Strain identifier

BacDive ID: 132912

Type strain: Yes

Species: Enterococcus eurekensis

Strain Designation: PC4B

Strain history: <- CCUG; CCUG 61259 <- T. R. Whitehead and M. A. Cotta; PC4B

NCBI tax ID(s): 1159753 (species)

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General

@ref: 24700

BacDive-ID: 132912

DSM-Number: 105068

keywords: 16S sequence, Bacteria, microaerophile, mesophilic

description: Enterococcus eurekensis PC4B is a microaerophile, mesophilic bacterium that was isolated from swine manure storage pit.

NCBI tax id

  • NCBI tax id: 1159753
  • Matching level: species

strain history

  • @ref: 24700
  • history: <- CCUG; CCUG 61259 <- T. R. Whitehead and M. A. Cotta; PC4B

doi: 10.13145/bacdive132912.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Enterococcaceae
  • genus: Enterococcus
  • species: Enterococcus eurekensis
  • full scientific name: Enterococcus eurekensis Cotta et al. 2013

@ref: 24700

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Enterococcaceae

genus: Enterococcus

species: Enterococcus eurekensis

full scientific name: Enterococcus eurekensis Cotta et al. 2013

strain designation: PC4B

type strain: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
24700COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
24700TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
24700positivegrowth37mesophilic
62595positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 62595
  • oxygen tolerance: microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838129016arginine-hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose+builds acid from
6838127082trehalose+builds acid from
6838116634raffinose+builds acid from
6838117992sucrose+builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose+builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: no

metabolite tests

  • @ref: 68381
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase+3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase+3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
24700----+----++++-----++-----+------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
24700swine manure storage pitEureka near Peoria, IllinoisUSAUSANorth America
62595Swine manure stored in a deep pitIllinois,EurekaUSAUSANorth America1998-07-01

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Container (Reservoir)
#Host#Mammals#Suidae (Pig,Swine)
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Safety information

risk assessment

  • @ref: 24700
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24700
  • description: Enterococcus eurekensis strain PC4B 16S ribosomal RNA gene, partial sequence
  • accession: AF445305
  • length: 1551
  • database: ena
  • NCBI tax ID: 1159753

External links

@ref: 24700

culture collection no.: DSM 105068, CCUG 61259, NRRL B-59662, CCM 8483

straininfo link

  • @ref: 91190
  • straininfo: 390259

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22872431Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit.Cotta MA, Whitehead TR, Falsen E, Moore E, Lawson PAAntonie Van Leeuwenhoek10.1007/s10482-012-9789-92012Animals, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Enterococcus/*classification/genetics/*isolation & purification/physiology, Fatty Acids/analysis, Manure/*microbiology, Molecular Chaperones/genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, SwineGenetics
Phylogeny23592176Erratum to: Two novel species Enterococcus lemanii sp. nov. and Enterococcus eurekensis sp. nov., isolated from a swine-manure storage pit.Cotta MA, Whitehead TR, Falsen E, Moore E, Lawson PAAntonie Van Leeuwenhoek10.1007/s10482-013-9908-22013Animals, Bacterial Typing Techniques, Base Composition, Base Sequence, DNA, Bacterial/chemistry/genetics, Enterococcus/*classification/genetics/*isolation & purification/physiology, Fatty Acids/analysis, Feces/*microbiology, Manure/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/chemistry/genetics, Sequence Analysis, DNA, SwineGenetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24700Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-105068Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 105068)
62595Curators of the CCUGhttps://www.ccug.se/strain?id=61259Culture Collection University of Gothenburg (CCUG) (CCUG 61259)
68381Automatically annotated from API rID32STR
91190Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID390259.1