Strain identifier

BacDive ID: 132661

Type strain: Yes

Species: Brachybacterium aquaticum

Strain Designation: KWS-1

Strain history: S. Mayilraj; MTCC, India; KWS-1.

NCBI tax ID(s): 1432564 (species)

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General

@ref: 24450

BacDive-ID: 132661

DSM-Number: 28796

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, coccus-shaped, colony-forming, pigmented

description: Brachybacterium aquaticum KWS-1 is an aerobe, mesophilic, Gram-positive bacterium that forms circular colonies and has a pale-yellowish pigmentation.

NCBI tax id

  • NCBI tax id: 1432564
  • Matching level: species

strain history

@refhistory
24450<- M. Shanmugam, MTCC; KWS-1 <- S. Mayilraj, MTCC; MTCC 11836
67770S. Mayilraj; MTCC, India; KWS-1.

doi: 10.13145/bacdive132661.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Dermabacteraceae
  • genus: Brachybacterium
  • species: Brachybacterium aquaticum
  • full scientific name: Brachybacterium aquaticum Kaur et al. 2016

@ref: 24450

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Dermabacteraceae

genus: Brachybacterium

species: Brachybacterium aquaticum

full scientific name: Brachybacterium aquaticum Kaur et al. 2016

strain designation: KWS-1

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
24973positivecoccus-shaped
69480no90.699
69480positive100

colony morphology

  • @ref: 24973
  • colony size: 0.4-3.0 mm
  • colony color: straw-yellow
  • colony shape: circular
  • medium used: TSA (Trypticase soy agar)

pigmentation

  • @ref: 24973
  • production: yes
  • color: pale-yellowish

Culture and growth conditions

culture medium

  • @ref: 24973
  • name: TSA (Trypticase soy agar)
  • growth: yes

culture temp

@refgrowthtypetemperaturerange
24973positiveoptimum30mesophilic
24973positivegrowth20.0-37.0
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
24973positiveoptimum8
24973positivegrowth7.0-11.0alkaliphile
24973nogrowth5
24973positivegrowth11

Physiology and metabolism

oxygen tolerance

  • @ref: 24973
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
69481no99
69480no99.956

halophily

@refsaltgrowthtested relationconcentration
24973NaClpositivemaximum8 %(w/v)
24973NaClpositivegrowth5 %(w/v)
24973NaClpositivegrowth7 %(w/v)

murein

  • @ref: 24973
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-7

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2497317019(R)-amygdalin-builds acid from
24973casein-hydrolysis
2497317057cellobiose-builds acid from
2497323456cyclodextrin-builds acid from
2497312936D-galactose-builds acid from
2497316899D-mannitol-builds acid from
2497316024D-mannose-builds acid from
2497316988D-ribose-builds acid from
2497317924D-sorbitol-builds acid from
2497365327D-xylose-builds acid from
249735291gelatin-hydrolysis
2497317716lactose-builds acid from
2497317306maltose-builds acid from
24973320055methyl beta-D-glucopyranoside-builds acid from
24973506227N-acetylglucosamine-builds acid from
2497317632nitrate-reduction
24973O-nitrophenyl-beta-D-galactopyranosid-hydrolysis
2497327941pullulan-builds acid from
2497316634raffinose-builds acid from
2497326546rhamnose-builds acid from
2497317814salicin-builds acid from
2497317992sucrose-builds acid from
2497327082trehalose-builds acid from
2497327897tryptophan-energy source
2497328757fructose+builds acid from
2497328260galactose+builds acid from
2497317234glucose+builds acid from
2497328017starch+hydrolysis
2497317992sucrose+builds acid from

antibiotic resistance

@refChEBImetaboliteis antibioticis resistant
249738309polymyxin byesno
2497328669bacitracinyesno
2497328368novobiocinyesno
2497386455optochinyesno

metabolite production

@refChebi-IDmetaboliteproduction
2497315688acetoinno
2497316136hydrogen sulfideno
2497335581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
2497315688acetoin-
2497335581indole-

enzymes

@refvalueactivityec
24973alanine arylamidase+3.4.11.2
24973alpha-galactosidase-3.2.1.22
24973alpha-glucosidase-3.2.1.20
24973alpha-mannosidase-3.2.1.24
24973arginine dihydrolase+3.5.3.6
24973beta-galactosidase+3.2.1.23
24973beta-glucuronidase-3.2.1.31
24973catalase+1.11.1.6
24973cytochrome oxidase-1.9.3.1
24973L-aspartate arylamidase-3.4.11.21
24973phosphatase-
24973proline-arylamidase+3.4.11.5
24973pyrrolidonyl arylamidase-3.4.19.3
24973tripeptide aminopeptidase-3.4.11.4
24973tryptophan deaminase-4.1.99.1
24973tyrosine arylamidase+
24973urease-3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureisolation procedure
24450seawaterKanyakumari coastal region of the Bay of Bengal, Kovalam (8.0914651° N 77.5184862° E)IndiaINDAsia8.091577.5185
24973TSA (Trypticase soy agar)(HiMedia)dilution-plate technique
67770Seawater from the Kanyakumari coastal region of the Bay of BengalIndiaINDAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic
  • Cat3: #Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_11726.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_9;96_1217;97_1443;98_1761;99_11726&stattab=map
  • Last taxonomy: Brachybacterium aquaticum
  • 16S sequence: KF701619
  • Sequence Identity:
  • Total samples: 233
  • soil counts: 11
  • aquatic counts: 40
  • animal counts: 173
  • plant counts: 9

Sequence information

16S sequences

  • @ref: 24450
  • description: Brachybacterium aquaticum strain KWS1 16S ribosomal RNA gene, partial sequence
  • accession: KF701619
  • length: 1423
  • database: ena
  • NCBI tax ID: 1432564

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brachybacterium aquaticum DSM 28796GCA_014204755contigncbi1432564
66792Brachybacterium aquaticum strain DSM 287961432564.3wgspatric1432564
66792Brachybacterium aquaticum DSM 287962868155942draftimg1432564

GC content

@refGC-contentmethod
2497371.8Thermal denaturation, fluorometry
2445071.8thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileno93.373no
gram-positiveyes94.345yes
anaerobicno99.21yes
halophileno52.41yes
spore-formingno93.855no
thermophileno99.029yes
glucose-utilyes88.177no
aerobicyes81.395no
flagellatedno98.189no
glucose-fermentno64.35no

External links

@ref: 24450

culture collection no.: DSM 28796, JCM 30059, MTCC 11836

literature

  • topic: Phylogeny
  • Pubmed-ID: 27514821
  • title: Brachybacterium aquaticum sp. nov., a novel actinobacterium isolated from seawater.
  • authors: Kaur G, Kumar N, Mual P, Kumar A, Kumar RM, Mayilraj S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001414
  • year: 2016
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/chemistry, India, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24450Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28796Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28796)
24973G. K. Kaur, Narender,Mual, Poonam,Kumar, Anand,Kumar, Rajendran Mathan,Mayilraj, ShanmugamBrachybacterium aquaticum sp. nov., a novel actinobacterium isolated from seawater10.1099/ijsem.0.001414IJSEM 66: 4705-4710 201627514821
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1