Strain identifier

BacDive ID: 132451

Type strain: Yes

Species: Paenibacillus bovis

Strain Designation: BD 3526, BD3526

Strain history: <- Z. Wu, State Key Lab. of Dairy Biotechnol, Shanghai, China; BD3526

NCBI tax ID(s): 1616788 (species)

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General

@ref: 24240

BacDive-ID: 132451

DSM-Number: 28815

keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, Gram-positive, motile, rod-shaped, colony-forming

description: Paenibacillus bovis BD 3526 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from raw yak Bos grunniens milk.

NCBI tax id

  • NCBI tax id: 1616788
  • Matching level: species

strain history

  • @ref: 24240
  • history: <- Z. Wu, State Key Lab. of Dairy Biotechnol, Shanghai, China; BD3526

doi: 10.13145/bacdive132451.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Paenibacillaceae
  • genus: Paenibacillus
  • species: Paenibacillus bovis
  • full scientific name: Paenibacillus bovis Gao et al. 2016

@ref: 24240

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Paenibacillaceae

genus: Paenibacillus

species: Paenibacillus bovis

full scientific name: Paenibacillus bovis Gao et al. 2016

strain designation: BD 3526, BD3526

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityflagellum arrangementconfidence
43738positive1.8-6.9 µm0.6-1.2 µmrod-shapedyesperitrichous
69480yes95.485
69480positive100

colony morphology

  • @ref: 43738
  • colony color: Opaque, orange to red
  • colony shape: circular
  • incubation period: 2 days
  • medium used: TYC agar plates

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
43738TYV agaryes
24240R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
43738positivegrowth10-40
43738positiveoptimum30-35mesophilic
24240positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
43738positivegrowth5-8.5alkaliphile
43738positiveoptimum6-6.5

Physiology and metabolism

oxygen tolerance

  • @ref: 43738
  • oxygen tolerance: aerobe

spore formation

@reftype of sporespore formationconfidence
43738endosporeyes
69481yes100
69480yes99.419

halophily

@refsaltgrowthtested relationconcentration
43738NaClpositivegrowth0-10 %(w/v)
43738NaClpositiveoptimum0-2 %(w/v)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4373816947citrate-assimilation
43738581435-dehydro-D-gluconate-builds acid from
4373815963ribitol-builds acid from
4373817108D-arabinose-builds acid from
4373818333D-arabitol-builds acid from
4373828847D-fucose-builds acid from
4373862318D-lyxose-builds acid from
4373817924D-sorbitol-builds acid from
4373816443D-tagatose-builds acid from
4373816813galactitol-builds acid from
4373817113erythritol-builds acid from
4373817754glycerol-builds acid from
4373817268myo-inositol-builds acid from
4373818403L-arabitol-builds acid from
4373818287L-fucose-builds acid from
4373862345L-rhamnose-builds acid from
4373817266L-sorbose-builds acid from
4373865328L-xylose-builds acid from
437386731melezitose-builds acid from
43738320061methyl alpha-D-glucopyranoside-builds acid from
4373843943methyl alpha-D-mannoside-builds acid from
4373817151xylitol-builds acid from
4373817108D-arabinose-fermentation
4373817754glycerol-fermentation
4373817268myo-inositol-fermentation
4373837657methyl D-glucoside-fermentation
4373826546rhamnose-fermentation
4373830911sorbitol-fermentation
4373829016arginine-hydrolysis
4373825094lysine-hydrolysis
4373818257ornithine-hydrolysis
4373816199urea-hydrolysis
4373818024D-galacturonic acid-oxidation
4373862345L-rhamnose-oxidation
4373835418n-acetylneuraminate-oxidation
43738168082-dehydro-D-gluconate+builds acid from
4373827613amygdalin+builds acid from
4373818305arbutin+builds acid from
4373817057cellobiose+builds acid from
4373815824D-fructose+builds acid from
4373812936D-galactose+builds acid from
4373817634D-glucose+builds acid from
4373816899D-mannitol+builds acid from
4373816024D-mannose+builds acid from
4373816988D-ribose+builds acid from
4373865327D-xylose+builds acid from
4373828066gentiobiose+builds acid from
4373824265gluconate+builds acid from
4373828087glycogen+builds acid from
4373830849L-arabinose+builds acid from
4373817716lactose+builds acid from
43738maltose hydrate+builds acid from
4373828053melibiose+builds acid from
4373874863methyl beta-D-xylopyranoside+builds acid from
43738506227N-acetylglucosamine+builds acid from
4373816634raffinose+builds acid from
4373817814salicin+builds acid from
4373828017starch+builds acid from
4373817992sucrose+builds acid from
43738168082-dehydro-D-gluconate+fermentation
4373818305arbutin+fermentation
4373816988D-ribose+fermentation
4373824265gluconate+fermentation
43738506227N-acetylglucosamine+fermentation
43738casein+hydrolysis
437384853esculin+hydrolysis
437385291gelatin+hydrolysis
4373828017starch+hydrolysis
4373830089acetate+oxidation
4373813705acetoacetate+oxidation
4373878697D-fructose 6-phosphate+oxidation
4373815895D-galactonic acid lactone+oxidation
4373815740formate+oxidation
4373816977L-alanine+oxidation
4373863154N-acetyl-beta-D-mannosamine+oxidation
4373817309pectin+oxidation
4373817272propionate+oxidation
4373817632nitrate+reduction
4373815443inulin+/-builds acid from
4373827082trehalose+/-builds acid from
4373832528turanose+/-builds acid from
4373815443inulin+/-fermentation
4373874863methyl beta-D-xylopyranoside+/-fermentation
43738synanthrin+/-fermentation
4373827082trehalose+/-fermentation
4373832528turanose+/-fermentation
4373817268myo-inositol+/-oxidation

metabolite production

@refChebi-IDmetaboliteproduction
4373816136hydrogen sulfideno
4373835581indoleno
4373815688acetoinyes

metabolite tests

  • @ref: 43738
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
43738catalase+1.11.1.6
43738cytochrome oxidase-1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcountryorigin.countrycontinentisolation date
24240raw yak Bos grunniens milkBos grunniensTibet, Damxung CountyChinaCHNAsia
43738Raw yak milkTibetChinaCHNAsia2011

