Strain identifier

BacDive ID: 132528

Type strain: Yes

Species: Brucella vulpis

Strain Designation: F60

Strain history: <- H. C. Scholz, Bundeswehr Institute of Microbiology, Munich, Germany <- E. Hofer <- R. Posch <- K. Reisp, Austrian Agency for Health and Food Safety; F60

NCBI tax ID(s): 981386 (species)

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General

@ref: 24317

BacDive-ID: 132528

DSM-Number: 101715

keywords: genome sequence, Bacteria, aerobe, mesophilic, Gram-negative, coccus-shaped, colony-forming

description: Brucella vulpis F60 is an aerobe, mesophilic, Gram-negative bacterium that forms circular colonies and was isolated from mandibular lymph nodes of a red foxes .

NCBI tax id

  • NCBI tax id: 981386
  • Matching level: species

strain history

  • @ref: 24317
  • history: <- H. C. Scholz, Bundeswehr Institute of Microbiology, Munich, Germany <- E. Hofer <- R. Posch <- K. Reisp, Austrian Agency for Health and Food Safety; F60

doi: 10.13145/bacdive132528.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Brucellaceae
  • genus: Brucella
  • species: Brucella vulpis
  • full scientific name: Brucella vulpis Scholz et al. 2016

@ref: 24317

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Brucellaceae

genus: Brucella

species: Brucella vulpis

full scientific name: Brucella vulpis Scholz et al. 2016

strain designation: F60

type strain: yes

Morphology

cell morphology

  • @ref: 43648
  • gram stain: negative
  • cell length: 0.4-1.3 µm
  • cell width: 0.5-0.7 µm
  • cell shape: coccus-shaped
  • motility: no

colony morphology

  • @ref: 43648
  • type of hemolysis: gamma
  • colony size: 0.5 mm
  • colony color: Greyish
  • colony shape: circular
  • incubation period: 3 days
  • medium used: Columbia Agar

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43648Columbia Agaryes
24317COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base

culture temp

@refgrowthtypetemperaturerange
43648positivegrowth30-37mesophilic
24317positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 43648
  • oxygen tolerance: aerobe

spore formation

  • @ref: 43648
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4364817108D-arabinose-assimilation
4364818287L-fucose-assimilation
4364812936D-galactose-assimilation
4364865327D-xylose-assimilation
4364830849L-arabinose-assimilation
43648911224-nitrophenyl alpha-D-glucopyranoside-assimilation
43648901484-nitrophenyl beta-D-xylopyranoside-assimilation
43648174404-nitrophenyl phosphate-assimilation
4364817128adipate-assimilation
4364815963ribitol-assimilation
4364822653asparagine-assimilation
436488295beta-hydroxybutyrate-assimilation
436483122bis-4-nitrophenyl phosphate-assimilation
4364815570D-alanine-assimilation
4364817634D-glucose-assimilation
4364827947D-histidine-assimilation
4364816313D-proline-assimilation
4364816988D-ribose-assimilation
4364829806fumarate-assimilation
4364817859glutaric acid-assimilation
4364815428glycine-assimilation
4364829805glycolate-assimilation
4364816977L-alanine-assimilation
4364829985L-glutamate-assimilation
4364817203L-proline-assimilation
4364817115L-serine-assimilation
4364824996lactate-assimilation
4364817306maltose-assimilation
4364836986mesaconate-assimilation
4364818394palatinose-assimilation
4364817272propionate-assimilation
4364817992sucrose-assimilation
4364817632nitrate-reduction
4364816301nitrite-reduction
4364848095(-)-D-fructose+assimilation
43648903434-nitrophenyl N-acetyl-beta-D-glucosaminide+assimilation
4364817596inosine+assimilation
4364821796-deoxy-d-galactose+/-assimilation
4364830089acetate+/-assimilation
4364816708adenine+/-assimilation
4364816523D-serine+/-assimilation
4364817113erythritol+/-assimilation
4364836655glyoxylate+/-assimilation
4364816467L-arginine+/-assimilation
4364817196L-asparagine+/-assimilation
4364816283L-cystine+/-assimilation
4364817268myo-inositol+/-assimilation
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836916899D-mannitol-assimilation
6836930849L-arabinose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
6838129016arginine+hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose-builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose-builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
4364816136hydrogen sulfideno
4364815688acetoinyes
6836935581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
4364815688acetoin+
6838115688acetoin-
6836935581indole-

enzymes

@refvalueactivityec
43648urease+3.5.1.5
43648catalase+1.11.1.6
43648cytochrome oxidase-1.9.3.1
43648nitrate reductase-1.7.99.4
68381urease+3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase+
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase+3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase+3.5.3.6
68369cytochrome oxidase-1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
24317----+---+/--+/-----------
24317----+---+/--+-+/---------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
24317+----+-----------+---+---------+
24317+----+-----------+---+---------+

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinent
24317mandibular lymph nodes of a red foxes (Vulpes vulpes)Vulpes vulpesLower AustriaAustriaAUTEurope
43648mandibular lymph nodes of a red fox (Vulpes vulpes)Lower AustriaAustriaAUTEurope

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Oral cavity and airways
#Host#Mammals#Canidae (Dog)
#Host Body-Site#Organ#Lymph node

Sequence information

Genome sequences

  • @ref: 66792
  • description: Brucella vulpis F60
  • accession: GCA_900000005
  • assembly level: complete
  • database: ncbi
  • NCBI tax ID: 981386

GC content

@refGC-contentmethod
4364857.2
2431757.2sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno67.01no
flagellatedno87.715no
gram-positiveno98.537yes
anaerobicno98.408no
aerobicyes90.544yes
halophileno87.136no
spore-formingno96.402yes
glucose-fermentno89.283yes
thermophileno93.354yes
glucose-utilyes83.801yes

External links

@ref: 24317

culture collection no.: DSM 101715, BCCN 09-2

straininfo link

  • @ref: 90854
  • straininfo: 403993

literature

  • topic: Phylogeny
  • Pubmed-ID: 26928956
  • title: Brucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes).
  • authors: Scholz HC, Revilla-Fernandez S, Dahouk SA, Hammerl JA, Zygmunt MS, Cloeckaert A, Koylass M, Whatmore AM, Blom J, Vergnaud G, Witte A, Aistleitner K, Hofer E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijsem.0.000998
  • year: 2016
  • mesh: Animals, Austria, Bacterial Typing Techniques, Bacteriophage Typing, Base Composition, Brucella/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Foxes/*microbiology, Lymph Nodes/*microbiology, *Phylogeny, Sequence Analysis, DNA
  • topic2: Transcriptome

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24317Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-101715Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 101715)
43648Holger C. Scholz, Sandra Revilla-Fernandez, Sascha Al Dahouk, Jens A. Hammerl, Michel S. Zygmunt, Axel Cloeckaert, Mark Koylass, Adrian M. Whatmore, Jochen Blom, Gilles Vergnaud, Angela Witte, Karin Aistleitner, Erwin HoferBrucella vulpis sp. nov., isolated from mandibular lymph nodes of red foxes (Vulpes vulpes)10.1099/ijsem.0.000998IJSEM 66: 2090-2098 201626928956
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
68381Automatically annotated from API rID32STR
90854Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID403993.1