Strain identifier
BacDive ID: 132604
Type strain:
Species: Tsukamurella serpentis
Strain Designation: HKU54
Strain history: J. L. L. Teng; Dept. of Microbiol., Univ. of Hong Kong, China; HKU54.
NCBI tax ID(s): 1750603 (species)
General
@ref: 24393
BacDive-ID: 132604
DSM-Number: 100915
keywords: 16S sequence, Bacteria, aerobe, thermophilic, Gram-positive, rod-shaped, colony-forming
description: Tsukamurella serpentis HKU54 is an aerobe, thermophilic, Gram-positive bacterium that forms irregular colonies and was isolated from oral cavity of a healthy Chinese cobra Naja atra.
NCBI tax id
- NCBI tax id: 1750603
- Matching level: species
strain history
@ref | history |
---|---|
24393 | <- J. L. L. Teng, Univ. of Hong Kong; HKU54 |
67770 | J. L. L. Teng; Dept. of Microbiol., Univ. of Hong Kong, China; HKU54. |
doi: 10.13145/bacdive132604.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Tsukamurellaceae
- genus: Tsukamurella
- species: Tsukamurella serpentis
- full scientific name: Tsukamurella serpentis Tang et al. 2016
@ref: 24393
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Tsukamurellaceae
genus: Tsukamurella
species: Tsukamurella serpentis
full scientific name: Tsukamurella serpentis Tang et al. 2016
strain designation: HKU54
type strain: yes
Morphology
cell morphology
- @ref: 43103
- gram stain: positive
- cell shape: rod-shaped
- motility: no
colony morphology
- @ref: 43103
- colony size: 2 mm
- colony color: cream-to-yellow
- colony shape: irregular
- incubation period: 2 days
- medium used: Columbia agar with 5 % defibrinated sheep blood agar
multimedia
- @ref: 24393
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_100915.jpg
- caption: Medium 693 25°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
43103 | Columbia agar with 5 % defibrinated sheep blood agar | yes | ||
43103 | brain-heart infusion agar | yes | ||
43103 | nutritient agar (NA) | yes | ||
43103 | trypticase soy agar (TSA) | yes | ||
43103 | chocolate agar | yes | ||
43103 | MacConkey agar | yes | ||
24393 | TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) | yes | https://mediadive.dsmz.de/medium/535a | Name: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
43103 | no | growth | 42 | thermophilic |
43103 | positive | growth | 10-37 | |
24393 | positive | growth | 25 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 43103
- oxygen tolerance: aerobe
spore formation
- @ref: 43103
- spore formation: no
halophily
- @ref: 43103
- salt: NaCl
- growth: positive
- tested relation: maximum
- concentration: 7 %(w/v)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
43103 | 15318 | xanthine | - | hydrolysis |
43103 | 18186 | tyrosine | - | hydrolysis |
43103 | 12936 | D-galactose | + | assimilation |
43103 | 17634 | D-glucose | + | assimilation |
43103 | 15824 | D-fructose | + | assimilation |
43103 | 17268 | myo-inositol | + | assimilation |
43103 | 16899 | D-mannitol | + | assimilation |
43103 | 17924 | D-sorbitol | + | assimilation |
43103 | 17306 | maltose | + | assimilation |
43103 | 17992 | sucrose | + | assimilation |
43103 | 27082 | trehalose | + | assimilation |
43103 | 17151 | xylitol | + | assimilation |
43103 | 32528 | turanose | + | assimilation |
43103 | 18333 | D-arabitol | + | assimilation |
43103 | 17108 | D-arabinose | - | assimilation |
43103 | 30849 | L-arabinose | - | assimilation |
43103 | 16988 | D-ribose | - | assimilation |
43103 | 65327 | D-xylose | - | assimilation |
43103 | 16813 | galactitol | - | assimilation |
43103 | 37657 | methyl D-glucoside | - | assimilation |
43103 | 506227 | N-acetylglucosamine | - | assimilation |
43103 | esculin ferric citrate | - | assimilation | |
43103 | 17814 | salicin | - | assimilation |
43103 | 17057 | cellobiose | - | assimilation |
43103 | 28053 | melibiose | - | assimilation |
43103 | 6731 | melezitose | - | assimilation |
43103 | 18287 | L-fucose | - | assimilation |
43103 | 32032 | potassium gluconate | - | assimilation |
43103 | 16808 | 2-dehydro-D-gluconate | - | assimilation |
43103 | 65328 | L-xylose | - | assimilation |
43103 | 15963 | ribitol | - | assimilation |
43103 | 17266 | L-sorbose | - | assimilation |
43103 | 62345 | L-rhamnose | - | assimilation |
43103 | 43943 | methyl alpha-D-mannoside | - | assimilation |
