Strain identifier

BacDive ID: 13298

Type strain: Yes

Species: Lentzea aerocolonigenes

Strain Designation: 701

Strain history: CIP <- 2001, DSMZ <- E.B. Shirling, Streptomyces aerocolonigenes <- R. Shinobu: strain 701, Nocardia sp.

NCBI tax ID(s): 68170 (species)

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General

@ref: 9233

BacDive-ID: 13298

DSM-Number: 40034

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, spore-forming, mesophilic, human pathogen

description: Lentzea aerocolonigenes 701 is an obligate aerobe, spore-forming, mesophilic human pathogen that was isolated from soil.

NCBI tax id

  • NCBI tax id: 68170
  • Matching level: species

strain history

@refhistory
9233<- E.B. Shirling, ISP (Streptomyces aerocolonigenes) <- R. Shinobu, 701
67770KCC S-0150 <-- R. Shinobu 701.
116063CIP <- 2001, DSMZ <- E.B. Shirling, Streptomyces aerocolonigenes <- R. Shinobu: strain 701, Nocardia sp.

doi: 10.13145/bacdive13298.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Lentzea
  • species: Lentzea aerocolonigenes
  • full scientific name: Lentzea aerocolonigenes (Labeda 1986 ex Shinobu and Kawato 1960) Nouioui et al. 2018

  • synonyms

    @refsynonym
    20215Saccharothrix aerocolonigenes
    20215Lechevalieria aerocolonigenes
    20215Nocardia aerocolonigenes
    20215Streptomyces aerocolonigenes

@ref: 9233

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Lentzea

species: Lentzea aerocolonigenes

full scientific name: Lentzea aerocolonigenes (Labeda 1986) Nouioui et al. 2018

strain designation: 701

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.286
69480100positive
116063nopositiverod-shaped

colony morphology

  • @ref: 116063
  • hemolysis ability: 1

multimedia

  • @ref: 9233
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_40034.jpg
  • caption: Medium 215 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
9233BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
40673MEDIUM 57 - for Streptomyces, Nocardioides, Lentzea albidocapillata and Streptoverticillium reticulumyesDistilled water make up to (1000.000 ml);Agar (15.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g);Calcium carbonate (2.000 g)
116063CIP Medium 57yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=57

culture temp

@refgrowthtypetemperaturerange
9233positivegrowth28mesophilic
18469positiveoptimum28mesophilic
40673positivegrowth30mesophilic
67770positivegrowth28mesophilic
116063positivegrowth25-41
116063nogrowth10psychrophilic
116063nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116063
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: yes
  • confidence: 100

compound production

@refcompound
9233restriction endonuclease NaeI
20216Endonuclease NAe I

halophily

@refsaltgrowthtested relationconcentration
18469NaClpositivemaximum2.5 %
116063NaClpositivegrowth0-6 %
116063NaClnogrowth8 %
116063NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371melibiose-builds acid from28053
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
68368gelatin+hydrolysis5291
68368tryptophan-energy source27897
68368urea+hydrolysis16199
68368citrate+assimilation16947
68368ornithine+degradation18257
68368lysine+degradation25094
68368arginine+hydrolysis29016
116063citrate-carbon source16947
116063esculin+hydrolysis4853
116063hippurate-hydrolysis606565
116063nitrate+reduction17632
116063nitrite-reduction16301
116063nitrate-respiration17632

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinyes
6836835581indoleno
6836816136hydrogen sulfideno
11606335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin+
6836835581indole-
11606315688acetoin-
11606317234glucose-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase+3.2.1.23
116063oxidase-
116063beta-galactosidase+3.2.1.23
116063alcohol dehydrogenase-1.1.1.1
116063gelatinase+
116063amylase-
116063DNase+
116063caseinase+3.4.21.50
116063catalase+1.11.1.6
116063tween esterase+
116063gamma-glutamyltransferase+2.3.2.2
116063lecithinase-
116063lipase-
116063lysine decarboxylase-4.1.1.18
116063ornithine decarboxylase-4.1.1.17
116063phenylalanine ammonia-lyase-4.3.1.24
116063protease+
116063tryptophan deaminase-
116063urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382lipase (C 14)-
68382cystine arylamidase+3.4.11.3
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382beta-glucuronidase-3.2.1.31
68382N-acetyl-beta-glucosaminidase+3.2.1.52

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
18469++----+++++-----+-+
116063+++-+++-+++++-++++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18469+++++-+--++

