Strain identifier

BacDive ID: 132565

Type strain: Yes

Species: Actinomadura adrarensis

Strain Designation: ACD12

Strain history: CECT 8842 <-- DSM 46745 <-- N. Sabaou; Ecole Normale Supérieure, Algeria; ACD12.

NCBI tax ID(s): 1819600 (species)

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General

@ref: 24354

BacDive-ID: 132565

DSM-Number: 46745

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative

description: Actinomadura adrarensis ACD12 is a mesophilic, Gram-negative bacterium that was isolated from Saharan soil, sandy loam from a palm grove.

NCBI tax id

  • NCBI tax id: 1819600
  • Matching level: species

strain history

@refhistory
24354<- N. Sabaou, Alger, Algeria; ACD12
67770CECT 8842 <-- DSM 46745 <-- N. Sabaou; Ecole Normale Supérieure, Algeria; ACD12.

doi: 10.13145/bacdive132565.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Streptosporangiales
  • family: Thermomonosporaceae
  • genus: Actinomadura
  • species: Actinomadura adrarensis
  • full scientific name: Actinomadura adrarensis Lahoum et al. 2016

@ref: 24354

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Thermomonosporaceae

genus: Actinomadura

species: Actinomadura adrarensis

full scientific name: Actinomadura adrarensis Lahoum et al. 2016

strain designation: ACD12

type strain: yes

Morphology

cell morphology

  • @ref: 43944
  • gram stain: negative
  • motility: no

pigmentation

  • @ref: 43944
  • production: no
  • name: diffusible pigments

multimedia

  • @ref: 24354
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_46745.jpg
  • caption: Medium 553 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
24354TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium535.pdf
43944Bennett's agaryes
43944Humic acid-vitamin agaryes
43944ISP 2 agaryes
43944ISP 3 agaryes
43944ISP 4 agaryes
24354GPHF-MEDIUM (DSMZ Medium 553)yeshttps://mediadive.dsmz.de/medium/553Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43944positiveoptimum30mesophilic
24354positivegrowth28mesophilic
67770positivegrowth28mesophilic

culture pH

  • @ref: 43944
  • ability: positive
  • type: optimum
  • pH: 8

Physiology and metabolism

halophily

  • @ref: 43944
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 0 %(w/v)

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4394415824D-fructose-carbon source
4394412936D-galactose-carbon source
4394417634D-glucose-carbon source
4394417716lactose-carbon source
4394416899D-mannitol-carbon source
4394428053melibiose-carbon source
4394416634raffinose-carbon source
4394417924D-sorbitol-carbon source
4394465327D-xylose-carbon source
4394430849L-arabinose-carbon source
4394462345L-rhamnose-carbon source
4394416708adenine-degradation
4394418305arbutin-degradation
4394416150benzoate-degradation
4394416235guanine-degradation
4394417895L-tyrosine-degradation
4394430623oxalate-degradation
4394417272propionate-degradation
4394428017starch-degradation
4394430031succinate-degradation
4394430929tartrate-degradation
4394415318xanthine-degradation
4394417632nitrate-reduction
4394415963ribitol+carbon source
4394417057cellobiose+carbon source
4394417306maltose+carbon source
4394416024D-mannose+carbon source
4394416988D-ribose+carbon source
4394416551D-trehalose+carbon source
43944casein+degradation
439444853esculin+degradation
439445291gelatin+degradation
4394417368hypoxanthine+degradation
4394415361pyruvate+degradation
4394453426tween 80+degradation

fatty acid profile

  • fatty acids

    @reffatty acidpercentage
    43944C13:00.2
    43944C14:01.7
    43944C15:011.1
    43944C15:0 3OH0.9
    43944C16:022.2
    4394410-methyl C16:02.5
    43944C17:07.1
    4394410-methyl C17:015.2
    43944C17:1ω9c11.5
    43944C18:01.4
    4394410-methyl C18:09.3
    43944C18:1ω9c5
    43944C19:1ω9c4.2
    43944C16:0 iso4.5
    43944iso-C16:1 F0.5
    43944C16:1 iso H0.6
    43944C18:0 iso0.5
    43944C18:1 iso H0.4
  • type of FA analysis: whole cell analysis
  • incubation medium: ISP 2 medium
  • agar/liquid: liquid
  • incubation temperature: 30
  • incubation time: 7
  • software version: Sherlock 6.1
  • library/peak naming table: TSBA6
  • system: MIS MIDI
  • method/protocol: Sasser, 1990

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitudeenrichment cultureenrichment culture compositionenrichment culture durationenrichment culture temperature
24354Saharan soil, sandy loam from a palm groveAdrar province, Bouda region (27° 52' N, 0° 17' W)AlgeriaDZAAfrica27.8667-0.2833
43944Saharan sandy loam soil sample collected from palm grove of Bouda region, Adrar province, southern AlgeriaBouda region, Adrar provinceAlgeriaDZAAfrica27.8667-0.283333Chitin-vitamin agarsupplemented 80 µg/ mL cycloheximide21 days30
67770Saharan soil from Adrar Provincesouth of AlgeriaAlgeriaDZAAfrica

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Sandy
#Engineered#Agriculture#Grove (Orchard)
#Environmental#Terrestrial#Desert
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 24354
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
24354Actinomadura adrarensis strain ACD12 16S ribosomal RNA gene, partial sequenceKU3569421484ena1819600
24354Actinomadura adrarensis strain ACD12 16S ribosomal RNA, partial sequenceNR_1445701484nuccore1819600

External links

@ref: 24354

culture collection no.: DSM 46745, CECT 8842, JCM 31696

straininfo link

  • @ref: 90882
  • straininfo: 405248

literature

  • topic: Phylogeny
  • Pubmed-ID: 27117880
  • title: Actinomadura adrarensis sp. nov., an actinobacterium isolated from Saharan soil.
  • authors: Lahoum A, Bouras N, Verheecke C, Mathieu F, Schumann P, Sproer C, Klenk HP, Sabaou N
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.001114
  • year: 2016
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Africa, Northern, Algeria, Bacterial Typing Techniques, Base Composition, Cell Wall/chemistry, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24354Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-46745Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 46745)
43944Abdelhadi Lahoum, Noureddine Bouras, Carol Verheecke, Florence Mathieu, Peter Schumann, Cathrin Spröer, Hans-Peter Klenk, Nasserdine SabaouActinomadura adrarensis sp. nov., an actinobacterium isolated from Saharan soil10.1099/ijsem.0.001114IJSEM 66: 2724-2729 201627117880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
90882Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID405248.1