Strain identifier

BacDive ID: 132631

Type strain: Yes

Species: Jatrophihabitans huperziae

Strain Designation: I13A-01604

Strain history: <- Y.-Q. Zhang; CPCC 204076 <- H.-M. Sun, Chinese Academy of Medical Sciences & Peking Union Medical College, China

NCBI tax ID(s): 1730378 (species)

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General

@ref: 24420

BacDive-ID: 132631

DSM-Number: 46866

keywords: 16S sequence, Bacteria, Gram-positive, rod-shaped

description: Jatrophihabitans huperziae I13A-01604 is a Gram-positive, rod-shaped bacterium that was isolated from surface-sterilized tissue of the medicinal plant Huperzia serrata .

NCBI tax id

  • NCBI tax id: 1730378
  • Matching level: species

strain history

  • @ref: 24420
  • history: <- Y.-Q. Zhang; CPCC 204076 <- H.-M. Sun, Chinese Academy of Medical Sciences & Peking Union Medical College, China

doi: 10.13145/bacdive132631.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Jatrophihabitantales
  • family: Jatrophihabitantaceae
  • genus: Jatrophihabitans
  • species: Jatrophihabitans huperziae
  • full scientific name: Jatrophihabitans huperziae Gong et al. 2016

@ref: 24420

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Jatrophihabitantaceae

genus: Jatrophihabitans

species: Jatrophihabitans huperziae

full scientific name: Jatrophihabitans huperziae Gong et al. 2016 emend. Lee et al. 2018

strain designation: I13A-01604

type strain: yes

Morphology

cell morphology

  • @ref: 24895
  • gram stain: positive
  • cell length: 1.0-1.1 µm
  • cell width: 0.5-0.7 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 24895
  • colony color: soft orange

multimedia

  • @ref: 24420
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_46866.jpg
  • caption: Medium 535 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
24420R2A MEDIUM (DSMZ Medium 830)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium830.pdf
24420TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
24895positivegrowth10-37
24895positiveoptimum32mesophilic
24420positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
24895positivegrowth5.0-9.0alkaliphile
24895positiveoptimum7.0-7.5

Physiology and metabolism

spore formation

  • @ref: 24895
  • spore formation: no

halophily

  • @ref: 24895
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-3 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2489562968cellulose-hydrolysis
248955291gelatin-hydrolysis
2489517234glucose-fermentation
2489527897tryptophan-energy source
2489516199urea-hydrolysis
2489515588D-malate+respiration
2489530089acetate+respiration
2489513705acetoacetate+respiration
2489517925alpha-D-glucose+respiration
2489536219alpha-lactose+respiration
2489517057cellobiose+respiration
2489516947citrate+respiration
2489518333D-arabitol+respiration
2489529990D-aspartate+respiration
2489515824D-fructose+respiration
2489578697D-fructose 6-phosphate+respiration
2489528847D-fucose+respiration
2489512936D-galactose+respiration
2489518024D-galacturonic acid+respiration
248958391D-gluconate+respiration
2489514314D-glucose 6-phosphate+respiration
2489515748D-glucuronate+respiration
2489516899D-mannitol+respiration
2489516024D-mannose+respiration
2489517924D-sorbitol+respiration
2489528066gentiobiose+respiration
2489532323glucuronamide+respiration
2489517754glycerol+respiration
2489516977L-alanine+respiration
2489516467L-arginine+respiration
2489517115L-serine+respiration
2489517306maltose+respiration
2489528053melibiose+respiration
2489574611methyl (R)-lactate+respiration
2489528017starch+hydrolysis
2489553423tween 40+hydrolysis
2489553423tween 40+respiration
2489553426tween 80+hydrolysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.
248952637amikacinyesyes30 µg/mL
2489528971ampicillinyesyes10 µg/mL
24895161680aztreonamyesyes30 µg/mL
2489548923erythromycinyesyes15 µg/mL
2489517833gentamicinyesyes10 µg/mL
248956104kanamycinyesyes100 µg/mL
2489518208penicillin gyesyes10 Unit
2489517076streptomycinyesyes10 µg/mL
2489528864tobramycinyesyes10 µg/mL

metabolite production

@refChebi-IDmetaboliteproduction
2489515688acetoinno
2489535581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testindole test
2489517234glucose-
2489515688acetoin-
2489535581indole-

enzymes

@refvalueactivityec
24895acid phosphatase+3.1.3.2
24895alkaline phosphatase+3.1.3.1
24895alpha-glucosidase+3.2.1.20
24895beta-glucuronidase+3.2.1.31
24895catalase-1.11.1.6
24895cytochrome oxidase+1.9.3.1
24895esterase (C 4)+
24895esterase lipase (C 8)+
24895leucine arylamidase+3.4.11.1
24895tryptophan deaminase-4.1.99.1

Isolation, sampling and environmental information

isolation

  • @ref: 24420
  • sample type: surface-sterilized tissue of the medicinal plant Huperzia serrata (Thunb.)
  • host species: Huperzia serrata
  • geographic location: Sichuan province, Hongya County, Emeishan area of (29° 41' 24.98'' N 103° 10' 16.23'' E, 1187 m altitude)
  • country: China
  • origin.country: CHN
  • continent: Asia
  • latitude: 29.6903
  • longitude: 103.171

isolation source categories

Cat1Cat2Cat3
#Host#Plants#Herbaceous plants (Grass,Crops)
#Host Body-Site#Plant#Sterilized plant part

Safety information

risk assessment

  • @ref: 24420
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24420
  • description: Jatrophihabitans huperziae strain I13A-01604 16S ribosomal RNA gene, partial sequence
  • accession: KR184574
  • length: 1493
  • database: ena
  • NCBI tax ID: 1730378

GC content

@refGC-contentmethod
2489571.9Thermal denaturation, fluorometry
2442071.9thermal denaturation, midpoint method (Tm)

External links

@ref: 24420

culture collection no.: DSM 46866, CPCC 204076, NBRC 110718

straininfo link

  • @ref: 90945
  • straininfo: 405482

literature

  • topic: Phylogeny
  • Pubmed-ID: 27413046
  • title: Jatrophihabitans huperziae sp. nov., an endophytic actinobacterium isolated from surface-sterilized tissue of the medicinal plant Huperzia serrata (Thunb.).
  • authors: Gong ZL, Ai MJ, Sun HM, Liu HY, Yu LY, Zhang YQ
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001296
  • year: 2016
  • mesh: Actinobacteria/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Glycolipids/chemistry, Huperzia/*microbiology, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, Plants, Medicinal/microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24420Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-46866Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46866)
24895Z.-L. A. Gong, Meng-Jie,Sun, Hong-Min,Liu, Hong-Yu,Yu, Li-Yan,Zhang, Yu-QinJatrophihabitans huperziae sp. nov., an endophytic actinobacterium isolated from surface-sterilized tissue of the medicinal plant Huperzia serrata (Thunb.)10.1099/ijsem.0.001296IJSEM 66: 3972-3977 201627413046
90945Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405482.1