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
116063--+/-+/-+/------+/----+/------+/----+/---+/-+/--+/------+/---+/-+/---+/------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116063+++++-++-++++++++-++++-++-+----+++-+---+--------++++--+-+--++-+-+----+-+-+-+---++---+-+-++++-+-+++-

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic locationisolation date
9233soilJapanJPNAsia
67770SoilJapanJPNAsiaNagasaki City
116063Environment, SoilJapanJPNAsia1990

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_2659.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_840;97_982;98_2059;99_2659&stattab=map
  • Last taxonomy: Lentzea
  • 16S sequence: AB297960
  • Sequence Identity:
  • Total samples: 143
  • soil counts: 78
  • animal counts: 8
  • plant counts: 57

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
9233yes, in single cases1Risk group (German classification)
184691German classification
1160631Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lechevalieria aerocolonigenes 16S ribosomal RNA gene, partial sequenceAF3286811440ena68170
20218Lechevalieria aerocolonigenes gene for 16S rRNA, strain: ISP 5034AB0200301476ena68170
20218Lechevalieria aerocolonigenes gene for 16S ribosomal RNA, partial sequence, strain: JCM 4150D44017120ena68170
20218Lechevalieria aerocolonigenes gene for 16S rRNA, partial sequenceAB2979601442ena68170
20218Lechevalieria aerocolonigenes gene for 16S rRNA, partial sequence, strain: NBRC 13195AB3272561442ena68170
20218Lechevalieria aerocolonigenes strain NRRL B-3298T 16S ribosomal RNA gene, partial sequenceAF1148041509ena68170

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lentzea aerocolonigenes NBRC 13195GCA_000974445scaffoldncbi68170
66792Lechevalieria aerocolonigenes strain NBRC 1319568170.11wgspatric68170
66792Lentzea aerocolonigenes DSM 400342596583536draftimg68170
66792Lentzea aerocolonigenes NBRC 131952645727610draftimg68170
67770Lentzea aerocolonigenes NRRL B-3298GCA_000719205contigncbi68170
66792Lentzea aerocolonigenes DSM 40034GCA_024171805scaffoldncbi68170

GC content

@refGC-contentmethod
6777070thermal denaturation, midpoint method (Tm)
6777068.9genome sequence analysis
6777070-71thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes87no
motileno89.547no
flagellatedno96.146no
gram-positiveyes87.31no
anaerobicno98.948no
aerobicyes90.798no
halophileno91.3no
spore-formingyes93.941no
glucose-utilyes88.462no
thermophileno99.044no
glucose-fermentno91.213no

External links

@ref: 9233

culture collection no.: DSM 40034, ATCC 23870, CBS 609.68, CIP 107109, IFO 13195, IFO 3837, ISP 5034, NBRC 13195, NBRC 3837, NRRL B-3298, RIA 1108, Shinobu 701, JCM 4150, BCRC 13661, CCM 2777, CGMCC 4.1712, IMET 7515, IMSNU 21350, JCM 4614, KCTC 9379, MTCC 1533, NCIMB 12944, VKM Ac-1081

straininfo link

  • @ref: 82499
  • straininfo: 38277

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11411672Revival of the genus Lentzea and proposal for Lechevalieria gen. nov.Labeda DP, Hatano K, Kroppenstedt RM, Tamura TInt J Syst Evol Microbiol10.1099/00207713-51-3-10452001Actinomycetales/*classification/genetics/physiology, Carbohydrate Metabolism, Carboxylic Acids/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny28950934Lechevalieria rhizosphaerae sp. nov., a novel actinomycete isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Lechevalieria.Zhao J, Li W, Shi L, Wang H, Wang Y, Zhao Y, Xiang W, Wang XInt J Syst Evol Microbiol10.1099/ijsem.0.0023512017Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, *Rhizosphere, Sequence Analysis, DNA, *Soil Microbiology, Triticum/*microbiology, Vitamin K 2/analogs & derivatives/chemistryTranscriptome
Phylogeny32699968Lentzea indica sp. nov., a novel actinobacteria isolated from Indian Himalayan-soil.Maiti PK, Mandal SAntonie Van Leeuwenhoek10.1007/s10482-020-01449-82020Actinobacteria/*classification/growth & development/*isolation & purification, *Bacterial Typing Techniques, Genotyping Techniques, India, Mycelium, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil MicrobiologyPhenotype

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
9233Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 40034)https://www.dsmz.de/collection/catalogue/details/culture/DSM-40034
18469Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM40034.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40673Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4509
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
82499Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID38277.1StrainInfo: A central database for resolving microbial strain identifiers
116063Curators of the CIPCollection of Institut Pasteur (CIP 107109)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107109