Strain identifier
BacDive ID: 132478
Type strain: 
Species: Vallitalea guaymasensis
Strain Designation: L81
Strain history: I. H. Steen; Centre for Geobiol., Univ. of Bergen, Norway; L81.
NCBI tax ID(s): 1675854 (species)
version 8.1 (current version)
General
@ref: 24267
BacDive-ID: 132478
DSM-Number: 29592
keywords: genome sequence, 16S sequence, Bacteria, obligate anaerobe, chemoorganoheterotroph, Gram-positive, motile, rod-shaped
description: Vallitalea guaymasensis L81 is an obligate anaerobe, chemoorganoheterotroph, Gram-positive bacterium that was isolated from chimney of a deep-sea black smoker, approximately 2400 m depth.
NCBI tax id
- NCBI tax id: 1675854
- Matching level: species
strain history
| @ref | history |
|---|---|
| 24267 | <- A. Schouw, Univ. Bergen, Norway; L81 <- A. Schouw {2012} |
| 67770 | I. H. Steen; Centre for Geobiol., Univ. of Bergen, Norway; L81. |
doi: 10.13145/bacdive132478.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Vallitaleaceae
- genus: Vallitalea
- species: Vallitalea guaymasensis
- full scientific name: Vallitalea guaymasensis Lakhal et al. 2013
synonyms
- @ref: 20215
- synonym: Abyssivirga alkaniphila
@ref: 24267
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Lachnospiraceae
genus: Abyssivirga
species: Abyssivirga alkaniphila
full scientific name: Abyssivirga alkaniphila Schouw et al. 2016
strain designation: L81
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 43989 | positive | 2.0-5.0 µm | 0.5 µm | rod-shaped | yes | |
| 69480 | positive | 100 | ||||
| 69480 | yes | 94.828 |
Culture and growth conditions
culture medium
- @ref: 24267
- name: MARINE DESULFOVIBRIO COMPLEX MEDIUM (DSMZ Medium 1040)
- growth: yes
- link: https://mediadive.dsmz.de/medium/1040
- composition: Name: MARIDESULFOVIBRIO MEDIUM (DSMZ Medium 1040; with strain-specific modifications) Composition: Sea Salt 34.9301 g/l D-Glucose 2.99401 g/l Yeast extract 0.998004 g/l Na2CO3 0.998004 g/l Na2S x 9 H2O 0.299401 g/l MgSO4 x 7 H2O 0.0299401 g/l NaCl 0.00998004 g/l MnSO4 x H2O 0.00499002 g/l (NH4)2Ni(SO4)2 x 6 H2O 0.00279441 g/l ZnSO4 x 7 H2O 0.00179641 g/l CoCl2 x 6 H2O 0.00179641 g/l FeSO4 x 7 H2O 0.000998004 g/l CaCl2 x 2 H2O 0.000998004 g/l Sodium resazurin 0.000499002 g/l AlK(SO4)2 x 12 H2O 0.000179641 g/l CuSO4 x 5 H2O 9.98004e-05 g/l H3BO3 9.98004e-05 g/l Na2MoO4 x 2 H2O 9.98004e-05 g/l Na2WO4 x 2 H2O 9.98004e-05 g/l Na2SeO4 9.98004e-05 g/l Pyridoxine hydrochloride 9.98004e-05 g/l p-Aminobenzoic acid 4.99002e-05 g/l Calcium D-(+)-pantothenate 4.99002e-05 g/l Nicotinic acid 4.99002e-05 g/l Riboflavin 4.99002e-05 g/l Thiamine HCl 4.99002e-05 g/l (DL)-alpha-Lipoic acid 4.99002e-05 g/l Folic acid 1.99601e-05 g/l Biotin 1.99601e-05 g/l Vitamin B12 9.98004e-07 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 43989 | positive | growth | 15-42 | |
| 43989 | positive | optimum | 37 | mesophilic |
| 24267 | positive | growth | 37 | mesophilic |
| 67770 | positive | growth | 37 | mesophilic |
culture pH
- @ref: 43989
- ability: positive
- type: optimum
- pH: 7.0-8.2
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 43989 | obligate anaerobe | |
| 69480 | anaerobe | 97.922 |
nutrition type
- @ref: 43989
- type: chemoorganoheterotroph
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 43989 | no | |
| 69481 | no | 97 |
| 69480 | yes | 100 |
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 43989 | NaCl | positive | growth | 0.5-6 %(w/v) |
| 43989 | NaCl | positive | optimum | 3 %(w/v) |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 43989 | 30089 | acetate | - | growth |
| 43989 | 16094 | thiosulfate | + | electron acceptor |
| 43989 | 18024 | D-galacturonic acid | + | growth |
| 43989 | 28757 | fructose | + | growth |
| 43989 | 28260 | galactose | + | growth |
| 43989 | 17234 | glucose | + | growth |
| 43989 | 17754 | glycerol | + | growth |
| 43989 | 24996 | lactate | + | growth |
| 43989 | 17306 | maltose | + | growth |
| 43989 | 17309 | pectin | + | growth |
| 43989 | peptone | + | growth | |
| 43989 | 62969 | polygalacturonic acid | + | growth |
