Strain identifier

BacDive ID: 13297

Type strain: Yes

Species: Lentzea flava

Strain history: CIP <- 2001, DSMZ <- N.S. Agre, VKM, Nocardiopsis flava

NCBI tax ID(s): 103732 (species)

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General

@ref: 11364

BacDive-ID: 13297

DSM-Number: 43885

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-positive, rod-shaped, antibiotic compound production

description: Lentzea flava DSM 43885 is an obligate aerobe, mesophilic, Gram-positive bacterium that produces antibiotic compounds and was isolated from soil.

NCBI tax id

  • NCBI tax id: 103732
  • Matching level: species

strain history

@refhistory
11364<- N. S. Agre, VKM (Nocardiopsis flava)
67770KCC A-0296 <-- MS 2136 <-- ATCC 29533 <-- INA 2171.
119364CIP <- 2001, DSMZ <- N.S. Agre, VKM, Nocardiopsis flava

doi: 10.13145/bacdive13297.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Pseudonocardiales
  • family: Pseudonocardiaceae
  • genus: Lentzea
  • species: Lentzea flava
  • full scientific name: Lentzea flava (Gauze et al. 1974) Nouioui et al. 2018

  • synonyms

    @refsynonym
    20215Actinomadura flava
    20215Lechevalieria flava
    20215Nocardiopsis flava
    20215Saccharothrix flava

@ref: 11364

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Lentzea

species: Lentzea flava

full scientific name: Lentzea flava (Gauze et al. 1974) Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

  • @ref: 119364
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium usedhemolysis ability
19612Maize yellow10-14 daysISP 2
1961210-14 daysISP 3
1961210-14 daysISP 4
19612Chrome yellow10-14 daysISP 5
1961210-14 daysISP 6
19612Brown beige10-14 daysISP 7
1193641

multicellular morphology

@refforms multicellular complexmedium name
19612noISP 2
19612noISP 3
19612noISP 4
19612noISP 5
19612noISP 6
19612noISP 7

multimedia

  • @ref: 11364
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43885.jpg
  • caption: Medium 65 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11364ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)yeshttps://mediadive.dsmz.de/medium/84Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
11364GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19612ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19612ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19612ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19612ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19612ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19612ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
40462MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119364CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
19612positiveoptimum37mesophilic
11364positivegrowth37mesophilic
40462positivegrowth30mesophilic
67770positivegrowth28mesophilic
119364positivegrowth22-37
119364nogrowth10psychrophilic
119364nogrowth15psychrophilic
119364nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 119364
  • oxygen tolerance: obligate aerobe

compound production

@refcompound
11364madumycin
20216Madumycin
67770Madumycin

halophily

@refsaltgrowthtested relationconcentration
19612NaClpositivemaximum2.5 %
119364NaClpositivegrowth0-4 %
119364NaClnogrowth6 %
119364NaClnogrowth8 %
119364NaClnogrowth10 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1961217234glucose+
1961222599arabinose+
1961217992sucrose+
1961218222xylose-
1961217268myo-inositol+
1961229864mannitol+
1961228757fructose+
1961226546rhamnose+
1961216634raffinose-
1961262968cellulose-
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117306maltose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837117634D-glucose-builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea+hydrolysis
6836816947citrate+assimilation
6836818257ornithine+degradation
6836825094lysine+degradation
6836829016arginine-hydrolysis
11936416947citrate-carbon source
1193644853esculin+hydrolysis
119364606565hippurate-hydrolysis
11936417632nitrate+reduction
11936416301nitrite-reduction
11936417632nitrate-respiration

metabolite production

@refChebi-IDmetaboliteproduction
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11936435581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole testmethylred-test
6836815688acetoin-
6836835581indole-
11936415688acetoin-
11936417234glucose-

enzymes

@refvalueactivityec
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease+3.5.1.5
68368ornithine decarboxylase+4.1.1.17
68368lysine decarboxylase+4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase+3.2.1.23
119364oxidase-
119364beta-galactosidase-3.2.1.23
119364alcohol dehydrogenase-1.1.1.1
119364gelatinase+
119364amylase+
119364DNase+
119364catalase+1.11.1.6
119364tween esterase-
119364gamma-glutamyltransferase+2.3.2.2
119364lecithinase-
119364lipase-
119364lysine decarboxylase-4.1.1.18
119364ornithine decarboxylase-4.1.1.17
119364phenylalanine ammonia-lyase-4.3.1.24
119364protease+
119364tryptophan deaminase-
119364urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382alpha-chymotrypsin+3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase+3.2.1.22
68382beta-galactosidase+3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382alpha-fucosidase-3.2.1.51

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19612+-+-+++++++++-+-++-
119364+++-+++-+++++-+++---

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
19612+-+++-+---+

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119364--+/------------+/----------+/----+/---------------+/------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119364+++++-++-+++++-++++++++++-+----+++-+---+---------++--+-----++-+----------+-+---++-----+-++++-++-+-+

Isolation, sampling and environmental information

isolation

@refsample typeisolation date
11364soil
119364Environment, Soil1992

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
113641Risk group (German classification)
196121Risk group (German classification)
1193641Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Lechevalieria flava strain NRRL B-16131T 16S ribosomal RNA gene, partial sequence
  • accession: AF114808
  • length: 1510
  • database: ena
  • NCBI tax ID: 103732

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lentzea flava JCM 3296GCA_014648475scaffoldncbi103732
66792Lechevalieria flava strain JCM 3296103732.3wgspatric103732
66792Lentzea flava DSM 438852596583535draftimg103732
66792Lentzea flava DSM 43885GCA_024171845scaffoldncbi103732

GC content

  • @ref: 67770
  • GC-content: 69
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno96.31no
gram-positiveyes88.889no
anaerobicno98.929no
aerobicyes90.604no
halophileno90.508no
spore-formingyes94.758no
thermophileno98.672yes
glucose-utilyes89.033yes
motileno91.73no
glucose-fermentno91.523no

External links

@ref: 11364

culture collection no.: DSM 43885, ATCC 29533, CIP 107110, INA 2171, JCM 3296, KCC A-0296, NCIB 11447, BCRC 13328, CGMCC 4.1715, IFO 14521, IMET 9748, IMSNU 21358, KCTC 9393, NBRC 14521, NCIMB 11447, NRRL B-16131, VKM Ac-906

straininfo link

  • @ref: 82498
  • straininfo: 39578

literature

  • topic: Phylogeny
  • Pubmed-ID: 11411672
  • title: Revival of the genus Lentzea and proposal for Lechevalieria gen. nov.
  • authors: Labeda DP, Hatano K, Kroppenstedt RM, Tamura T
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-3-1045
  • year: 2001
  • mesh: Actinomycetales/*classification/genetics/physiology, Carbohydrate Metabolism, Carboxylic Acids/metabolism, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11364Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43885)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43885
19612Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM43885.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20216Curators of the HKIhttp://www.leibniz-hki.de/de/Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI)
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40462Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4510
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68368Automatically annotated from API 20E
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
82498Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39578.1StrainInfo: A central database for resolving microbial strain identifiers
119364Curators of the CIPCollection of Institut Pasteur (CIP 107110)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107110