Strain identifier

BacDive ID: 132623

Type strain: Yes

Species: Sulfuriflexus mobilis

Strain history: <- H. Kojima, Hokkaido University, Sapporo, Japan <- H. Kojima {2015}

NCBI tax ID(s): 1811807 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 9 (current version):
version 8.1:
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 9 (current version)

General

@ref: 24412

BacDive-ID: 132623

DSM-Number: 102939

keywords: 16S sequence, Bacteria, anaerobe, chemolithoautotroph, Gram-negative, motile

description: Sulfuriflexus mobilis DSM 102939 is an anaerobe, chemolithoautotroph, Gram-negative bacterium that was isolated from sediment of a brackish lake.

NCBI tax id

  • NCBI tax id: 1811807
  • Matching level: species

strain history

  • @ref: 24412
  • history: <- H. Kojima, Hokkaido University, Sapporo, Japan <- H. Kojima {2015}

doi: 10.13145/bacdive132623.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Chromatiales
  • family: Granulosicoccaceae
  • genus: Sulfuriflexus
  • species: Sulfuriflexus mobilis
  • full scientific name: Sulfuriflexus mobilis Kojima and Fukui 2016

@ref: 24412

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Chromatiales

family: Granulosicoccaceae

genus: Sulfuriflexus

species: Sulfuriflexus mobilis

full scientific name: Sulfuriflexus mobilis Kojima and Fukui 2016

type strain: yes

Morphology

cell morphology

  • @ref: 43156
  • gram stain: negative
  • cell length: 0.9-6.0 µm
  • cell width: 0.3-0.5 µm
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43156MBno
24412SULFURIFLEXUS MEDIUM (DSMZ Medium 1635)yeshttps://mediadive.dsmz.de/medium/1635Name: SULFURIFLEXUS MEDIUM (DSMZ Medium 1635) Composition: NaCl 19.9402 g/l Na2S2O3 x 5 H2O 4.98504 g/l MgCl2 x 6 H2O 2.99103 g/l NaHCO3 0.398804 g/l MgSO4 x 7 H2O 0.299103 g/l KCl 0.0997009 g/l KH2PO4 0.0997009 g/l NH4Cl 0.0997009 g/l CaCl2 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

@refgrowthtypetemperature
43156positiveoptimum30-32
43156positivegrowth5-34
24412positivegrowth28

culture pH

@refabilitytypepHPH range
43156positivegrowth6.4-8.7alkaliphile
43156positiveoptimum6.8-8.3

Physiology and metabolism

oxygen tolerance

  • @ref: 43156
  • oxygen tolerance: anaerobe

nutrition type

@reftype
43156chemolithoautotroph
43156autotroph

halophily

@refsaltgrowthtested relationconcentration
43156NaClpositivegrowth1-4 %
43156NaClpositiveoptimum3 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4315616094thiosulfate+oxidation
4315615138sulfide+oxidation
4315633403elemental sulfur+oxidation
4315615361pyruvate-growth
4315624996lactate-growth
4315630089acetate-growth
4315617790methanol-growth
4315630031succinate-growth
4315629806fumarate-growth
4315617968butyrate-growth
4315648944isobutyrate-growth
4315616236ethanol-growth
4315615740formate-growth
4315617716lactose-growth
4315617234glucose-growth
4315618222xylose-growth

enzymes

@refvalueactivityec
43156cytochrome oxidase+1.9.3.1
43156catalase-1.11.1.6

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43156C16:1ω7c / C16:1ω6c57.3
    43156C16:032.2
    43156iso-C17:1ω7c / 10-methyl C16:05.2
    43156C18:1ω7c / C18:1ω6c1.7
    43156C10:0 3OH1.3
    43156C18:00.8
    43156C14:00.6
    43156C12:0 3OH0.4
    43156C17:00.3
    43156C10:00.2
  • type of FA analysis: whole cell analysis
  • incubation medium: basal medium supplemented with 20mM Na2S2O3
  • agar/liquid: liquid
  • incubation temperature: 30
  • software version: Sherlock 6.0
  • system: MIS MIDI

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
24412sediment of a brackish lakeLake AkkeshiJapanJPNAsia
43156sediment of the Lake AkkeshiLake Akkeshi, JapanJapanJPNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Brackish
#Environmental#Aquatic#Lake (large)
#Environmental#Aquatic#Sediment

Safety information

risk assessment

  • @ref: 24412
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24412
  • description: Sulfuriflexus mobilis gene for 16S ribosomal RNA, partial sequence
  • accession: LC131141
  • length: 1464
  • database: nuccore
  • NCBI tax ID: 1811807

GC content

  • @ref: 24412
  • GC-content: 53
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 24412

culture collection no.: DSM 102939, NBRC 111889, aks 1

straininfo link

  • @ref: 90937
  • straininfo: 407384

literature

  • topic: Phylogeny
  • Pubmed-ID: 27282620
  • title: Sulfuriflexusmobilis gen. nov., sp. nov., a sulfur-oxidizing bacterium isolated from a brackish lake sediment.
  • authors: Kojima H, Fukui M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001227
  • year: 2016
  • mesh: Bacterial Typing Techniques, Base Composition, Chemoautotrophic Growth, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gammaproteobacteria/*classification/genetics/isolation & purification, Japan, Lakes/*microbiology, Oxidation-Reduction, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sulfur/metabolism, Sulfur-Reducing Bacteria/*classification/genetics/isolation & purification
  • topic2: Enzymology

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24412Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-102939Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102939)
43156Hisaya Kojima and Manabu FukuiSulfuriflexus mobilis gen. nov., sp. nov., a sulfur-oxidizing bacterium isolated from a brackish lake sediment10.1099/ijsem.0.001227IJSEM 66: 3515-3518 201627282620
90937Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID407384.1