| 43989 | 33942 | ribose | + | growth |
| 43989 | yeast extract | + | growth |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | latitude | longitude | country | enrichment culture | enrichment culture composition |
|---|---|---|---|---|---|---|---|
| 24267 | chimney of a deep-sea black smoker, approximately 2400 m depth | Arctic Mid-Ocean Ridge, Loki’s Castle vent field | 73.55 | 8.15 | |||
| 43989 | methane-producing, hydro- carbon-degrading microbial consortium enriched from a black smoker at the Loki's Castle vent field, Arctic Mid-Ocean Ridge | Loki's Castle vent field | 73.55 | 8.15 | Arctic Ocean | Met II growth medium | 22 g/L NaCl, 0.14 g/L CaCl2.2H2O, 0.34 g/L KCl, 0.5 g NH4Cl, 5.5 g/L MgCl2.6H2O, 0.14 g/L K2HPO4.3H2O, 0.2 g/L yeast extract, 10 ml trace element solution (DSMZ medium 141) and 0.5 ml resazurin solution (0.02 %), 10 ml iron solution [0.2 g (NH4)2Fe(SO4)2.6H2O dissolved in 0.2 ml 1 M HCl and added to 100 ml distilled water], 0.5 g cysteine hydrochloride, 10 ml vitamin solution, 30 ml 1.0 M NaHCO3, 5 ml 0.5 M Na2S, pH 7.2 |
| 67770 | Biofilm sampled from a black smoker chimney at the Loki |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Hydrothermal vent |
| #Environmental | #Biofilm |
taxonmaps
- @ref: 69479
- File name: preview.99_27294.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_922;96_13533;97_16476;98_20440;99_27294&stattab=map
- Last taxonomy: Eubacteriales
- 16S sequence: KP233895
- Sequence Identity:
- Total samples: 216
- soil counts: 16
- aquatic counts: 115
- animal counts: 85
Safety information
risk assessment
- @ref: 24267
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 24267
- description: Abyssivirga alkaniphila strain L81 16S ribosomal RNA gene, partial sequence
- accession: KP233895
- length: 1520
- database: ena
- NCBI tax ID: 1675854
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Vallitalea guaymasensis strain L81 | 1185412.3 | wgs | patric | 1185412 |
| 66792 | Vallitalea guaymasensis L81 | GCA_003313305 | contig | ncbi | 1185412 |
GC content
- @ref: 24267
- GC-content: 31.7
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 97 | no |
| motile | yes | 85.806 | yes |
| gram-positive | yes | 76.406 | yes |
| anaerobic | yes | 98.315 | yes |
| aerobic | no | 97.081 | no |
| halophile | no | 83.518 | no |
| spore-forming | yes | 64.07 | yes |
| flagellated | yes | 50.838 | no |
| thermophile | no | 94.552 | no |
| glucose-util | yes | 90.98 | yes |
| glucose-ferment | yes | 55.908 | no |
External links
@ref: 24267
culture collection no.: DSM 29592, JCM 30920
literature
- topic: Phylogeny
- Pubmed-ID: 26822139
- title: Abyssivirga alkaniphila gen. nov., sp. nov., an alkane-degrading, anaerobic bacterium from a deep-sea hydrothermal vent system, and emended descriptions of Natranaerovirga pectinivora and Natranaerovirga hydrolytica.
- authors: Schouw A, Leiknes Eide T, Stokke R, Pedersen RB, Steen IH, Bodtker G
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijsem.0.000934
- year: 2016
- mesh: Alkanes/*metabolism, Arctic Regions, Bacterial Typing Techniques, Base Composition, Biodegradation, Environmental, Clostridiales/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Glycolipids/chemistry, Hydrothermal Vents/*microbiology, Molecular Sequence Data, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA
- topic2: Genetics
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 24267 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-29592 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 29592) | |||
| 43989 | Anders Schouw, Tove Leiknes Eide, Runar Stokke, Rolf Birger Pedersen, Ida Helene Steen, Gunhild Bødtker | Abyssivirga alkaniphila gen. nov., sp. nov., an alkane-degrading, anaerobic bacterium from a deep-sea hydrothermal vent system, and emended descriptions of Natranaerovirga pectinivora and Natranaerovirga hydrolytica | 10.1099/ijsem.0.000934 | IJSEM 66: 1724-1734 2016 | 26822139 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | Predictions based on the model GenomeNet Sporulation v. 1 | 10.21203/rs.3.rs-2527258/v1 |