Strain identifier

BacDive ID: 132681

Type strain: Yes

Species: Falcatimonas natans

Strain Designation: WN011

Strain history: A. Ueki; Fac. of Agric., Yamagata Univ., Japan; WN011.

NCBI tax ID(s): 433279 (species)

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General

@ref: 24470

BacDive-ID: 132681

DSM-Number: 22923

keywords: 16S sequence, Bacteria, anaerobe, chemoorganotroph, Gram-negative, motile, rod-shaped, colony-forming

description: Falcatimonas natans WN011 is an anaerobe, chemoorganotroph, Gram-negative bacterium that forms circular colonies and was isolated from rice-straw residue in a methanogenic reactor treating waste from cattle farms.

NCBI tax id

  • NCBI tax id: 433279
  • Matching level: species

strain history

@refhistory
24470<- A. Ueki, Yamagata Univ., Tsuruoka, Japan; WN011 <- A. Ueki {2005}
67770A. Ueki; Fac. of Agric., Yamagata Univ., Japan; WN011.

doi: 10.13145/bacdive132681.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Lachnospiraceae
  • genus: Falcatimonas
  • species: Falcatimonas natans
  • full scientific name: Falcatimonas natans Watanabe et al. 2016

@ref: 24470

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Lachnospiraceae

genus: Falcatimonas

species: Falcatimonas natans

full scientific name: Falcatimonas natans Watanabe et al. 2016

strain designation: WN011

type strain: yes

Morphology

cell morphology

  • @ref: 25038
  • gram stain: negative
  • cell length: 1.3-2.3 µm
  • cell width: 0.4-0.6 µm
  • cell shape: rod-shaped
  • motility: yes
  • flagellum arrangement: polar

colony morphology

  • @ref: 25038
  • colony size: 0.5-1.0 mm
  • colony shape: circular
  • medium used: PY4S agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
25038PY4S agaryes
24470PY + X MEDIUM (DSMZ Medium 104b)yeshttps://mediadive.dsmz.de/medium/104bName: PY + X MEDIUM (DSMZ Medium 104b) Composition: Yeast extract 10.0 g/l D-Glucose 5.0 g/l Trypticase peptone 5.0 g/l Meat peptone 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l KH2PO4 0.04 g/l K2HPO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Sodium resazurin 0.0005 g/l Distilled water

culture temp

@refgrowthtypetemperature
25038positivegrowth15-40
25038positiveoptimum35-37
24470positivegrowth30
67770positivegrowth30

culture pH

@refabilitytypepH
25038positivegrowth5.8-7.5
25038positiveoptimum6.7

Physiology and metabolism

oxygen tolerance

  • @ref: 25038
  • oxygen tolerance: anaerobe

nutrition type

  • @ref: 25038
  • type: chemoorganotroph

halophily

@refsaltgrowthtested relationconcentration
25038NaClpositivegrowth0-20.0 g/L
25038NaCloptimum0 g/L

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2503829991L-aspartate-carbon source
2503829985L-glutamate-carbon source
2503827613amygdalin-carbon source
2503822599arabinose-carbon source
2503822599arabinose-builds acid from
2503885146carboxymethylcellulose-carbon source
2503817057cellobiose-carbon source
2503862968cellulose-carbon source
250384853esculin-carbon source
2503828260galactose-carbon source
2503817234glucose-carbon source
2503817234glucose-builds acid from
2503828087glycogen-carbon source
2503817268myo-inositol-carbon source
2503815443inulin-carbon source
2503816977L-alanine-carbon source
2503817196L-asparagine-carbon source
2503818050L-glutamine-carbon source
2503815971L-histidine-carbon source
2503816643L-methionine-carbon source
2503817295L-phenylalanine-carbon source
2503817203L-proline-carbon source
2503816828L-tryptophan-carbon source
2503817716lactose-builds acid from
2503817306maltose-carbon source
2503817306maltose-builds acid from
2503829864mannitol-carbon source
2503837684mannose-carbon source
250386731melezitose-carbon source
2503828053melibiose-carbon source
2503817632nitrate-reduction
2503817309pectin-carbon source
2503816634raffinose-carbon source
2503826546rhamnose-carbon source
2503833942ribose-carbon source
2503817814salicin-carbon source
2503830911sorbitol-carbon source
2503828017starch-carbon source
2503817992sucrose-carbon source
2503817992sucrose-builds acid from
2503827082trehalose-carbon source
2503827897tryptophan-energy source
2503837166xylan-carbon source
2503818222xylose-carbon source
2503818222xylose-builds acid from
250384853esculin+hydrolysis
2503817191L-isoleucine+carbon source
2503815603L-leucine+carbon source
2503818019L-lysine+carbon source
2503817115L-serine+carbon source
2503816857L-threonine+carbon source
2503816414L-valine+carbon source

metabolite production

  • @ref: 25038
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 25038
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
25038catalase-1.11.1.6
25038cytochrome oxidase-1.9.3.1
25038lecithinase-
25038lipase-
25038tryptophan deaminase-4.1.99.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentenrichment cultureenrichment culture temperature
24470rice-straw residue in a methanogenic reactor treating waste from cattle farmsHokkaido, Betsukai-machiJapanJPNAsia
25038peptone/yeast extract (PY)30
67770Methanogenic reactor of cattle waste

isolation source categories

Cat1Cat2Cat3
#Engineered#Bioreactor
#Engineered#Waste
#Host#Mammals#Bovinae (Cow, Cattle)
#Host#Plants#Herbaceous plants (Grass,Crops)

taxonmaps

  • @ref: 69479
  • File name: preview.99_150218.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_16;96_17802;97_21940;98_27545;99_150218&stattab=map
  • Last taxonomy: Falcatimonas natans subclade
  • 16S sequence: AB298726
  • Sequence Identity:
  • Total samples: 9947
  • soil counts: 362
  • aquatic counts: 5900
  • animal counts: 3510
  • plant counts: 175

Safety information

risk assessment

  • @ref: 24470
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24470
  • description: Falcatimonas natans gene for 16S rRNA, partial sequence
  • accession: AB298726
  • length: 1484
  • database: nuccore
  • NCBI tax ID: 433279

GC content

  • @ref: 24470
  • GC-content: 38.8
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24470

culture collection no.: DSM 22923, JCM 16476

straininfo link

  • @ref: 90989
  • straininfo: 397706

literature

  • topic: Phylogeny
  • Pubmed-ID: 27506535
  • title: Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste.
  • authors: Watanabe M, Kaku N, Ueki K, Ueki A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001403
  • year: 2016
  • mesh: Animals, Bacteria, Anaerobic/classification, Bacterial Typing Techniques, Base Composition, Bioreactors/*microbiology, Cattle/*microbiology, Clostridiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Fermentation, Japan, Manure/*microbiology, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24470Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-22923Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 22923)
25038M. K. Watanabe, N.,Ueki, K.,Ueki, A.Falcatimonas natans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor of cattle waste10.1099/ijsem.0.001403IJSEM 66: 4639-4644 201627506535
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
90989Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397706.1