Strain identifier
BacDive ID: 132625
Type strain: 
Species: Neisseria musculi
Strain Designation: AP2031
Strain history: <- N. J. Weyand, Univ. of Arizona; AP2031
NCBI tax ID(s): 1815583 (species)
version 8.1 (current version)
General
@ref: 24414
BacDive-ID: 132625
DSM-Number: 101846
keywords: Bacteria, microaerophile, mesophilic, Gram-negative, colony-forming
description: Neisseria musculi AP2031 is a microaerophile, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from oral cavity of a healthy wild caught house mouse Mus musculus domesticus.
NCBI tax id
- NCBI tax id: 1815583
- Matching level: species
strain history
- @ref: 24414
- history: <- N. J. Weyand, Univ. of Arizona; AP2031
doi: 10.13145/bacdive132625.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Betaproteobacteria
- order: Neisseriales
- family: Neisseriaceae
- genus: Neisseria
- species: Neisseria musculi
- full scientific name: Neisseria musculi Weyand et al. 2016
@ref: 24414
domain: Bacteria
phylum: Proteobacteria
class: Betaproteobacteria
order: Neisseriales
family: Neisseriaceae
genus: Neisseria
species: Neisseria musculi
full scientific name: Neisseria musculi Weyand et al. 2016
strain designation: AP2031
type strain: yes
Morphology
cell morphology
- @ref: 43162
- gram stain: negative
- cell length: 0.5-0.8 µm
- cell width: 0.4 µm
colony morphology
- @ref: 43162
- colony size: 0.5-1.0 mm
- colony shape: circular
- incubation period: 2 days
- medium used: GCB agar
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 43162 | chocolate agar | yes | ||
| 43162 | TSA with 5% sheep blood | yes | ||
| 43162 | GCB agar | yes | ||
| 24414 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 63466 | positive | growth | 37 | mesophilic |
| 24414 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 63466
- oxygen tolerance: microaerophile
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68377 | 15824 | D-fructose | + | builds acid from |
| 68377 | 17992 | sucrose | + | builds acid from |
| 68377 | 16199 | urea | - | hydrolysis |
| 68377 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 43162 | cytochrome oxidase | + | 1.9.3.1 |
| 68377 | tryptophan deaminase | - | 4.1.99.1 |
| 68377 | gamma-glutamyltransferase | - | 2.3.2.2 |
| 68377 | proline-arylamidase | - | 3.4.11.5 |
| 68377 | beta-galactosidase | - | 3.2.1.23 |
| 68377 | alkaline phosphatase | - | 3.1.3.1 |
| 68377 | lipase | - | |
| 68377 | urease | - | 3.5.1.5 |
API NH
| @ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL | ProA | GGT | IND |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 24414 | + | - | + | - | + | - | - | - | - | - | - | - | |
| 24414 | - | + | + | + | + | + | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
| @ref | sample type | host species | geographic location | country | origin.country | continent | latitude | longitude | sampling date |
|---|---|---|---|---|---|---|---|---|---|
| 24414 | oral cavity of a healthy wild caught house mouse Mus musculus domesticus | Mus musculus domesticus | Arizona, Tucson | USA | USA | North America | 32.2088 | -110.921 | |
| 43162 | Tucson Arizona, USA | USA | USA | North America | |||||
| 63466 | Oral cavity of of a wild caught house mouse | Tucson Arizona | USA | USA | North America | 2012-10-10 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Mammals | #Muridae (Mouse/Rat) |
| #Host Body-Site | #Oral cavity and airways |
Safety information
risk assessment
- @ref: 24414
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
GC content
| @ref | GC-content | method |
|---|---|---|
| 43162 | 53.3 | |
| 24414 | 53.3 | sequence analysis |
External links
@ref: 24414
culture collection no.: DSM 101846, CCUG 68283, LMG 29261
straininfo link
- @ref: 90939
- straininfo: 402879
literature
- topic: Phylogeny
- Pubmed-ID: 27298306
- title: Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse.
- authors: Weyand NJ, Ma M, Phifer-Rixey M, Taku NA, Rendon MA, Hockenberry AM, Kim WJ, Agellon AB, Biais N, Suzuki TA, Goodyer-Sait L, Harrison OB, Bratcher HB, Nachman MW, Maiden MCJ, So M
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001237
- year: 2016
- mesh: Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Genes, Bacterial, Mice/*microbiology, Mouth/microbiology, Multilocus Sequence Typing, Neisseria/*classification/genetics/isolation & purification, North America, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Transcriptome
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 24414 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-101846 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101846) | |||
| 43162 | Nathan J. Weyand, Mancheong Ma, Megan Phifer-Rixey, Nyiawung A. Taku, María A. Rendón, Alyson M. Hockenberry, Won J. Kim, Al B. Agellon, Nicolas Biais, Taichi A. Suzuki, Lily Goodyer-Sait, Odile B. Harrison, Holly B. Bratcher, Michael W. Nachman, Martin C. J. Maiden and Magdalene So | Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse | 10.1099/ijsem.0.001237 | IJSEM 66: 3585-3593 2016 | 27298306 | |
| 63466 | Curators of the CCUG | https://www.ccug.se/strain?id=68283 | Culture Collection University of Gothenburg (CCUG) (CCUG 68283) | |||
| 68377 | Automatically annotated from API NH | |||||
| 90939 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402879.1 |