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body Product#Fluids#Milk

taxonmaps

  • @ref: 69479
  • File name: preview.99_5642.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15358;96_2714;97_3335;98_4204;99_5642&stattab=map
  • Last taxonomy: Paenibacillus
  • 16S sequence: KM978955
  • Sequence Identity:
  • Total samples: 932
  • soil counts: 60
  • aquatic counts: 147
  • animal counts: 655
  • plant counts: 70

Safety information

risk assessment

  • @ref: 24240
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 24240
  • description: Paenibacillus bovis strain BD3526 16S ribosomal RNA gene, partial sequence
  • accession: KM978955
  • length: 1483
  • database: ena
  • NCBI tax ID: 1616788

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Paenibacillus bovis BD3526GCA_001421015completencbi1616788
66792Paenibacillus sp. BD35261616788.3completepatric1616788
66792Paenibacillus bovis BD35262775507276completeimg1616788

GC content

  • @ref: 24240
  • GC-content: 47.5
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes100no
flagellatedyes74.127yes
flagellatedyes74.127yes
gram-positiveyes87.855yes
gram-positiveyes87.855yes
anaerobicno97.887no
anaerobicno97.887no
aerobicyes81.795yes
aerobicyes81.795yes
halophileno86.579no
halophileno86.579no
spore-formingyes90.784no
spore-formingyes90.784no
thermophileno99.267yes
thermophileno99.267yes
glucose-utilyes90.456no
glucose-utilyes90.456no
motileyes88.806yes
motileyes88.806yes
glucose-fermentno74.415no
glucose-fermentno74.415no

External links

@ref: 24240

culture collection no.: DSM 28815, CGMCC 8333

straininfo link

  • @ref: 90789
  • straininfo: 397293

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny26769366Paenibacillus bovis sp. nov., isolated from raw yak (Bos grunniens) milk.Gao C, Han J, Liu Z, Xu X, Hang F, Wu ZInt J Syst Evol Microbiol10.1099/ijsem.0.0009002016
Pathogenicity27083807Characterization of the levan produced by Paenibacillus bovis sp. nov BD3526 and its immunological activity.Xu X, Gao C, Liu Z, Wu J, Han J, Yan M, Wu ZCarbohydr Polym10.1016/j.carbpol.2016.02.0492016Animals, Cell Proliferation/drug effects, Fructans/biosynthesis/*chemistry/isolation & purification/*pharmacology, Hexosyltransferases/metabolism, Humans, Immunomodulation/*drug effects, Lymphocytes/cytology/drug effects, Mice, Molecular Weight, Monosaccharides/analysis, Paenibacillus/*metabolism, Spleen/immunologyPhylogeny
Phylogeny30687277Fermentation Products of Paenibacillus bovis sp. nov. BD3526 Alleviates the Symptoms of Type 2 Diabetes Mellitus in GK Rats.Qiao Z, Han J, Feng H, Zheng H, Wu J, Gao C, Yang M, You C, Liu Z, Wu ZFront Microbiol10.3389/fmicb.2018.032922019
Phylogeny33044619Isolation and Characterization of a New Fusaricidin-Type Antibiotic Produced by Paenibacillus bovis sp. nov BD3526.Hua B, Feng H, Han J, Qiao Z, Wang X, Zhang Q, Liu Z, Wu ZCurr Microbiol10.1007/s00284-020-02206-w2020Animals, *Anti-Bacterial Agents/pharmacology, Bacillus subtilis, Cattle, *Paenibacillus, Tandem Mass SpectrometryProteome
Pathogenicity33424779Single-Cell Transcriptomics Reveals That Metabolites Produced by Paenibacillus bovis sp. nov. BD3526 Ameliorate Type 2 Diabetes in GK Rats by Downregulating the Inflammatory Response.Qiao Z, Wang X, Zhang H, Han J, Feng H, Wu ZFront Microbiol10.3389/fmicb.2020.5688052020Phylogeny
Metabolism35123741Structure elucidation and immunological activity of a novel exopolysaccharide from Paenibacillus bovis sp. nov BD3526.Zhu S, Han J, Yan Z, Wu Y, Zhang W, Xia W, Feng HCarbohydr Polym10.1016/j.carbpol.2022.1191032022Animals, Cytokines/metabolism, Methylation, Mice, Molecular Structure, NF-kappa B/metabolism, Nitric Oxide/metabolism, *Paenibacillus, Phagocytosis/drug effects, Polysaccharides, Bacterial/*chemistry/*pharmacology, RAW 264.7 Cells, Uronic Acids/chemistryPhylogeny

Reference

@idauthorstitledoi/urlcataloguejournalpubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
24240Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28815Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28815)
43738Caixia Gao, Jin Han, Zhenmin Liu, Xiaofen Xu, Feng Hang, Zhengjun WuPaenibacillus bovis sp. nov., isolated from raw yak (Bos grunniens) milk10.1099/ijsem.0.000900IJSEM 66: 1413-1418 201626769366
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
90789Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397293.1