carbon source
4358415824D-fructose-carbon source
4358428847D-fucose-carbon source
4358412936D-galactose-carbon source
4358416899D-mannitol-carbon source
4358416024D-mannose-carbon source
4358416634raffinose-carbon source
4358417924D-sorbitol-carbon source
4358423652dextrin-carbon source
4358416865gamma-aminobutyric acid-carbon source
435845291gelatin-carbon source
4358428066gentiobiose-carbon source
4358417754glycerol-carbon source
4358417596inosine-carbon source
4358418287L-fucose-carbon source
4358462345L-rhamnose-carbon source
4358417306maltose-carbon source
4358428053melibiose-carbon source
4358417309pectin-carbon source
4358417272propionate-carbon source
4358417164stachyose-carbon source
4358417992sucrose-carbon source
4358427082trehalose-carbon source
4358432528turanose-carbon source
435844853esculin-hydrolysis
435845291gelatin-hydrolysis
4358453424tween 20-hydrolysis
4358453423tween 40-hydrolysis
4358417632nitrate-reduction
4358417128adipate+assimilation
4358427689decanoate+assimilation
4358430849L-arabinose+assimilation
4358425115malate+assimilation
4358417306maltose+assimilation
4358417634D-glucose+builds acid from
4358415588D-malate+carbon source
4358430089acetate+carbon source
4358473706bromosuccinate+carbon source
435848391D-gluconate+carbon source
4358433801D-saccharate+carbon source
4358415740formate+carbon source
4358432323glucuronamide+carbon source
4358429991L-aspartate+carbon source
4358429985L-glutamate+carbon source
4358451850methyl pyruvate+carbon source
4358417268myo-inositol+carbon source
4358453425tween 60+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
4358416136hydrogen sulfideno
4358435581indoleno

enzymes

@refvalueactivityec
43584cytochrome oxidase+1.9.3.1
43584catalase+1.11.1.6
43584alkaline phosphatase+3.1.3.1
43584esterase (C 4)+
43584esterase Lipase (C 8)+
43584lipase (C 14)+
43584leucine arylamidase+3.4.11.1
43584valine arylamidase+
43584cystine arylamidase+3.4.11.3
43584trypsin+3.4.21.4
43584acid phosphatase+3.1.3.2
43584naphthol-AS-BI-phosphohydrolase+
43584amylase-
43584cellulase-3.2.1.4
43584alpha-chymotrypsin-3.4.21.1
43584alpha-galactosidase-3.2.1.22
43584beta-galactosidase-3.2.1.23
43584beta-glucuronidase-3.2.1.31
43584alpha-glucosidase-3.2.1.20
43584beta-glucosidase-3.2.1.21
43584N-acetyl-beta-glucosaminidase-3.2.1.52
43584alpha-mannosidase-3.2.1.24
43584beta-D-fucosidase-3.2.1.38
43584arginine dihydrolase-3.5.3.6
43584urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
24777sludge from a sewage treatment tank at an agricultural chemical factoryJiangsu ProvinceChinaCHNAsia
43584a sludge sample collected from a sewage treatment tank at an agricultural chemical factoryJiangsu ProvicneChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture
#Engineered#Waste#Wastewater
#Environmental#Terrestrial#Mud (Sludge)
#Engineered#Built environment#Container (Reservoir)
#Engineered#Industrial#Plant (Factory)
#Engineered#Waste#Sewage sludge

taxonmaps

  • @ref: 69479
  • File name: preview.99_139998.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_1101;97_1289;98_93077;99_139998&stattab=map
  • Last taxonomy: Chenggangzhangella methanolivorans subclade
  • 16S sequence: KF726142
  • Sequence Identity:
  • Total samples: 105
  • soil counts: 10
  • aquatic counts: 37
  • animal counts: 45
  • plant counts: 13