43103 | 27613 | amygdalin | - | assimilation |
43103 | 18305 | arbutin | - | assimilation |
43103 | 17716 | lactose | - | assimilation |
43103 | 15443 | inulin | - | assimilation |
43103 | 16634 | raffinose | - | assimilation |
43103 | 28017 | starch | - | assimilation |
43103 | 28087 | glycogen | - | assimilation |
43103 | 28066 | gentiobiose | - | assimilation |
43103 | 62318 | D-lyxose | - | assimilation |
43103 | 16443 | D-tagatose | - | assimilation |
43103 | 28847 | D-fucose | - | assimilation |
43103 | 18403 | L-arabitol | - | assimilation |
43103 | 58143 | 5-dehydro-D-gluconate | - | assimilation |
enzymes
@ref | value | activity | ec |
---|---|---|---|
43103 | catalase | + | 1.11.1.6 |
43103 | cytochrome oxidase | - | 1.9.3.1 |
fatty acid profile
fatty acids
@ref fatty acid percentage 43103 C12:0 1.1 43103 C14:0 4.8 43103 C16:1ω9c 1.6 43103 C16:0 20.1 43103 C17:1ω8c 1.1 43103 C17:0 1 43103 C18:1ω9c 45.4 43103 C18:0 4.4 43103 C20:1ω9c 2.4 43103 C16:1ω6c / C16:1ω7c / C15:0 iso 2OH 14.9 - type of FA analysis: whole cell analysis
- incubation medium: Columbia agar with 5 % defibrinated sheep blood
- agar/liquid: agar
- incubation temperature: 25
- incubation time: 2
- system: MIS MIDI
- cutoff value:
Isolation, sampling and environmental information
isolation
@ref | sample type | host species | geographic location | country | origin.country | continent | enrichment culture | enrichment culture duration | enrichment culture temperature |
---|---|---|---|---|---|---|---|---|---|
24393 | oral cavity of a healthy Chinese cobra Naja atra | Naja atra | Hong Kong, Queen Mary Hospital | China | CHN | Asia | |||
43103 | oral cavities of healthy Chinese cobras (Naja atra) | Hong Kong | China | CHN | Asia | Columbia agar (Oxoid) with 5 % defibrinated sheep blood | 2 days | 25 | |
67770 | Oral cavity of two Chinese cobras (Naja atra) | Naja atra | Hong Kong | China | CHN | Asia |
isolation source categories
Cat1 | Cat2 |
---|---|
#Host | #Reptilia |
#Host Body-Site | #Oral cavity and airways |
taxonmaps
- @ref: 69479
- File name: preview.99_52063.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_1974;97_2373;98_2929;99_52063&stattab=map
- Last taxonomy: Tsukamurella serpentis
- 16S sequence: KT581020
- Sequence Identity:
- Total samples: 297
- soil counts: 60
- aquatic counts: 78
- animal counts: 118
- plant counts: 41
Safety information
risk assessment
- @ref: 24393
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24393
- description: Tsukamurella serpentis strain HKU54 16S ribosomal RNA gene, partial sequence
- accession: KT581020
- length: 1407
- database: ena
- NCBI tax ID: 1750603
GC content
@ref | GC-content | method |
---|---|---|
43103 | 69.2 | |
24393 | 69.2 | thermal denaturation, midpoint method (Tm) |
67770 | 66.7-71.7 | thermal denaturation, midpoint method (Tm) |
External links
@ref: 24393
culture collection no.: DSM 100915, JCM 31017
straininfo link
- @ref: 90919
- straininfo: 407697
literature
- topic: Phylogeny
- Pubmed-ID: 27257031
- title: Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra).
- authors: Tang Y, Teng JLL, Cheung CLW, Ngan AHY, Huang Y, Wong SSY, Yip EKT, Ng KHL, Que TL, Lau SKP, Woo PCY
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.001187
- year: 2016
- mesh: Actinomycetales/*classification/genetics/isolation & purification, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Elapidae/*microbiology, Fatty Acids/chemistry, Hong Kong, Mouth/*microbiology, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Transcriptome
Reference
@id | authors | title | doi/url | catalogue | journal | pubmed |
---|---|---|---|---|---|---|
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
24393 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-100915 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 100915) | |||
43103 | Ying Tang, Jade L. L. Teng, Candy L. W. Cheung, Antonio H. Y. Ngan, Yi Huang, Samson S. Y. Wong, Eric K. T. Yip, Kenneth H. L. Ng, Tak-Lun Que, Susanna K. P. Lau, Patrick C. Y. Woo | Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra) | 10.1099/ijsem.0.001187 | IJSEM 66: 3329-3336 2016 | 27257031 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
90919 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID407697.1 |