Strain identifier
BacDive ID: 132559
Type strain: 
Species: Nocardioides pakistanensis
Strain history: W.-J. Li; Yunnan Inst. of Microbiol., Yunnan Univ., China; NCCP-1340.
NCBI tax ID(s): 1682732 (species)
version 8.1 (current version)
General
@ref: 24348
BacDive-ID: 132559
DSM-Number: 29942
keywords: 16S sequence, Bacteria, mesophilic
description: Nocardioides pakistanensis DSM 29942 is a mesophilic bacterium that was isolated from water from a hot water spring.
NCBI tax id
- NCBI tax id: 1682732
- Matching level: species
strain history
| @ref | history |
|---|---|
| 24348 | <- W.-J. Li, YIM; NCCP-1340 <- Arshia, YIM |
| 67770 | W.-J. Li; Yunnan Inst. of Microbiol., Yunnan Univ., China; NCCP-1340. |
doi: 10.13145/bacdive132559.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Propionibacteriales
- family: Nocardioidaceae
- genus: Nocardioides
- species: Nocardioides pakistanensis
- full scientific name: Nocardioides pakistanensis Amin et al. 2016
@ref: 24348
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Nocardioidaceae
genus: Nocardioides
species: Nocardioides pakistanensis
full scientific name: Nocardioides pakistanensis Amin et al. 2016
type strain: yes
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 24348 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | yes | https://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium65.pdf | |
| 24348 | R2A MEDIUM (DSMZ Medium 830) | yes | https://mediadive.dsmz.de/medium/830 | Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 24348 | positive | growth | 28 | mesophilic |
| 67770 | positive | growth | 28 | mesophilic |
Physiology and metabolism
observation
- @ref: 67770
- observation: quinones: MK-8(H4)
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | latitude | longitude |
|---|---|---|---|---|---|---|---|
| 24348 | water from a hot water spring | Kotli, Tatta Pani (35° 47' N, 74° 54' E) | Pakistan | PAK | Asia | 35.7833 | 74.9 |
| 67770 | Soil from Tatta Pani hot water spring | Kotli, Azad Jammu & Kashmir | Pakistan | PAK | Asia |
isolation source categories
| Cat1 | Cat2 |
|---|---|
| #Environmental | #Aquatic |
| #Condition | #Thermophilic (>45°C) |
Safety information
risk assessment
- @ref: 24348
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24348
- description: Nocardioides pakistanensis gene for 16S ribosomal RNA, partial sequence
- accession: LC065367
- length: 1498
- database: ena
- NCBI tax ID: 1682732
GC content
- @ref: 24348
- GC-content: 71.8
- method: high performance liquid chromatography (HPLC)
External links
@ref: 24348
culture collection no.: DSM 29942, JCM 30630, NCCP 1340
straininfo link
- @ref: 90876
- straininfo: 402810
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 27170166 | Nocardioides pakistanensis sp. nov., isolated from a hot water spring of Tatta Pani in Pakistan. | Amin A, Ahmed I, Habib N, Abbas S, Xiao M, Hozzein WN, Li WJ | Antonie Van Leeuwenhoek | 10.1007/s10482-016-0711-8 | 2016 | Actinomycetales/*classification/genetics/*isolation & purification/metabolism, Aerobiosis, Bacterial Typing Techniques, Diaminopimelic Acid/analysis, Fatty Acids/metabolism, Hot Springs/*microbiology, Nucleic Acid Hybridization, Pakistan, Phenotype, Phospholipids/metabolism, Phylogeny, Sequence Analysis, DNA, Sodium Chloride/metabolism, Water Microbiology | Metabolism |
| Phylogeny | 30603803 | Nocardioides speluncae sp. nov., a novel actinobacterium isolated from a karstic subterranean environment sample. | Fang BZ, Han MX, Jiao JY, Zhang XT, Xie YG, Hozzein WN, Alkhalifah DHM, Xiao M, Li WJ | Antonie Van Leeuwenhoek | 10.1007/s10482-018-01217-9 | 2019 | Actinomycetales/classification/genetics/*isolation & purification/metabolism, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry/metabolism, Phylogeny, RNA, Ribosomal, 16S/genetics, *Soil Microbiology | Metabolism |
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 24348 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29942 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29942) | |
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
| 90876 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402810.1 |