Safety information

risk assessment

  • @ref: 24777
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24777
  • description: Chenggangzhangella methanolivorans strain CHL1 16S ribosomal RNA gene, partial sequence
  • accession: KF726142
  • length: 1448
  • database: nuccore
  • NCBI tax ID: 1437009

GC content

  • @ref: 24777
  • GC-content: 70.4
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24777

culture collection no.: DSM 100641, CCTCC AB 2015175, KCTC 42661, CHL 1

straininfo link

  • @ref: 91265
  • straininfo: 400585

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny27046027Chenggangzhangella methanolivorans gen. nov., sp. nov., a member of the family Methylocystaceae, transfer of Methylopila helvetica Doronina et al. 2000 to Albibacter helveticus comb. nov. and emended description of the genus Albibacter.Yang LQ, Liu L, Salam N, Xiao M, Kim CJ, Hozzein WN, Park DJ, Li WJ, Zhang HWInt J Syst Evol Microbiol10.1099/ijsem.0.0010622016Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, Methylocystaceae/*classification/genetics/isolation & purification, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Ubiquinone/analogs & derivatives/analysisTranscriptome
Metabolism29621247Dynamic changes in microbial communities during the bioremediation of herbicide (chlorimuron-ethyl and atrazine) contaminated soils by combined degrading bacteria.Wang J, Li X, Li X, Wang H, Su Z, Wang X, Zhang HPLoS One10.1371/journal.pone.01947532018Analysis of Variance, Atrazine/*metabolism, Bacteria/*metabolism, *Biodegradation, Environmental, Herbicides/*metabolism, *Microbiota, Pyrimidines/*metabolism, Soil Pollutants/*metabolism, Sulfonylurea Compounds/*metabolism, Time Factors
32951166High bioremediation potential of strain Chenggangzhangella methanolivorans CHL1 for soil polluted with metsulfuron-methyl or tribenuron-methyl in a pot experiment.Yang TT, Zhang HW, Wang J, Li XY, Li X, Su ZCEnviron Sci Pollut Res Int10.1007/s11356-020-10825-w2020Arylsulfonates, Biodegradation, Environmental, *Herbicides, Methylocystaceae, Soil, Soil Microbiology, *Soil Pollutants/analysis
Metabolism35861510Whole-Genome Sequencing of a Chlorimuron-Ethyl-Degrading Strain: Chenggangzhangella methanolivorans CHL1 and Its Degrading Enzymes.Yu Z, Gu W, Yang Y, Li X, Li X, Li T, Wang J, Su Z, Li X, Dai Y, Xu M, Zhang HMicrobiol Spectr10.1128/spectrum.01822-222022Bacteria/metabolism, Escherichia coli/genetics/metabolism, Glutathione, *Herbicides/metabolism, Methylocystaceae, Pyrimidines, *Soil Pollutants/metabolism, Sulfonylurea Compounds, TransferasesEnzymology
Metabolism36077288A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms.Yu Z, Dai Y, Li T, Gu W, Yang Y, Li X, Peng P, Yang L, Li X, Wang J, Su Z, Li X, Xu M, Zhang HInt J Mol Sci10.3390/ijms231798902022Biodegradation, Environmental, *Herbicides/metabolism, *Methylocystaceae/metabolism, Pyrimidines/metabolism, *Soil Pollutants/metabolism, Sulfonylurea Compounds/metabolism

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24777Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-100641Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100641)
43584Li-Qiang Yang, Lan Liu, Nimaichand Salam, Min Xiao, Chang-Jin Kim, Wael N. Hozzein, Dong-Jin Park, Wen-Jun Li, Hui-Wen ZhangChenggangzhangella methanolivorans gen. nov., sp. nov., a member of the family Methylocystaceae, transfer of Methylopila helvetica Doronina et al. 2000 to Albibacter helveticus comb. nov. and emended description of the genus Albibacter10.1099/ijsem.0.001062IJSEM 66: 2825-2830 201627046027
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
91265Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID400585.1