Strain identifier

BacDive ID: 132913

Type strain: Yes

Species: Enterococcus lemanii

Strain Designation: PC32

Strain history: <- CCUG; CCUG 61260 <- T. R. Whitehead and M. A. Cotta; PC32

NCBI tax ID(s): 1159752 (species)

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General

@ref: 24701

BacDive-ID: 132913

DSM-Number: 105069

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive

description: Enterococcus lemanii PC32 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from swine manure storage pit.

NCBI tax id

  • NCBI tax id: 1159752
  • Matching level: species

strain history

  • @ref: 24701
  • history: <- CCUG; CCUG 61260 <- T. R. Whitehead and M. A. Cotta; PC32

doi: 10.13145/bacdive132913.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus lemanii
  • full scientific name: Enterococcus lemanii Cotta et al. 2013

@ref: 24701

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus lemanii

full scientific name: Enterococcus lemanii Cotta et al. 2013

strain designation: PC32

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
24701COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
24701TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
24701positivegrowth37mesophilic
62596positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 62596
  • oxygen tolerance: microaerophile

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.674

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838129016arginine-hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol+builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose+builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
24701--+-+--+-+++++----++-----+------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
24701swine manure storage pitEureka near Peoria, IllinoisUSAUSANorth America
62596Swine manure stored in a deep pitEureka,IllinoisUSAUSANorth America1998-07-01

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Container (Reservoir)
#Host#Mammals#Suidae (Pig,Swine)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_31866.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_1097;97_3304;98_23654;99_31866&stattab=map
  • Last taxonomy: Enterococcus
  • 16S sequence: AF445301
  • Sequence Identity:
  • Total samples: 329
  • soil counts: 9
  • aquatic counts: 82
  • animal counts: 219
  • plant counts: 19

Safety information

risk assessment

  • @ref: 24701
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24701
  • description: Enterococcus lemanii strain PC32 16S ribosomal RNA gene, partial sequence
  • accession: AF445301
  • length: 1551
  • database: ena
  • NCBI tax ID: 1159752

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Enterococcus lemanii DSM 105069GCA_016909125contigncbi1159752
66792Enterococcus lemanii strain DSM 1050691159752.3wgspatric1159752
66792Enterococcus lemanii DSM 1050692901272077draftimg1159752

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno58no
motileyes52.632no
gram-positiveyes89.825no
anaerobicno94.201yes
aerobicno96.566yes
halophileyes59.636no
spore-formingno64.079no
flagellatedno79.883no
thermophileno96.632no
glucose-utilyes88.718no
glucose-fermentyes83.968no

External links

@ref: 24701

culture collection no.: DSM 105069, NRRL B-59661, CCM 8482, CCUG 61260

straininfo link

  • @ref: 91191
  • straininfo: 390260

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22872431Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit.Cotta MA, Whitehead TR, Falsen E, Moore E, Lawson PAAntonie Van Leeuwenhoek10.1007/s10482-012-9789-92012Animals, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Enterococcus/*classification/genetics/*isolation & purification/physiology, Fatty Acids/analysis, Manure/*microbiology, Molecular Chaperones/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, SwineGenetics
Phylogeny23592176Erratum to: Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit.Cotta MA, Whitehead TR, Falsen E, Moore E, Lawson PAAntonie Van Leeuwenhoek10.1007/s10482-013-9908-22013Animals, Bacterial Typing Techniques, Base Composition, Base Sequence, DNA, Bacterial/chemistry/genetics, Enterococcus/*classification/genetics/*isolation & purification/physiology, Fatty Acids/analysis, Feces/*microbiology, Manure/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/chemistry/genetics, Sequence Analysis, DNA, SwineGenetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24701Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105069Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105069)
62596Curators of the CCUGhttps://www.ccug.se/strain?id=61260Culture Collection University of Gothenburg (CCUG) (CCUG 61260)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
91191Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID390260.1