Strain identifier

BacDive ID: 132460

Type strain: Yes

Species: Megasphaera butyrica

Strain Designation: Sanger 24, Sanger_24

Strain history: <- H. Browne, Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom; Sanger_24

NCBI tax ID(s): 2023260 (species)

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General

@ref: 24249

BacDive-ID: 132460

DSM-Number: 102144

keywords: genome sequence, Bacteria, mesophilic

description: Megasphaera butyrica Sanger 24 is a mesophilic bacterium that was isolated from faeces from healthy human.

NCBI tax id

  • NCBI tax id: 2023260
  • Matching level: species

strain history

  • @ref: 24249
  • history: <- H. Browne, Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom; Sanger_24

doi: 10.13145/bacdive132460.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Negativicutes
  • order: Veillonellales
  • family: Veillonellaceae
  • genus: Megasphaera
  • species: Megasphaera butyrica
  • full scientific name: Megasphaera butyrica Hitch et al. 2022

@ref: 24249

domain: Bacteria

phylum: Firmicutes

class: Negativicutes

order: Veillonellales

family: Veillonellaceae

genus: Megasphaera

species: Megasphaera butyrica

full scientific name: Megasphaera butyrica Hitch et al. 2022

strain designation: Sanger 24, Sanger_24

type strain: yes

Culture and growth conditions

culture medium

  • @ref: 24249
  • name: CHOPPED MEAT MEDIUM (DSMZ Medium 78)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/78
  • composition: Name: CHOPPED MEAT MEDIUM (DSMZ Medium 78) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l Ethanol 9.5 g/l (optional) K2HPO4 5.0 g/l Yeast extract 5.0 g/l L-Cysteine HCl 0.5 g/l Haemin 0.005 g/l (optional) Resazurin 0.001 g/l Vitamin K3 0.0005 g/l (optional) Vitamin K1 (optional) NaOH (optional) Distilled water

culture temp

  • @ref: 24249
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase-
68380glutamyl-glutamate arylamidase-
68380histidine arylamidase-
68380glycin arylamidase-
68380alanine arylamidase-3.4.11.2
68380tyrosine arylamidase-
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase-3.4.11.1
68380phenylalanine arylamidase-
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase-3.4.11.5
68380L-arginine arylamidase-
68380alkaline phosphatase+3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
24249----------------+------------

Isolation, sampling and environmental information

isolation

  • @ref: 24249
  • sample type: faeces from healthy human
  • country: United Kingdom
  • origin.country: GBR
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body Product#Gastrointestinal tract#Feces (Stool)

Sequence information

Genome sequences

  • @ref: 66792
  • description: Megasphaera butyrica Sanger_24
  • accession: GCA_025567065
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 2981791

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno91.675no
flagellatedno96.675no
gram-positiveno97.33no
anaerobicyes99.693no
aerobicno98.446no
halophileno80.282no
spore-formingno93.888no
glucose-fermentyes59.762no
thermophileno97.145no
glucose-utilyes71.588no

External links

@ref: 24249

culture collection no.: DSM 102144, JCM 34811

straininfo link

  • @ref: 90797
  • straininfo: 406767

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24249Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-102144Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 102144)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68380Automatically annotated from API rID32A
90797Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID406767.1